RDB07311 - pGL4-phAQP3

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pGL4-phAQP3 (#RDB07311)

Promoter collection, Human AQP3 promoter

Alternative name	U2663 (2041)-8
Clone info.	PCR amplified human AQP3 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 33448723 to 33447505 (NC_000009.12); relatively -1090 to +129, where +1 corresponds to 1nt of NM_004925.4.
Comment	PCR cloning, forward primer: ggtgaatccccatctccact; reverse primer: gacaccagctccttctgtcg. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human aquaporin 3 (Gill blood group) genomic DNA
Depositor\|Developer	DNA Bank, \| Pan, Jianzhi \|

Remarks, protocol and/or map (pdf)	RDB07311.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07311	pGL4-phAQP3	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phAQP3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07311).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07311_A6Bmp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F () Sequence file: RDB07311_A6Bma.seq
>07311_07311_A6Bm_1_pGL4-4174F_A02_02.ab1 1 ACGAGGGTCA GACATTTCTC TGGCCTAACT GGCCGGTACC GTGTTGCTAG CCTCGAGGAT 61 GGTGAATCCC CATCTCCACT AAAAAAACAA AAATTAGCTG GGCATGGTGG TGTGCACCTG 121 TAATCCCAGC TAATCGGGAG GCTGAAGCAG GAGAATCACT TGAACCTGGG AGGTGGAGGT 181 TGCAGGGAGC CGAGATCACG CCATTGCACT CCACCTAGGC GACAGAGAGA GACTCCGTCT 241 AAAAAAAAAA AAGAGACAGA CTCTTCCTTG GCTGGGGGTA AGTCAGATGG GAGAGGAGAG 301 GGTTAAAAAC AGCTGGGACT CAGCCTGCTG GCAAACATGT GGCATGTGGC ATGTCGGGGC 361 AACTGCAGCT CAGCCTCTGG AGCCATGTGA GCAATGCACG CAGGTACACG TGTGACAAGC 421 TAGGTCACCT AGCCATGTTC AACAGGCATG TGCACAGCCA CGAGGAATGC CCAGCCGTAC 481 AATTAGGCAC ACAGGACATC CGCCATGTGT AGACACAGCT GTGGACATAG CTGGCCAGGA 541 CATGCGACAC ACGACGTGCT CATAGCACAG GGAGAAGGGC CCATGAAGTC TGGTTGGAAC 601 TCAGCACGTG TGTCTGTGTG CCCACCTGAG TCTGGACTGC TGCCCCTCTG ACACTAGCTG 661 TCCCCTTGAA GGGTCGGTGC CTTATCTGTC CTGACAGAAG AGACAGTGTT GCTTCTCACT 721 TGGGGCTCGC AGCCTCCTCC TCCTGCCTCG AACTGAGGAT CTGTTGGGTC CAGTCATCCT 781 GGAGAGATGC GGCCAGTTTC TTTCTGACAG GTCTCCTCCT GCCCGCAAGG AAGTGGGGTG 841 ATCACAGGGC GCAGTGGTCT CTATGACAGC TGCATCCTCT CCAGCCATGG CCCTGAACCC 901 TGCCTATATC CCACCATTGG CTCTCAGATC TGCCTAAGCC TCTCAGCCCC CTTGACGTCC 961 CCCTCCCTTA AGCGCCCTCC GAAGCCACCC GTCCCTCAAA GCTCCTCACA CTTCATGCCC 1021 GCAGCTCCTC CACCCGGCGT CCGCACAAGC TCCAGCGAAG TGGGGCCGGG GGCAAGGGGC 1081 CGCCACTCCT CGGCGCTCGA CTGCAGTCGA CTGCAGCGGC GCCCAGTGTA TCTCCAGCAC 1141 CTACATTA //
Primer: pGL4-136R () Sequence file: RDB07311_A6Bmb.seq
