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pKOM-3 (#RDB07266)

Partial genome of BK virus, lineage III

Clone info. Partial genome of BK virus, lineage III. BKV genomic DNA derived from the urine of a Japanese bone marrow transplant patient. This clone contains a genomic fragment corresponding to the 3952nt - 5132nt and 1nt -1618nt of AB211386.1.
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2,690 bp
Selectable markers Amp^r
Gene/insert name Hum_Polyoma BKV genomic DNA
Reference of insert sequence AB211386 (DDBJ accession number)
Depositor|Developer Yogo, Yoshiaki |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J. Mol. Evol., 63, 341-352 (2006))
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Mol. Evol., 63, 341-352 (2006))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07266 pKOM-3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKOM-3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07266).

Reference section:

Nishimoto, Y., Takasaka, T., Hasegawa, M., Zheng, H.Y., Chen, Q., Sugimoto, C., Kitamura, T., Yogo, Y., Evolution of BK virus based on complete genome data. J. Mol. Evol., 63, 341-352 (2006). PMID 16897259. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07266_A8F5p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100)
Region: insert 5'
Sequence file: RDB07266_A8F5a.seq check
>07266_07266_A8F5_3_Reverse2_A09_03_ABI24.ab1
    1 ATAAAAGGAT TTCGCCAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC CCTAGTTAAG
   61 GCACTGTATA GTAAGTATTC CTTATTAACA CCCTTACAAA TTAAAAAACT AAAAGTACAC
  121 AGCTTTTGAC AGAAATTATT AATTGCAGAA ACTCTATGTC TATGTGGAGT TAAAAAGAAT
  181 ATAATATTAT GACCAGCACA CATGTGTCTA CTGATAAAAG TTACAGAATA TTTTTCCATA
  241 AGTTTTTTAT ACAGAATTTG AGCTTTTTCT TTAGTGGTAT ACACAGCAAA ACAGGCAAGT
  301 GTTCTATTAC TAAATACAGC TTGACTAAGA AACTGGTGTA GATCAGAGGG AAAGTCTTTA
  361 GGGTCTTCTA CCTTTCTTTT TTTTTTGGGT GGTGTTGAGT GTTGGGAATC TGCTGTTGCT
  421 TCTTCATCAC TGGCAAACAT ATCCTCATGG CAGAATAAAT CTTCATCCCA TTTTTCATTA
  481 AAGGAGCTCC ACCAGGACTC CCACTCTTCT GTTCCATAGG TTGGCACCTA TAAAAAAAAA
  541 ATAATTACTT AGGGTCTTTC TTTTAATTTA CTACTTTTCT AAATATAAAT TAGTTACCTT
  601 AAAGCTTTAG ATCTCTGAAG GGAGTTTCTC CAATTATTTG GACCCACCAT TGCAGGGTTT
  661 CTTCAGTGAG GTCTAAGCCA AACCACTGTG TGAAGCAATC AATGCAGTAG CAATCTATCC
  721 AAACCAATGG CTCTTTTCTT AAAAATTTTC TATTTAAATG CCTTAATCTT AGCTGACATA
  781 GCATGCAAGG GCAATGCACT GAAGGCTTTT TGGAACAAAT AGGCCATTCC TTGCAGTACA
  841 AAGTATCTGG GCAAAGAGGA AAATCAGCAC AAACCTCTGA GCTATTCCAG GTTCCAAAAT
  901 CAGGCTGATG AGCTACCTTT ACATCCTGCT CCATTTTTTT TATATAAAGT ATTCATTCTC
  961 TTCATTTTTA TCCTCGTCGC CCCCCTTTTG TCAGGGTGAA ATTCCCTTAC ACTTTTTTTT
 1021 AAATAGCTTT TTTCTCATTT AACGGCACGG TTTTCCTCAG GCAGCTCTTT CAAGACTTAA
1081 AGGATCCATG AGCTTCCCAA TG
//
Primer: M13_-40 (Pr0410)
Region: insert 3'
Sequence file: RDB07266_A8F5b.seq check
>07266_07266_A8F5_3_M13_-40_B09_06_ABI24.ab1
    1 CAGCAGTCAT CGAGCTCGGT ACCCGGGGAT CCCCCATTTC TGGGTTTAGA AAGCATTCTA
