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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pNY-1A (#RDB07250)

Complete genome of JC virus, PML-type

Clone info. Complete genome of JC virus, PML-type. JCV genomic DNA derived from the brain of an American PML patient.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genomic DNA
Reference of insert sequence D12574 (DDBJ accession number)
Depositor|Developer Yogo, Yoshiaki |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Virus Genes., 8, 99-105 (1994)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Virus Genes., 8, 99-105 (1994))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07250 pNY-1A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pNY-1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07250).

Reference section:

Yogo, Y., Guo, J., Iida, T., Satoh, K., Taguchi, F., Takahashi, H., Hall, W.W., Nagashima, K., Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy. Virus Genes., 8, 99-105 (1994). PMID 7915447. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07250_A3Kj.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB07250_A3Kja.seq check
>07250_07250_A3Kj_2_M13(-40)_B01_04.ab1
    1 CGGAGCGGCG TTATTTCGGA GCTCGGTACC GGGGATCCCA ACACTCTACC CCACCTAAGA
   61 AGAAAAAAAA GGTAGAAGAC CCTAAAGACT TTCCTGTAGA TCTGCATGCA TTCCTCAGTC
  121 AAGCTGTGTT TAGTAATAGA ACTGTTGCTT CTTTTGCTGT GTATACCACT AAAGAAAAAG
  181 CTCAAATTTT ATATAAGAAA CTTATGGAAA AATATTCTGT AACTTTTATA AGTAGACATG
  241 GTTTTGGGGG TCATAATATT TTGTTTTTCT TAACACCACA TAGACATAGA GTGTCAGCAA
  301 TTAATAACTA CTGTCAAAAA CTATGTACCT TTAGTTTTTT AATTTGTAAA GGTGTGAATA
  361 AGGAATACTT GTTTTATAGT GCCCTGTGTA GACAGCCATA TGCAGTAGTG GAAGAAAGTA
  421 TTCAGGGGGG CCTTAAGGAG CATGACTTTA ACCCAGAAGA ACCAGAAGAA ACTAAGCAGG
  481 TTTCATGGAA ATTAGTTACA CAGTATGCCT TGGAAACCAA GTGTGAGGAT GTTTTTTTGC
  541 TTATGGGCAT GTACTTAGAC TTTCAGGAAA ACCCACAGCA ATGCAAAAAA TGTGAAAAAA
  601 AGGATCAGCC AAATCACTTT AACCATCATG AAAAACACTA TTATAATGCC CAAATTTTTG
  661 CAGATAGCAA AAATCAAAAA AGCATTTGCC AGCAGGCTGT TGATACTGTA GCAGCCAAAC
  721 AAAGGGTTGA CAGCATCCAC ATGACCAGAG AAGAAATGTT AGTTGAAAGG TTTAATTTCT
  781 TGCTTGATAA AATGGACTTG ATTTTTGGGG CACATGGCAA TGCTGTTTTA GAGCAATATA
  841 TGGCTGGGGT GGCCTGGATT CATTGCTTGC TGCCTCAAAT GGACACTGTT ATTTATGACT
  901 TTCTGAAATG CATTGTATTA AACATTCCAA AAAAAAAGGT ACTGGCTATT CAAGGGGCCA
  961 ATAGACAGTG GCAAAACTAC TTTAGCTGCA GCTTTACTTG ATCTCTGTGG GGGGAAGTCA
 1021 TTAAATGTTA ATATGCCATT AGAAAGATTA AACTTTGAGT TAGAGTGGTA TAGATCAGTT
 1081 TATGGTTGGT ATTTTGAGGA TGTAAAAGCA CTGGTGCAAG TTCAGGATTT CATCGTCAGC
1141 ATGACCATAA GCCAAATCGG CT
//
Primer:
Sequence file: RDB07250_A3Kjb.seq check
>07250_07250_A3Kj_2_Reverse2_B04_04.ab1
    1 GAACCCGGAA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCTGTGTTT
   61 TCATCATCAC TGGCAAACAT TTCTTCATGG CAAAACAGGT CTTCATCCCA CTTCTCATTA
  121 AATGTATTCC ACCAGGATTC CCATTCATCT GTTCCATAGG TTGGCACCTA AAAAAAAACA
  181 ATTAAGTTTA TTGTAAAAAA CAAAATGCCC TGCAAAAGAA AAATAGTGGT TTACCTTAAA
  241 GCTTTAGATC CCTGTAGGGG GTGTCTCCAA GAACTTTCTC CCAGCAATGA AGAGCTTCTT
  301 GGGTTAAGTC ACACCCAAAC CATTGTCTGA AGCAATCAAA GCAATAGCAA TCTATCCACA
  361 CAAGTGGGCT GCTTCTTAAA AATTTTCTGT TTCTATGCCT TAATTTTAGC ATGCACATTA
  421 AACAGGGGCA ATGCACTGAA GGATTAGTGG CACAGTTAGG CCATTCCTTG CAATAAAGGG
  481 TATCAGAATT AGGAGGAAAA TCACAACCAA CCTCTGAACT ATTCCATGTA CCAAAATCAG
  541 GCTGATGAGC AACTTTTACA CCTTGTTCCA TTTTTTTATA TAAAAAATTC ATTCTCTTCA
  601 TCTTGTCTTC GTCCCCACCT TTATCAGGGT GGAGTTCTTT GCATTTTTTC AGATAAGCTT
  661 TTCTCATGAC AGGAATGTTC CCCCATGCAG ACCTATCAAG GCCTAATAAA TCCATAAGCT
  721 CCATGGATTC CTCCCTATTC AGCACTTTGT CCATTTTAGC TTTTTGCAGC AAAAAATTAC
  781 TGCAAAAAAG GGAAAAACAA GGGAATTTCC CTGGCCTCCT AAAAAGCCTC CACGCCCTTA
  841 CTACTTCTGA GTAAGCTTGG AGGCGGAGGC GGCCTCGGCC TCCTGTATAT ATAAAAAAAA
  901 AGGGAAGGTA GGGAGGAGCT GGCTAAAACT GGATGGGCTG CCCAGCCAAG CATGAGCTCA
  961 TACCTAGGGG AGCCCAACCA GCTGACAGCC AGAGGGAGCC CTGGGCTGCA TGCCACTGGC
 1021 AGTTTATAGT GAAACCCCTT CCCATAGTCC CTTAATCACA AGTAACAAGC ACAGGGAAAG
 1081 TGTCATGTGG CATGCCTGGT CCACCTTGGT TGGGACCATG TTTTTGCGAG CCCAGAAAGC
1141 CTGATATG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yogo, Y., Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy. Virus Genes., 8, 99-105 (1994). PMID 7915447. [link to RRC of NBRP]