>07311_07311_A6Bm_1_pGL4-136R_B02_05.ab1 1 AGCTTTAGGT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT 61 GGCCGCCGAG GCCAGATCTT GATGACACCA GCTCCTTCTG TCGACCCATG GCGGGGCAGG 121 CGGCGGCGCT GTCGGGCGGG CAGGGGTGGC GGGAGGCGGT GGCGCAGCGA GCAGCGGCCT 181 CCAGCGCTGG TGGCTCCCTT TATAGGAGCG CTGGAGACAC GGGCCCCGCC CGCCCTGCAG 241 CCCCGCCCTG CAGTCCCGGA GCGCCGAGGA GTGCGCGCCC CCTCGCCCCC GCCCCACCTC 301 GGCTGGGAGG CTGGTGCGGA CGCCGGGTGG AGGGAGCTGC GGGCATGGAG TGTGAGGAGC 361 TTTGAGGGAC GGGTGGCCTT CGGAGGGCGC TTAAGGGAGG GGACGTCAAG GGGGCTGAGA 421 GGCTTAGGCA GATCTGAGAG CCAATGGTGG GATTATAGGC AGGGTTCAGG GCCATGGCTG 481 GAGAGGATGC AGCTGTCATA GAGACCACCT GCGCCCTGTG ATCACCCCAC TTCCTTGCGG 541 GCAGGAGGAG ACCTGTCAGA AAGAAACTGG CCGCATCTCT CCAGGATGAC TGGACCCAAC 601 AGATCCTCAG TTCGAGGCAG GAGGAGGAGG CTGCGAGCCC CAAGTGAGAA GCAACACTGT 661 CTCTTCTGTC AGGACAGATA AGGCACCGAC CCTTCAAGGG GACAGCTAGT GTCAGAGGGG 721 CAGCAGTCCA GACTCAGGTG GGCACACAGA CACACGTGCT GAGTTCCAAC CAGACTTCAT 781 GGGCCCTTCT CCCTGTGCTA TGAGCACGTC GTGTGTCGCA TGTCCTGGCC AGCTATGTCC 841 ACAGCTGTGT CTACACATGG CGGATGTCCT GTGTGCCTAA TTGTACGGCT GGGCATTCCT 901 CGTGGCCTGT GCACATGCCT GTTGAACATG GCTAGGTGAC CTAGCTTGTC ACACGTGTAC 961 CTTGCGTGCA TTGCTCACAT GGCCTCCAGA GGCTGAGCTG CAGTTTGCCC CGACATGCCA 1021 CATGCCACAT GTTTTGCCAG CAGCTGAGTC CCAGCTTGTT TTTTACCCTC TTCCTTCTCC 1081 CATCTTGACT TAACCCCCAA GCCAGAGAGT CGTCCTTTTT TTTTTTTAGA CGGGAATCTC 1141 TCCCGATCGC CTTAAGGTGG AATGC //
Primer: pAxCALNL_F1 () Sequence file: RDB07311_A6Bmc.seq
>07311_07311_A6Bm_1_pAxCALNL_F1_C02_08.ab1 1 ATCAATTGTT TTCTTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAAGGAAT 61 TATTACACGG CGATCTTGCC GCCCTTCTTG GCCTTAATGA GAATCTCGCG GATCTTGCGG 121 GCGTCCAACT TGCCGGTCAG TCCTTTAGGC ACCTCGTCCA CGAACACAAC ACCACCGCGC 181 AGCTTCTTGG CGGTTGTAAC CTGGCTGGCC ACATAGTCCA CGATCTCCTT CTCGGTCATG 241 GTTTTACCGT GTTCCAGCAC GACGACTGCG GCGGGCAGCT CGCCGGCATC GTCGTCGGGC 301 AGGCCGGCGA CCCCGGCGTC GAAGATGTTG GGGTGTTGCA GCAGGATGCT CTCCAGTTCG 361 GCTGGGGCTA CCTGGTAGCC CTTGTATTTG ATCAGGCTCT TCAGCCGGTC CACGATGAAG 421 AAGTGCTCGT CCTCGTCCCA GTAGGCGATG TCGCCGCTGT GCAGCCAGCC GTCCTTGTCG 481 ATGAGAGCGT TTGTAGCCTC GGGGTTGTTA ACGTAGCCGC TCATGATCAT GGGGCCACGG 541 ACGCACAGCT CGCCGCGCTG GTTCACACCC AGTGTCTTAC CGGTGTCCAA GTCCACCACC 601 TTAGCCTCGA AGAAGGGCAC CACCTTGCCT ACTGCGCCAG GCTTGTCGTC CCCTTCGGGG 661 GTGATCAGAA TGGCGCTGGT TGTTTCTGTC AGGCCGTAGC CCTGGCGGAT GCCTGGTAAG 721 TGGAAGCGTT TGGCCACGGC CTCACCTACC TCCTTGCTGA GCGGCGCCCC GCCGCTGGGC 781 GATCTCGTGC AAGTTGCTTA GGTCGTACTT GTCGATGAGA GTGCTCTTAG CGAAGAAGCT 841 AAATAGTGTG GGCACCAGCA GGGCCAGATT GAATCTTATA GTCTTGCAAG CTGCGCAGGA 901 ATAAGCTCCT CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC 961 AACGGTGGTT GACATGCCGA AAGCCTTGGT TGAAATGGCA CCCACGCCTG AGAATAGCCG 1021 TTGTCCGGAA TGATTCTGGT TGCCCGAAAA TGGGGGTCGC CCGGCCATGA CTGAAATCCG 1081 ACACCAAGCC CGTGTCGGGT GCGGTAAGCT TAGCCCCTTG GCAATTCGTA CTGCCACTTC 1141 TGTTCCTTGA ATCAGGCCGT ATGGTTTGTT CCCCGGCTCG CTAAGAGCT //