Please review the QC test results indicated by icon below as well as clone information before placing your order.

pTHK-9 (#RDB07265)

Complete genome of BK virus, lineage Ic

Clone info.	Complete genome of BK virus, lineage Ic. BKV genomic DNA derived from the urine of a Japanese renal transplant patient.
Vector backbone	pUC19 (Plasmid)
Size of vector backbone	2,690 bp
Selectable markers	Amp^r
Gene/insert name	Hum_Polyoma BKV genomic DNA
Reference of insert sequence	AB211379 (DDBJ accession number)
Depositor|Developer	Yogo, Yoshiaki |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Mol. Evol., 63, 341-352 (2006))

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07265	pTHK-9	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pTHK-9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07265).

Reference section:

Nishimoto, Y., Takasaka, T., Hasegawa, M., Zheng, H.Y., Chen, Q., Sugimoto, C., Kitamura, T., Yogo, Y., Evolution of BK virus based on complete genome data. J. Mol. Evol., 63, 341-352 (2006). PMID 16897259. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07265_A8F5p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100) Region: insert 5' Sequence file: RDB07265_A8F5a.seq
>07265_07265_A8F5_3_Reverse2_G02_20_ABI24.ab1     1 AAAAACAGCA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCCATTT    61 CTGGGTTTAG GAAGCATTCT ACCTCTGTTA TAGCATCTAC CCCAGTTTTA ACTTCTAGAA   121 CTTCTACTCC TCCTTTTATT AGTAGTTTTG GCACTTGCAC GGGGTCCTTT GGCTTTTTGG   181 GAGCTGCCCC TGGACACTCT CCTTTTCTTT TGGTTGGGGC CATCTTCATA TGCTTCAAGA   241 GCAGGTGTTA CAGTCCCGTA CAGGCCTAGA AGTAAAGGCA ACATCCATTG AGGAGCAGTT   301 CTTTGATTAG CACCTCCTGG GGCAATAGTT TTTTCTATAA ACTCTCCTGA ATGTACACTT   361 TGTTGATTTC TTAAGTCCAT TCTTTGTGTA ACTTCTTCTA TACTGTCAGC ATCATCTATA   421 CTATAAGTAT GGCCAAAATG TACACGGGTA CCTTCCCTTT CAGCTACTTG TCTAACCATT   481 GAGGGCCTAA TAGGGGAAAG ATCAGAATAA TATTCCTGAA TATAATTATA AAAGTTTATA   541 GGGGCATTTA CAATTGTCCA GGTAGTTTCC TCCAAAAATC TAGCCAAGGA GTCTCTAAAA   601 AATCTTTCTG TTCTTCTTTG TAATTCTTGT GAGGTTATAG CAGGTATATC ATCCCTAATA   661 ACATGCCACA AAGCCTGGGA AATAGTAGCA AACAAAGAAG GACCCCAATG CCTAGGATCA   721 AGGTATTGAA TATTATTTAC AAAAGTATTT ACACCAGGAA ACAAAATATC ATAGTACTCA   781 TCTGGGTTAA ACAATTCCAA AGCCATGCCT GATTGCTGAT AGAGGCCTAC AGTGGAAACT   841 TTGTGATCCC AATCACTAAA GAACCTATAC CCTACTTGAG CCAAGGAACT AACACCAGTA   901 ACAGTTTGAA TTAAAGCAGC AAACCCAGCA ATAGCCCCCA GGAGCACCAG CAATTACAGC   961 ATATGTTTGA GGGAGTTAGG CCTATAGCAG CTATAGCCCT CTGAGGTACT TTGATATGCC  1021 CTCTACAGTA GCAAGGAATG CAATTTGGAC CTTTCTATAG CAGCAGCAGT CCTTCGCCAA 1081 GGCCAGCCAA TTCCAGAC //
Primer: M13_-40 (Pr0410) Region: insert 3' Sequence file: RDB07265_A8F5b.seq
