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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMT/cl 108 (#RDB07257)

Complete genome of BK virus, lineage Ic

Clone info. Complete genome of BK virus, lineage Ic. BKV genomic DNA derived from the urine of a Japanese systemic lupus erythematosus patient.
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r
Gene/insert name Hum_Polyoma BKV genomic DNA
Reference of insert sequence AB485711 (DDBJ accession number)
Depositor|Developer Yogo, Yoshiaki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Virol., 63, 3195-3199 (1989)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Virol., 63, 3195-3199 (1989))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07257 pMT/cl 108 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMT/cl 108 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07257).

Reference section:

Sugimoto, C., Hara, K., Taguchi, F., Yogo, Y., Growth efficiency of naturally occurring BK virus variants in vivo and in vitro. J. Virol., 63, 3195-3199 (1989). PMID 2542627. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07257_A3Kj.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB07257_A3Kja.seq check
>07257_07257_A3Kj_2_Amp_R_H11_23.ab1
    1 CTTCGTTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
  181 CGAGGCCCTT TCGTCTTCAA GAATTCTCAT GTTTGACAGC TTATCATCGA TAAGCTTTAA
  241 TGCGGTAGTT TATCACAGTT AAATTGCTAA CGCAGTCAGG CACCGTGTAT GAAATCTAAC
  301 AATGCGCTCA TCGTCATCCT CGGCACCGTC ACCCTGGATG CTGTAGGCAT AGGCTTGGTT
  361 ATGCCGGTAC TGCCGGGCCT CTTGCGGGAT ATCGTCCATT CCGACAGCAT CGCCAGTCAC
  421 TATGGCGTGC TGCTAGCGCT ATATGCGTTG ATGCAATTTC TATGCGCACC CGTTCTCGGA
  481 GCACTGTCCG ACCGCTTTGG CCGCCGCCCA GTCCTGCTCG CTTCGCTACT TGGAGCCACT
  541 ATCGACTACG CGATCATGGC GACCACACCC GTCCTGTGGA TCCAGATGAA AACCTTAGGG
  601 GCTTTAGTCT AAAGCTAAGT GCTGAAAATG ACTTTAGCAG TGATAGCCCA GAGAGAAAAA
  661 TGCTTCCCTG TTACAGCACA GCAAGAATTC CCCTCCCCAA TTTGAATGAG GACCTAACCT
  721 GTGGAAATCT CCTGATGTGG GAGGCTGTAA CTGTACAAAC AGAGGTTATT GGAATAACTA
  781 GCATGCTTAA CCTTCATGCA GGGTCACAAA AAGTACATGA GCATGGTGGA GGGAAGCCTA
  841 TTCAAGGCAG TAATTTCCAC TTCTTTGCTG TTGGTGGAGA CCCCTTGGAA ATGCAGGGAG
  901 TGCTAATGAA TTACAGGACA AAGTACCCAG AAGGTACTAT AACTCCAAAA AACCCAACAG
  961 CCCAGTCCCA GGTAATGAAT ACTGACCATA AAGGCCTATT TGGACAAAAC AATGCTTATC
 1021 CAGTTGAGTG CTGGATTCCT GATCCCAGTA GAAATGAAAT ACTAGGTATT TGGGACTTTG
 1081 ACAGGAGGGA AAATGTCCCC AGTACTCATG TGACACACAG CTACACAGTG TTGCTAGATG
1141 ACAGGTGGTG GGCTCATGTA GCTGATAGCT GTATTGTTTC AGCTGCCTGG TA
//
Primer:
Sequence file: RDB07257_A3Kjb.seq check
>07257_07257_A3Kj_pBR322_BamHI_R_G08_20.ab1
    1 CCTAAAGTTA GGAGCAATTC TACCTCTGTT ATAGCATCTA CCCCAGTTTT AACTTCTAGA
   61 ACTTCTACTC CTCCTTTTAT TAGTAGTTTT GGCACTTGCA CGGGGTCCTT TGGCTTTTTG
  121 GGAGCTGCCC CTGGACACTC TCCTTTTCTT TTGGTTGGGG CCATCTTCAT ATGCTTCAAG
  181 AGCAGGTGTT ACAGTCCCGT ACAGGCCTAG AAGTAAAGGC AACATCCATT GAGGAGCAGT
  241 TCTTTGATTA GCACCTCCTG GGGCAATAGT TTTTTCTATA AACTCTCCTG AATGTATACT
  301 TTGTTGATTT CTTAAGTCCA TTCTTTGTGT AACTTCTTCT ATACTGTCAG CATCATCTAT
  361 ACTATAAGTA TGGCCAAAAT GTACACGGGT ACCTTCCCTT TCAGCTACTT GTCTAACCAT
  421 TGAGGGCCTA ATAGGGGAAA GATCAGAATA ATATTCCTGA ATATAATTAT AAAAGTTTAT
  481 AGGGGCATTT ACAATTGTCC AGGTAGTTTC CTCCAAAAAT CTAGCCAAGG AGTCTCTAAA
  541 AAATCTTTCT GTTCTTCTTT GTAATTCTTG TGAGGTTATA GCAGGTATAT CATCCCTAAT
  601 AACATGCCAC AAAGCCTGGG AAATAGTAGC AAACAAAGAA GGACCCCAAT GCCTAGGATC
  661 AAGGTATTGA ATATTATTTA CAAAAGTATT TACACCAGGA AACAAAATAT CATAGTACTC
  721 ATCTGGGTTA AACAATTCCA AAGCCATGCC TGATTGCTGA TAGAGGCCTA CAGTGGAAAC
  781 TTTGTGATCC CAATCACTAA AGAACCTATA CCCTACTTGA GCCAAGGAAC TAACACCAGT
  841 AACAGTTTGA ATTAAAGCAG CAAACCCAGC AATAGCCCCA GGAGCACCAG CAATTACAGC
  901 ATATGTTTGA GGAGTTAGGC CTATAGCAGC TATAGCCTCT GAGGTACTTG TTATGCCCTC
  961 TACAGTAGCA AGGGATGCAA TTTGAACTTC TATAGCAGCA GCAGCCTCCC CAGCAGCAAT
 1021 TTCAGCCACT GAAAATCTGT GGCAGGCAGC AGCCTCAGAT TACACTGGCA ACTAGGTCCC
 1081 CCAAAAGTTG CTAGAGCAGC ACCATGACTT GGAAATACCA AAAAAAGGAA TACTACCTTA
 1141 GAGTTCTTTT ACGAGTTCTT TTAACAGGCA AGTAGCATGG TACTTTTGGT TTTTTCCGTA
1201 TCTCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sugimoto, C., Growth efficiency of naturally occurring BK virus variants in vivo and in vitro. J. Virol., 63, 3195-3199 (1989). PMID 2542627. [link to RRC of NBRP]