Please review the QC test results indicated by icon below as well as clone information before placing your order.

pKOM-5 (#RDB07264)

Complete genome of BK virus, lineage Ib 1

Clone info.	Complete genome of BK virus, lineage Ib 1. BKV genomic DNA derived from the urine of a Japanese bone marrow transplant patient.
Vector backbone	pUC19 (Plasmid)
Size of vector backbone	2,690 bp
Selectable markers	Amp^r
Gene/insert name	Hum_Polyoma BKV genomic DNA
Reference of insert sequence	AB211374 (DDBJ accession number)
Depositor|Developer	Yogo, Yoshiaki |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Mol. Evol., 63, 341-352 (2006))

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07264	pKOM-5	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pKOM-5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07264).

Reference section:

Nishimoto, Y., Takasaka, T., Hasegawa, M., Zheng, H.Y., Chen, Q., Sugimoto, C., Kitamura, T., Yogo, Y., Evolution of BK virus based on complete genome data. J. Mol. Evol., 63, 341-352 (2006). PMID 16897259. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07264_A8F5p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100) Region: insert 5' Sequence file: RDB07264_A8F5a.seq
>07264_07264_A8F5_1_Reverse2_E02_14_ABI24.ab1     1 ATAACGCATT ACGCCAAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC CCCCATTTCT    61 GGGTTTAGGA AGCATTCTAC CTCTGTTATA GCATCTACCC CAGTTTTAAC TTCTAGAACT   121 TCTACTCCTC CTTTTATTAG TAGTTTTGGC ACTTGCACGG GTTCCTTTGG CTTTTTGGGA   181 GCTGCCCCTG GACACTCTCC TTTTCTTTTG GTTGGGGCCA TCTTCATATG CTTCAAGAGC   241 AGGTGTTACA GTCCCGTACA GGCCTAGAAG TAAAGGCAAC ATCCATTGAG GAGCAGTTCT   301 TTGATTAGCA CCTCCTGGGG CAATAGTTTT TTCTATAAAC TCTCCTGAAT GTACAGTTTG   361 TTGATTTCTT AAGTCCATTC TTTGTGTAAC TTCTTCTATA CTGTCAGCAT CATCTATACT   421 ATAAGTATGG CCAAAATGTA CACGGGTACC TTCCCTTTCA GCCACTTGTC TAACCATTGA   481 GGGCCTAATA GGGGAAAGAT CAGAATAATA TTCTTGAATA TAATTATAAA AGTTTATAGG   541 GGCATTTACA ATTGTCCAGG TAGTTTCCTC CAAAAATCTA GCCAAGGAGT CTCTAAAAAA   601 TCTTTCTGTT CTTCTTTGCA ATTCTTGTGA GGTTATAGCA GGTATATCAT CCCTAATAAC   661 ATGCCACAAA GCCTGGGAAA TAGTAGCAAA CAAGGAAGGA CCCCAATGCC TAGGATCAAG   721 GTATTGAATA TTATTTACAA AAGTATTTAC ACCAGGAAAC AAAATATCAT AGTACTCATC   781 TGGGTTAAAC AATTCCAAAG CCATGCCTGA TTGCTGATAG AGGCCTACAG TGGAAACTTT   841 GTGATCCCAA TCACTAAAAA AACCTATACC CTACTTGAGC CAAGGAACTA ATACCAGTAA   901 CAGTTTTGAA TTAAAGCAGC AAACCCAGCA ATAGCCCCAG GAGCACCAGC AATTACAGCA   961 TATGTTTGAG GAGTTAGGCC TATAGCAGCT ATAGCCTCTG AGGTACTTTG TATGCCCTCT  1021 ACAGTAGCAA CGGAATGCCA TTTTGAAACC TTCTATAGGC AGGCAGGCAG GTCCGTCCCT 1081 CAAGGTCAGT CAAATC //
Primer: M13_-40 (Pr0410) Region: insert 3' Sequence file: RDB07264_A8F5b.seq
>07264_07264_A8F5_1_20_M13_-40_G01_07_ABI08.ab1     1 AAAGCAAGGG ATTCGAGGCT CGGTACCCGG GGATCCAGAT GAAAACCTTA GGGGCTTTAG    61 TCTAAAGCTA AGTGCTGAAA ATGACTTTAG CAGTGATAGC CCAGAAAGAA AAATGCTTCC   121 CTGTTACAGC ACAGCAAGAA TTCCCCTCCC CAATTTAAAT GAGGACCTAA CCTGTGGAAA   181 TCTACTGATG TGGGAGGCTG TAACTGTACA AACAGAGGTC ATTGGAATAA CTAGCATGCT   241 TAACCTTCAT GCAGGGTCAC AAAAAGTGCA TGAGCATGGT GGAGGTAAAC CTATTCAAGG   301 CAGTAATTTC CACTTCTTTG CTGTTGGTGG AGACCCCTTG GAAATGCAGG GAGTGCTAAT   361 GAATTACAGG ACCAAGTACC CAGATGGTAC TATAACCCCA AAAAACCCAA CAGCCCAGTC   421 CCAGGTAATG AATACTGACC ATAAGGCCTA TTTGGACAAA AACAATGCTT ATCCAGTTGA   481 GTGCTGGGTT CCTGATCCTA GTAGAAATGA AAATACTAGG TATTTTGGGA CTTTCACAGG   541 AGGGGAAAAT GTTCCCCCAG TACTTCATGT GACCAACACA GCTACCACAG TGTTGCTAGA   601 TGAACAGGGT GTGGGGCCTC TTTGTAAAGC TGATAGCCTG TATGTTTCAG CTGCTGATAT   661 TTGTGGCCTG TTTACTAACA GCTCTGGAAC ACAACAGTGG AGAGGCCTTG CAAGATATTT   721 TAAGATTCGC CTGAGAAAAA GATCTGTAAA AAATCCTTAC CCAATTTCCT TTTTGCTAAG   781 TGACCTTATA AACAGGAGAA CCCAGAGAGT GGATGGGCAG CCTATGTATG GTATGGAATC   841 CCAGGTAGAA GAGGTTAGGG TGTTTGATGG CACAGAAAGA CTTCCAGGGG ACCCAGATAT   901 GATAAGATAT ATTGATAAAC AAGGACAATT GCAAACCAAA ATGCTTTAAA CAGGGTGCTT   961 TTATTGTACA TATACATTTA ATAAATGCTG CTTTTGTATA AGCCACTTTT TAAGCTTTGT  1021 GTTATTTTGG GGGTGGGTGT TTTAGGCCTT TTAAAACATT GAAAGCCTTT ACACAAATGC  1081 AACTCTTGAC TATGGGGATC TACTTGGCAT CTTCAGCCAG GGGCTGAAGG TAATTTCTTG 1141 GA //
Primer: Bkvirus_F1 (Pr0564) Region: insert mid Sequence file: RDB07264_A8F5c.seq
>07264_07264_A8F5_1_BKvirus_F1_C03_09_ABI24.ab1     1 CCATTCTGAT CTTCTTCTCT TGTGATATCA AGAATACATT TCCCCATGCA TATATTATAT    61 TTCATCCTTG AAAAAGTATA CATACTTATC TCAGAATCCA GCCTTTCCTT CCATTCAACA   121 ATTCTAGATT GTATATCTGT TGCAAAATCA GCTACAGGCC TAAACCAAAT TAGCAGTAGC   181 AACAAGGTCA TTCCACTTTG TAAAATTCTT TTTTCAAGTA AGAACTCTGA GTTTTGTAAG   241 GATTTTCTTA AATATATTTT GGGTCTAAAA TCTATCTGTC TTACAAATCT AGCCTGCAGG   301 GTTTTAGGAA CAGGATACTC ATTCATTGTA ACCAGGCCTG GTGGAAATAT TTGGGTTCTT   361 TTGTTTAAAT GTTTCTTTTC TAAATTAACC TTAACACTTC CATCTAAATA ATCTCTCAAA   421 CTGTCTAAAT TGTTTATTCC ATGTCCTGAA GGCAAATCCT TTGATTCAGC CCCTGTTCCT   481 TTTACATCTT CAAAAACAAC CATGTACTGA TCTATAGCTA CACCTAGTTC AAAGGTTAGC   541 CTTTCCATGG GTAGGTTTAC ATTTAAGGCT TTACCTCCAC ACAAATCTAA TAACCCTGCA   601 GCTAGTGTTG TTTTTCCACT ATCAATGGGA CCTTTAAATA ACCAGTATCT TCTTTTAGGT   661 ACATTAAAAA CAATACAGTG CAAAAAATCA AATATAACAG AATCCATTTT AGGTAGCAAA   721 CAGTGCAGCC AAGCAACACC TGCCATATAT TGTTCCAGTA CAGCATTTCC ATGAGCTCCA   781 AATATTAAAT CCATTTTATC TAATATATGA TTAAATCTTT CTGTTAGCAT TTCTTCTCTG   841 GTCATATGAA GGGTATCTAC TCTTTTTTTT TAGCTAAAAA CTGTATCTAC TGCTTGCTGA   901 CAAATACTTT TTTTGATTTT TTACTTTCTG CAAAAAATAG TAGCATTTTG CAAAATGCTT   961 TTTCATGATA CTTAAAGTGA TAAAGGTTGG TCTTTTTTTT TCTGACACTT CTTTACACTC  1021 CTTCCTACCA TTGTATTGAA GTTCTAAAAT ACCATAGCGG ATTAGTAAAA ACACATCGTC 1081 CAACACCTTG //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Nishimoto, Y., Evolution of BK virus based on complete genome data. J. Mol. Evol., 63, 341-352 (2006). PMID 16897259. [link to RRC of NBRP]

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External Database
Hum_Polyoma BKV

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