>07265_07265_A8F5_3_M13_-40_H02_23_ABI24.ab1     1 CAGGCGGTAA TCGGAGCTCG GTACCGGGGA TCCAGATGAA AACCTTAGGG GCTTTAGTCT    61 AAAGCTAAGT GCTGAAAATG ACTTTAGCAG TGATAGCCCT GAGAGAAAAA TGCTTCCCTG   121 TTACAGCACA GCAAGAATTC CCCTCCCCAA TTTGAATGAG GACCTAACCT GTGGAAATCT   181 CCTGATGTGG GAGGCTGTAA CTGTACAAAC AGAGGTTATT GGAATAACTA GCATGCTTAA   241 CCTTCATGCA GGGTCACAAA AAGTACATGA GCATGGTGGA GGGAAGCCTA TTCAAGGCAG   301 TAATTTCCAC TTCTTTGCTG TTGGTGGAGA CCCCTTGGAA ATGCAGGGAG TGCTAATGAA   361 TTACAGGACA AAGTACCCAG AAGGTACTAT AACTCCAAAA AACCCAACAG CCCAGTCCCA   421 GGTAATGAAT ACTGACCATA AGGCCTATTT GGACAAAAAC AATGCTTATC CAGTTGAGTG   481 CTGGATTCCT GATCCCAGTA GAAATGAAAA TACTAGGTAT TTTGGGACTT TGACAGGAGG   541 GGAAAATGTT CCCCCAGTAC TTCATGTGAC CAACACAGCT ACCACAGTGT TGCTAGATGA   601 ACAGGGTGTG GGGCCTCTTT GTAAAGCTGA TAGCCTGTAT GTTTCAGCTG CTGATATTTG   661 TGGCCTGTTT ACTAACAGCT CTGGAACACA ACAGTGGAGA GGCCTTGCAA GATATTTTAA   721 GATTCGCCTG AGAAAAAGAT CTGTAAAGAA TCCTTACCCA ATTTCCTTTT TGCTGAGTGA   781 CCTTATAAAC AGGAGAACCC AGAGAGTGGA TGGGCAGCCT ATGTATGGTA TGGAATCCCA   841 GGTAGAAGAG GTCAGGGTGT TTGATGGCAC AGAAAAAACT TCCAGGGGGA CCCAGATATG   901 ATAAGATATA TTGACAAACA AGGACAATTG CAAACCAAAA TGCTTTTAAA CAGGTGCTTT   961 TTATTGTACA TATACATTTT AAATAAATGC TGCTTTTTGT ATAAGCCCAC TTTTTAAGCT  1021 TTGTGGTTAT TTTTGGGGGG TGGTTGGTTT TTAGTCCGTT TTAAAACACT GGAAGCGATT 1081 TACAACAAAT GCAACCTCTT TTG //
Primer: Bkvirus_F1 (Pr0564) Region: insert mid Sequence file: RDB07265_A8F5c.seq
>07265_07265_A8F5_3_BKvirus_F1_D03_12_ABI24.ab1     1 TCTGTTTTCT GAATCTTTCT TCTCTTGTGA TATCAAGAAT ACATTTCCCC ATGCATATAT    61 TATATTTCAT CCTTGAAAAA GTATACATAC TTATCTCAGA ATCCAGCCTT TCCTTCCATT   121 CAACAATTCT AGATTGTATA TCTGTTGCAA AATCAGCTAC AGGCCTAAAC CAAATTAGCA   181 GTAGCAATAA GGTCATTCCA CTTTGTAAGA TTCTTTTTTC AAGTAAAAAC TCTGAGTTTT   241 GTAAGGATTT TCTTAAATAT ATTTTGGGCC TAAAATCTAT CTGTCTTACA AATCTAGCCT   301 GCAGGGTTTT AGGGACAGGA TACTCATTCA TTGTAACCAA GCCTGGAGGA AATATTTGGG   361 TTCTTTTGTT TAAATGTTTC TTTTCTAAAT TAACCTTAAC ACTTCCATCT AAATAATCTC   421 TGAGACTGTC TAAATTGTTT ATTCCATGTC CTGAAGGCAA ATCCTTTGAT TCAGCCCCTG   481 TCCCTTTTAC ATCTTCAAAA ACAACCATGT ACTGATCTAT AGCTACACCT AGTTCAAAGG   541 TTAGCCTTTC CATGGGTAGG TTTACATTTA AGGCTTTACC TCCACACAAA TCTAATAACC   601 CTGCAGCTAG TGTTGTTTTT CCACTATCAA TAGGACCTTT AAATAACCAG TATCTTCTTT   661 TAGGTACATT AAAAACAATA CAGTGCAAAA AATCAAATAT AACAGAATCC ATTTTAGGTA   721 GCAAACAGTG CAGCCAAGCA ACACCTGCCA TATATTGTTC TAGTACAGCA TTTCCATGAG   781 CTCCAAATAT TAAATCCATT TTATCTAATA TATGATTAAA TCTTTCTGTT AGCATTTCTT   841 CTCTGGTCAT ATGAAGGGGT ATCTACTCTT TTTTTTAGCT AAAACTGTAT CTACTGCTTG   901 CTGACAAATA CTTTTTTGAT TTTTTACTTT TCTGCAAAGA TAGTAGCATT TTGCAAAAAT   961 GCTTTTCATG ATACTTAAAG TGATAAAGGT TGGTTCTTTT TTTCTGACAC TTTTTTTACA  1021 CTCGTCTACA TTGTTATTGA AACTTCCTAA TACATACCTA ATAAATTAAA ACACATTCCG 1081 TTCACCACCT TTTGTTCCTC TTACTTGGCC AT //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Nishimoto, Y., Evolution of BK virus based on complete genome data. J. Mol. Evol., 63, 341-352 (2006). PMID 16897259. [link to RRC of NBRP]

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External Database
Hum_Polyoma BKV

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