   61 CCTCTGTTAT AGCATCTACC CCAGTTTTAA CTTCTAGAAC TTCTACTCCT CCTTTTATTA
  121 GTAGTTTTGG CACTTGCACG GGTTCCTTTG GCTTTTTGGG AGCTGCCCCT GGACACTCTC
  181 CTTTTCTTTT GGTTGGGGCC ATCTTCATAT GCTTCAAGAG CAGGTGTTAC AGTCCCGTAC
  241 AGGCCTAGAA GCAAAGGCAA CATCCATTGA GGAGCAGTTC TTTGATTAGC ACCTCCTGGG
  301 GCAATAGTTT TTTCTATAAA CTCTCCTGAA TGTACACTTT CCTTATTTCT TAAATCCATT
  361 CTTTGGGTAA CTTCTTCTAT ACTGTCAGCA TTATCTATGC TGTAGGTATG GCCAAAATTT
  421 ACATGGGTAC CTTCCCTTTC AGCTACTTGC CTAACCATTG AAGGCCTAAT AGGGGACAAA
  481 TTAGAATAAT AATCCTGAAT ATAATTATAA AAGTTTATGG GGGCATTTAC AATTGTCCAG
  541 GTGGTTTCTT CCAAAAATCT AGCCAAAGAG TCCCTAAAAA ATCTCTCTGT TCTCCTTTGC
  601 AATTCTTGTG AAGTTATAGC AGGTATATCA TCTCTAATAA CATGCCACAA AGCCTGGGAA
  661 ATAGTAGCAA ACAAAGAAGG ACCCCAATGC CTAGGATCTA GATATTGAAT ATTATTAACA
  721 AAGGTATTTA CACCAGGAAA CAAAATATCA TAGTACTCAT CTGGGTTAAA CAATTCCAAA
  781 GCCATGCCTG ATTGCTGATA AAGGCCTACA GTGGAAACTT TGTGATCCCA ATCACTAAAA
  841 AAACCTATAC CCTACTTGAG CCAAAGAACT AATACCAGTA ACAGTTTGAA TTAAAGCAGC
  901 AAACCCAGCA ATAGCCCCCT GGAGCACCAG CAATTACAGC ATATGTTTTG AGGTGTTTAG
  961 GCCTATAGCA GCTATAGCCC TCTGAGGTAG TTGTTATGCC CTCTACAGTA GCACGGATGC
 1021 AATTTGAAAC TTCCTATGGG CAGCCAGCCA GTCGTCCCCA GCAGCAGTTT CAGGCCAACT
1081 GGATAAATCC TTGTTGGG
//
Primer: Bkvirus_R1 (Pr0566)
Region: insert mid
Sequence file: RDB07266_A8F5c.seq check
>07266_07266_A8F5_3_BKvirus_R1_C01_03_ABI08.ab1
    1 ATGGTTGCGG CCGGCAGCCT CAGGATACAC TGGGCAACTA GGTCCCCCAA AAGGTGCTAG
   61 AGCAGGCACC CATCAACCTG AAAATACAAA AAAAAAGCAT TACTTACCTA GGAGTCTTTT
  121 ACAGAGTCTT TTACAGCAGG TAAAGCAGTG GTACTTTTGT TTTTCCCGTC TACACTGTCT
  181 TCACCTCTAC AAAATTCCAG CAAAAGCTCT AAAATAAAAA TAAAAATCCT CTGAGCTCTT
  241 TTTTTAGTTC CAGTCCAGGT TTTACTAACT TTCACTGAAG CTTGTCGTGA CAGCTGGCGC
  301 AAAACCATGG CCTTTGTCCA GTATTAACTG GGGACAAGGC CAAGATTCTT AGGCTCGCAA
  361 AACATGTCTG TCTGGCTGCT TTCCACTCCT TTGGAAAGTT TCCACTTCCT CTTGTGTTTA
  421 TTTGAGAATG TTAGGGGCGG GGTTTCACTA TTAACTGCCA CTGGCTGGCT GCCCAGTCAT
  481 GCACTTGCTT CCTGAGGTCA TGGTTTGGCT GCATTCCATG GGAAAGCAGC TCCTCCCTGT
  541 GGCCTTTTTT TTTATAATAT ATAAGAGGCC GAGGCCGCCT CTGCCTCCAC CCTTTCTCTG
  601 AAGTAGTAAG GGTGTGGAGG CTTTTTCTGA GGCCTAGCAA AACTATTTGG GGAAATCCCT
  661 ATTGTTTTGC AATTTTCTGC AAAAATGGAT AAAGTCTTAA ACAGGGAAGA ATCCATGGAG
  721 CTCATGGACC TTTTAGGCCT TGAAAGAGCT GCCTGGGGAA ACCTTCCCTT AATGAGAAAA
  781 GCCTATTTAA AAAAAGTGTA AGGAATTTCA CCCTGACAAA GGGGGCGACG AGGATAAAAT
  841 GAAGAGAATG AATACTTTAT ATAAAAAAAT GGAGCAGGAT GTAAAGGTAG CTCATCAGCC
  901 TGATTTTGGA ACCTGGAAAT AGCTCAGAGG TTTGTGCTGA TTTTTCCTCT TTTGCCCAGG
  961 ATACTTTGTA ACTGCAAGGA AATGGCCTAT TTGTTCCCAA AAAGCCTTTC AGTGCATTGC
 1021 CCTTGCATGC TAATGTCAGC TAAGATTTAA GGGCATTTAA ATAGAAAATT TTTAAAGAAA
 1081 AAGGAGCCCA TTGGTTTGGA ATAGAATTGC CTACTTGCAT GGACTGCCTC ACAACAGTGA
1141 TTGGACTTAG AAACCTTCAA CCTGTGAAAG AAAAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishimoto, Y., Evolution of BK virus based on complete genome data. J. Mol. Evol., 63, 341-352 (2006). PMID 16897259. [link to RRC of NBRP]