RDB07327 - pGL4-phPLK1

pGL4-phPLK1 (#RDB07327)

Promoter collection, Human PLK1 promoter

Drawn by SnapGene® software

Sequence (full)	RDB07327hts02.seq
Remarks, protocol and/or map (pdf)	RDB07327.pdf
Publication	Unpublished bioresource
Test sheet	RDB20129_B3Kap1-1.pdf

Assembled from experimentally sequenced data.

Alternative name	U2688(2066)-2
Clone info.	PCR amplified human PLK1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 23677730 to 23678950(NC_000016.10); relatively -1042 to +179, where +1 corresponds to 1 nt of NM_005030.6.
Comment	PCR cloning, forward primer: AGGGTTGCCACTCAAGTACC; reverse primer: AGTCACTGCAGCACTCATGC. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Growth conditions	37C, LB+Amp
Gene/insert name	Human polo-like kinase 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07327	pGL4-phPLK1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge