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pGL4-phRB1

Promoter collection, Human RB1 promoter

Catalog number RDB07345
Resource name pGL4-phRB1
Alternative name U2734(2112)-3
Clone info. PCR amplified human RB1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 48302465 to 48303797(NC_000013.11); relatively -1282 to +51, where +1 corresponds to 1 nt of NM_000321.2.
Comment PCR cloning, forward primer: GACGAGTTCAGATGGGCAAT; reverse primer: AACGTCCCCTGAGAAAAACC. Genomic fragment 48,302,464 to 48,303,797 of NC_000013.11 is cloned. Known difference: deletion of 5 nt of 48,302,963 to 48,302,967; T to G substitution at 48,303,180.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human retinoblastoma 1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human retinoblastoma 1

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07345 pGL4-phRB1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07345.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07345_A5Gu.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07345_A5Gua.seq check
          >07345_07345_A5Gu_pGL4-4174F_C04_07.ab1
              1 ACTGGGGCAG GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
             61 ATGACGAGTT CAGATGGGCA ATAACAGTGT CTGCCAAAGA GAAAAAAAAA TGTATTCAAA
            121 CTAGATAATC TATTGGTACA AATACCGAGA CACAGAAGTG ATAACAGCTT TAAGCCAATG
            181 TTTGATGGTG GTAGTCCCAG CAAGCTCTTT TCTGATGTCT TTGTGCCTTT GCACATGCTC
            241 CTTCTCTGTC ACTGTTTTCT TCATCAAACA TAATATAATG GACAAGTGGA ATCAAATAGA
            301 ATTGAGTTCA AATTCTCTGC TACCCATCGG CCCTGGTATT GGACAAATTA ACTCCTCTGA
            361 GCCTGTTTCC TCATCTGCAA CGTAGACTAG CTAATACTAC CCATTGGAAA GCGTTGTTTC
            421 TTAGCTAATG CATGCAAGGC TTAAAACCTA GATGACGGGT TGATAGGTGC AGCAAACCTC
            481 CATGGCATAC GTATGCCTAT GTAACAAACC TACACGTTCT GCACTTGTAT CCCGGAACTT
            541 AAAGTAAAAA AAAAAAAAAA AAAAGAAAGA AAGAAAAAGA AAAAAAAGGC TGTTTCTGGG
            601 GATTAAATAA GACAATTATG TAAGGTGGCC AGCACAGTTC CTGGTACATA GTAAATGTCA
            661 GGCCTGCCTG ACAGACTTCT ATTCAGCAGC TACTGCTCCC CTGAAAATCT TCCTCAGACG
            721 TTTCCACGGT GCTTCCCGTT CTTACACCAC TACAATCCTT TATTACACTA CTCTCCGTTC
            781 ATTCCCCACA GCTCCCTCCC TTCCTTTCCC TAACCAGTGA TCCCAAAAGG CCAGCAAGTG
            841 TCTAACATTT TCTATCTTCT AAGTGACTGG TAAAGTTCCG CACCTATCAG CGCTCCAAGT
            901 TTGTTTTTGT TTTGGCCGAC TTTGCAAAAC GGATTGGGCG GGATGAGAGG TGGGGGGCGC
            961 CGCCCAAAGG AGGGAGAGTG GCGCTCCCGC CGAGGGTGCA CTAGCCAGAT ATTCCCTGCG
           1021 GGGCCCGAGA GTCTTCCCTA TCAGACCCCG GGATAGGATG AGCCCACAGT CACCCCACCA
           1081 GACTCTTGTA TAGCCCCGAT TAAGTGCACC CGACCTTGCA AGGGGTGGCT CTGGCTAGGA
          1141 GGCACCGTCC GGGGCTCG
          //
          Primer: pGL4-136R
          Sequence file: RDB07345_A5Gub.seq check
          >07345_07345_A5Gu_pGL4-136R_D04_10.ab1
              1 GCTATGTTTT GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
             61 CGCCGAGGCC AGATCTTGAT AACGTCCCCT GAGAAAAACC GGACGCGCCC TCCCCCGCCC
            121 GGCAACTGAG CGCCGCGTCC AACCGCGGGA AAACGTCACT TCCGCCCGCG GCGTCACGTC
            181 CGCGAGGCTC CCGGGCCCGC CGGCGTCTGT GGGGAACTGG GGCGCTGGTC GGTGCGCGGG
            241 CTGGGACGCT AAGTCATGAG GAATTAAACT GGGAAACCTG GCGTGGGTCC AGGCGCCTTC
            301 CAGGAGGCAT CCGGCGCGGC CCGGACGTGC TTCTACCCAG AACCACCCCC TCCAGGCCGG
            361 GGTGCACTTA ACGGGGCTAT ACAAAGAGTC TGGTGGGTGA CTGTGGGCCT CATCCCTATC
            421 CCGGGGTCTG ATAGGGAAGA CTCTCGGGCC CCGCAGGGAA TATCTGGCTA GTGCACCCTC
            481 GGCGGGAGCG CCACTCTCCC TCCTTGGGCG GCGCCCCCCA CCTCTCATCC CGCCCAATCC
            541 GTTTTGCAAA GTCGGCCAAA ACAAAAACAA ACTTGGAGCG CTGATAGGTG CGGAACTTTA
            601 CCAGTCACTT AGAAGATAGA AAATGTTAGA CACTTGCTGG CCTTTTGGGA TCACTGGTTA
            661 GGGAAAGGAA GGGAGGGAGC TGTGGGGAAT GAACGGAGAG TAGTGTAATA AAGGATTGTA
            721 GTGGTGTAAG AACGGGAAGC ACCGTGGAAA CGTCTGAGGA AGATTTTCAG GGGAGCAGTA
            781 GCTGCTGAAT AGAAGTCTGT CAGGCAGGCC TGACATTTAC TATGTACCAG GAACTGTGCT
            841 GGCCACCTTA CATAATTGTC TTATTTAATC CCCAGAACAG CCTTTTTTTT CTTTTTCTTT
            901 CTTTCTTTTT TTTTTTTTTT TTTTACTTTA AGTTCCGGGA TACAAGTGCA GAACGTGTAG
            961 GTTTGTTACA TAGGCATACG TATGCCATGG AAGGTTTGCT GCACCTATCA ACCCGTCATC
           1021 TAAGGTTTTA AAGCCCTTGC ATGCATTAGC TAAGAAAACA ACGCTTTCCC AAATTGGGGT
           1081 AGGTATTAGC CTTAGTCTTA CGGTGGCAGA TGAAGCAAAC AGGCTCAGAG GAGTTTAATT
          1141 TGGTCCAAAT ACCCAAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl