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pTky-2a (#RDB07253)

Complete genome of JC virus, PML-type

Clone info. Complete genome of JC virus, PML-type. JCV genomic DNA derived from the brain of a Japanese PML patient.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma JCV genomic DNA
Reference of insert sequence AB038255 (DDBJ accession number)
Depositor|Developer Yogo, Yoshiaki |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Jpn. J. Med. Sci. Biol., 47, 167-178 (1994)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Jpn. J. Med. Sci. Biol., 47, 167-178 (1994))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07253 pTky-2a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pTky-2a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07253).

Reference section:

Kato, K., Guo, J., Taguchi, F., Daimaru, O., Tajima, M., Haibara, H., Matsuda, J., Sumiya, M., Yogo, Y., Phylogenetic comparison between archetypal and disease-associated JC virus isolates in Japan. Jpn. J. Med. Sci. Biol., 47, 167-178 (1994). PMID 7823412. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07253_A9F3p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F ()
Sequence file: RDB07253_A9F3a.seq check
>07253_07253_A9F3_2_pUC-CAPbs_up_F_G04_19_ABI24.ab1
    1 TAACGGGGGC AGTGAGCGCA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG
   61 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT
  121 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTTG CATGCCTGCA GGTCGACTCT
  181 AGAGGATCCT GTGTTTTCAT CATCACTGGC AAACATTTCT TCATGGCAAA ACAGGTCTTC
  241 ATCCCACTTC TCATTAAATG TATTCCACCA GGATTCCCAC TCATCTGTTC CATAGGTTGG
  301 CACCTAAAAA CAAAAAATTC AGTTTATGTA AAAAACAAAA TGCCCTGCAA AAGAAAAATT
  361 GTGGTTTACC TTAAAGCTTT AGATCCCTGT AGGGAGTGTC TCCAAGAACC TTCTCCCAGC
  421 AATGAAGAGC TTCTTGGGTT AAGTCACACC CAAACCATTG TCTGAAGCAA TCAAAGCAAT
  481 AGCAATCTAT CCACACAAGT GGGCTGCTTC TTAAAAATTT TCTGTTTCTA TGCCTTAATT
  541 TTAGCATGCA CATTAAACAG GGGCAATGCA CTGAAGGATT AGTGGCACAA TTAGGCCATT
  601 CCTTGCAATA AAGAGTATCA GAATTAGGAG GAAAATCACA ACCAACCTCT GAACTATTCC
  661 ATGTACCAAA ATCAGGCTGA TGAGCAACTT TTACACCTTG TTCCATTTTT TTATATAAAA
  721 AGTTCATTCT CTTCATTTTG TCTTCGTCCC CACCTTTATC AGGGTGAAGT TCTTTGCATT
  781 TTTTCAGATA AGCTTTTCTC ATGACAGGAA TGTTTCCCCA TGCAGATCTA TCAAGGCCTA
  841 ATAAATCCAT GAGCTCCATG GATTCCTCCC TATTCAGCAC TTTGTCCATT TTTTGCTTTT
  901 TGTAGCAAAA AATTAGTGCA AAAAAGGGAA AAACAAGGGA ATTTCCCTGG GCCTCCTAAA
  961 AAGCCTCCAC GCCCTTACTA CTTCTGAGTA AGCTTGGGAG GCGGAGGCGG CCTCGGCCTC
 1021 CTGTATATAT AAAAAAAAGG AATGTAGGGA GGAGCTGGGC CTAAAACTGC ATGCCTGCAG
 1081 CCAAGCATGA AGCTTCATAC TTACGGAGAC TGATCAAGCC TGGACCAGTT CACCAAGGTG
1141 AAC
//
Primer: pUC-M13_-100 ()
Sequence file: RDB07253_A9F3b.seq check
>07253_07253_A9F3_2_pUC-M13_-100_H04_22_ABI24.ab1
    1 GGGGTCAGCG GATTAGTTGG GTACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC
   61 GGCCAGTGAA TTCGAGCTCG GTACCCGGGG ATCCCAACAC TCTACCCCAC CTAAGAAGAA
  121 AAAAAAGGTA GAAGACCCTA AAGACTTTCC TGTAGATCTG CATGCATTCC TCAGTCAAGC
  181 TGTGTTTAGT AATAGAACTG TTGCTTCTTT TGCTGTGTAT ACCACTAAAG AAAAAGCTCA
  241 AATTTTATAT AAGAAACTTA TGGAAAAATA TTCTGTAACC TTTATAAGTA GACATGGTTT
  301 TGGGGGTCAT AATATTTTGT TTTTTTTAAC ACCACATAGA CATAGAGTTT CAGCAATTAA
  361 TAATTACTGT CAGAAACTAT GTACCTTTAG TTTTTTAATT TGTAAAGGTG TGAATAAGGA
  421 ATACTTGTTT TACAGTGCCT TGTGTAGACA GCCATATGCA GTAGTGGAAG AAAGTATTCA
  481 GGGGGGCCTT AAGGAGCATG ACTTTAACCC AGAAGAACCA GAAGAAACTA AGCAGGTTTC
  541 ATGGAAATTA GTTACACAGT ATGCTTTGGA CACCAAGTGT GAGGATGTTT TCTTGCTTAT
  601 GGGCATGTAC TTAGACTTTC AGGAAAACCC ACTGCAATGC AAAAAATGTG AAAAGAAGGA
  661 TCAGCCAAAT CACTTTAACC ATCATGAAAA GCACTATTAT AATGCCCAAA TTTTTGCAGA
  721 TAGCAAAAAT CAAAAAAGCA TTTGCCAGCA GGCTGTTGAT ACTGTAGCAG CCAAACAAAG
  781 GGTTGACAGT CTCCATATGA CCAGAGAAGA AAATGTTAGT TGAAAGGTTT AATTTTTTGC
  841 TTGATAAAAT GGACCTTAAT TTTTGGGGGC ACATGGGCAA TGCTGTTTTA GAGCAATATA
  901 TGGCTGGGGT GGCCTGGATT CATTGTTTTG CTGCCTCAAA TGGACACAAG TCAAATATGA
  961 GTTCTTAAAA TGCATTGTAT TAAAACATTT CCAAAAAAAA GGTACTGGCT ATTCAAAGGG
 1021 GCCAAATAGA CAGTGGCAAA AACTACCTTT AGCTGCAGCC TTTACTTGGA TCTCTGTGGG
 1081 GGGAAAGTTC AATTAAATGG TAATAATGCC CATTTAAGAA AGATTAACTT TGAGTAGGAG
1141 GTGGGGGTTA ATAGAATCCA CTTCATATGG TGGTGATATG ACTGACGCAC G
//
Primer: JCsp_f1 ()
Sequence file: RDB07253_A9F3c.seq check
>07253_07253_A9F3_2_JCsp_f1_D01_10_ABI24.ab1
    1 GCCTTGAGCA TATGAGATGG CCCCAACAAA AAGAAAAGGA GAAAGGAAGG ACCCCGTGCA
   61 AGTTCCAAAA CTTCTTATAA GAGGAGGAGT AGAAGTTCTA GAAGTTAAAA CTGGGGTTGA
  121 CTCAATTACA GAGGTAGAAT GTTTTTTAAC TCCAGAAATG GGTGACCCAG ATGAGCATCT
  181 TAGGGGTTTT AGTAAGTCAA TTTCTATATC AGATACATTT GAAAGTGACT CCCCAAATAA
  241 GGACATGCTT CCTTGTTACA GTGTGGCCAG AATTCCACTA CCCAATCTAA ATGAGGATCT
  301 AACCTGTGGA AATATACTAA TGTGGGAGGC TGTGACCTTA AAAACTGAGG TTATAGGGGT
  361 AACAACTTTG ATGAATGTGC ACTCTAATGG TCAAGCAACT CATGACAATG GTGCAGGAAA
  421 GCCAGTGCAG GGCACCAGCT TTCATTTTTT TTCTGTTGGG GGGGAGGCTT TAGAATTACA
  481 GGGGGTGGTT TTTAACTACA GAACAAAGTA CCCAGATGGA ACAATTTTTC CAAAGAATGC
  541 AACAGTGCAA TCTCAAGTAA TGAACACAGA GCACAAGGCG TACCTAGATA AGAACAAAGC
  601 ATATCCTGTT GAATGTTGGG TTCCTGATCC CACCAGAAAT GAAAACACAA GATATTTTGG
  661 GACACTAACA GGAGGAGAAA ATGTTCCTCC AGTTCTTCAT ATAACAAACA CTGCCACAAC
  721 AGTGCTGCTT GATGAATTTG GTGTTGGGCC ACTTTGCAAA GGTGACAACT TGTATTTGTC
  781 AGCTGTGGAT GTTTGTGGCA TGTTTACTAA CAGATCTGGT TACCAGCAGT GGAGAGGACT
  841 GTCCAGATAT TTTAAGGTTC AGCTAAGAAA AAGGAGGGTT AAAAACCCCT ACCCAATTTC
  901 TTTCCTTCTT ACTGATTTAA TTAACAGAAG GACCCCTAGA GTTGATGGGC AGCCTATGTA
  961 TGGCATGGAT GCTCAGGTAG AGGAGGTTAG AGTTTTTTGA GGGGACAGAG GAAACTTCCA
 1021 GGGGACCCAG ACATGATGAG ATATGTTGAC AGATATGGGA CAGTTTGCAG ACCAAGATGC
 1081 TTGTTAATCA AAAGCCCGTT TAATGGTATA TGGCAGGTAC ATTTTCATAA GTATATCAGC
1141 TTACTTGATC AGTGGCCAAG GTTTAACCT
//
Primer: JCsp_R2 ()
Sequence file: RDB07253_A9F3d.seq check
>07253_07253_A9F3_2_JCsp_R2_D03_12_ABI24.ab1
    1 CCGTCCAGTA AGCTGGTTAT ACTTTATTAA AATGTACTGC ATATTACAAT AAAGGCTTTT
   61 GATTACAGCA TCTTTGTCTG CAACTGTCCA TATCTGTCAA CATATCTCAT CATGTCTGGG
  121 TCCCCTGGAA GTTCCTCTGT CCCCTCAAAA ACTCTAACCT CCTCTACCTG AGCATCCATG
  181 CCATACATAG GCTGCCCATC AACTCTAGGG GTCCTTCTGT TAATTAAATC AGTAAGAAGG
  241 AAAGAAATTG GGTAGGGGTT TTTAACCCTC CTTTTTCTTA GCTGAACCTT AAAATATCTG
  301 GACAGTCCTC TCCACTGCTG GTAACCAGAT CTGTTAGTAA ACATGCCACA AACATCCACA
  361 GCTGACAAAT ACAAGTTGTC ACCTTTGCAA AGTGGCCCAA CACCAAATTC ATCAAGCAGC
  421 ACTGTTGTGG CAGTGTTTGT TATATGAAGA ACTGGAGGAA CATTTTCTCC TCCTGTTAGT
  481 GTCCCAAAAT ATCTTGTGTT TTCATTTCTG GTGGGATCAG GAACCCAACA TTCAACAGGA
  541 TATGCTTTGT TCTTATCTAG GTACGCCTTG TGCTCTGTGT TCATTACTTG AGATTGCACT
  601 GTTGCATTCT TTGGAAAAAT TGTTCCATCT GGGTACTTTG TTCTGTAGTT AAAAACCACC
  661 CCCTGTAATT CTAAAGCCTC CCCCCCAACA GAAAAAAAAT GAAAGCTGGT GCCCTGCACT
  721 GGCTTTCCTG CACCATTGTC ATGAGTTGCT TGACCATTAG AGTGCACATT CATCAAAGTT
  781 GTTACCCCTA TAACCTCAGT TTTTAAGGTC ACAGCCTCCC ACATTAGTAT ATTTCCACAG
  841 GTTAGATCCT CATTTAGATT GGGTAGTGGA ATTCTGGCCA CACTGTAACA AGGAAGCATG
  901 TCCTTATTTG GGGAGTCACT TTCAAATGTA TCTGATATAG AAATTGACTT ACTAAAACCC
  961 CTAAGATGCT CATCTGGGTC ACCCATTTCT GGGAGTTTAA AAAAACATTT CTACCTCTGT
 1021 AAATTTGAGT CAACCCCAGT TTTAACTTCT AGAACTTCTA CTCTTCTTCT ATAGAGTTTT
1081 GGAACTGCAC GGATCGTCGT TTCTCATTTT CGTTTTTGAT TGGGGGCA
//
Primer: JCsp_f4 ()
Sequence file: RDB07253_A9F3e.seq check
>07253_07253_A9F3_2_JCsp_f4_H01_22_ABI24.ab1
    1 GCCTCATGGG ATTCTCCTAT TCAGCACTTT GTCCATTTTT GCTTTTTGTA GCAAAAAATT
   61 AGTGCAAAAA AGGGAAAAAC AAGGGAATTT CCCTGGCCTC CTAAAAAGCC TCCACGCCCT
  121 TACTACTTCT GAGTAAGCTT GGAGGCGGAG GCGGCCTCGG CCTCCTGTAT ATATAAAAAA
  181 AAGGGAAGGT AGGGAGGAGC TGGCTAAAAC TGGATGGCTG CCAGCCAAGC ATGAGCTCAT
  241 ACCTAGGGAG CCAACCAGCT GACAGTCACA AGTAAACAAA GCACAAGGCT AAAACTGGAT
  301 GGCTGCCAGC CAAGCATGAG CTCATACCTA GGGAGCCAAC CAGCTGACAG TCACAAGTAA
  361 ACAAAGCACA AGGGGAAGTG GAAAGCAGCC AGGGGAACAT GTTTTGCGAG CCAGAGCTGT
  421 TTTGGCTTGT CACCAGCTGG CCATGGTTCT TCGCCAGCTG TCACGTAAGG CTTCTGTGAA
  481 AGTTAGTAAA ACCTGGAGTG GAACTAAAAA AAGAGCTCAA AGGATTTTAA TTTTTTTGTT
  541 AGAATTTTTG CTGGACTTTT GCACAGGTGA AGACAGTGTA GACGGGAAAA AAAGACAGAA
  601 ACACAGTGGT TTGACTGAGC AGAGATACAG TGCTTTGCCT GAACCAAAAG CTACATAGGT
  661 AAGTAATGTT TTTTTTTGTG TTTTCAGGTT CATGGGTGCC GCACTTGCAC TTTTGGGGGA
  721 CCTAGTTGCT ACTGTTTCTG AGGCTGCTGC TGCCACAGGA TTTTCAGTAG CTGAAATTGC
  781 TGCTGGAGAG GCTGCTGCTA CTATAGAAGT TGAAATTGCA TCCCTTGCTA CTGTAGAGGG
  841 GATTACAAGT ACCTCTGAGG CTATAGCTGC TATAGGCCTT ACTCCTGAAA CATATGCTGT
  901 AATTACTGGA GCTCCGGGGG CTGTAGCTGG GTTTGCTGCA TTGGTTCAAA CTGTAACTGG
  961 TGGTAGTGCT ATTGCTCAGT TGGGATATAG ATTTTTTTGC TGACTGGGAT CATAAAGTTT
 1021 CAAACAGTTT GGGCTTTTTT CAGCAGCCAG CTATGGCTTT ACAGTTATTT AATCAGAGAC
 1081 TACTAATGGA AATTTTTAAT TTGCTGGAGT GATTGCTTTG TTACCATATT CAACTTAATT
1141 TTAGAATTCG CCTTAGAAAC C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kato, K., Phylogenetic comparison between archetypal and disease-associated JC virus isolates in Japan. Jpn. J. Med. Sci. Biol., 47, 167-178 (1994). PMID 7823412. [link to RRC of NBRP]