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pGL4-phNFATC4 (#RDB07376)

Promoter collection, Human NFATC4 promoter

Alternative name U2725(2103)-5
Clone info. PCR amplified human NFATC4 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 24366849 to 24368163(NC_000014.9); relatively -62 to +1253, where +1 corresponds to 1 nt of NM_001136022.2; relatively -1335 to -20, where +1 corresponds to 1 nt of NM_004554.5.
Comment PCR cloning, forward primer: CCCGTAGAGCCTGTTTCTCA; reverse primer: CCCCCAAACTTCTTCAAAGC. Entire sequence of promoter region has not been confirmed. Known difference (at least): deletion of C at 24368132nt, deletion of 24367993nt, and deletion of CCT at 24367749 to 24367751nt on NC_000014.9.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07376.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07376 pGL4-phNFATC4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phNFATC4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07376).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07376_A1Djp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: pause site, insert 5'
Sequence file: RDB07376_A1Dja.seq check
>07376_07376_B1EcF_pGL4ins_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT
   61 TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGATCCC GTAGAGCCTG
  121 TTTCTCATTA GAGTAACGGG CGCGGTCCCC GCCCGGCTTC TGAGTGTTTG TAAACGTCTG
  181 ACCTGGGGCC GTCGCTTAAC CGTTTAGTTG CTGGGATGGG GCGGCGTTGG GGGTGCGGCC
  241 CTGAACCGGA GGGATTTAGA GACTGGAGAC GCGGCCTCTA AGAGAGGTTG AAACTGTGTG
  301 TGTGTGGGAG AAAATGATAA CCACCCTCCC ATCTCTCCTA CCCGCCAGCC TCGCCAGTAT
  361 CTCCCACCGA GTCACGAATC TCCCATCTAA CTCCCTCTCA CACAACCCAG GCCTCTCCAA
  421 GCCTGACTTT CCCGGAAACT CCAGTCCAGG TCTTCCTTCC TCCTCCAGCC CAGGTGACAG
  481 GTGTCCAGCC TGTTGGGGGC GGGGGGGCCA AGGAGGGGGA AGAGGAGGGG AACCCACAGG
  541 GTCCGGANGT TTCAGGCCCC TAGTAAACCT GGCAAGGANT TTTAGAAGAG CCGGANTGGG
  601 ATGCTATCGG GTCAGANATT CATTCCNGAN TACNGTTCTA GGGANGANGC TGNCNACTTC
  661 CCGNGGANNN TAATTCNNNG NCCNNCTGGG NNGNTGGGNN NNNGNCANCN GNNAANCNNG
721 NNNNNNNNNN
//
Primer: pGL4ins_R (Pr0666)
Region: luc, insert 3'
Sequence file: RDB07376_A1Djb.seq check
>07376_07376_B1EcF_pGL4ins_R_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG CGCTGGGCCC TNNNNNNTGT TTTTGGCATC
   61 TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT TGGCCGCCGA GGCCAGATCT
  121 TGATCCCCCA AACTTCTTCA AAGCGGTCCC CCCCCAGCCT GTCCCTGATT GGCTGCCGGG
  181 GATGCTCCAG CCCCTCCTCC AGGGATGAGA TGGGAAAACC ACGCGCCCCT CGTCCTCCCC
  241 CACCCTCCCT CCCTCTGTCC TCTCTTCAGT CCCTCCCCCT CAAAAAAAAA AAACTGAATT
  301 GGAAAACGGT CCTTCAAGAG TGATACTAAT GTTGCCTATT GGTCCGCCGT TGTGACAATC
  361 ATTTCCCGCA GAGAGCCCAG CCCCCACCTC CCCTAGTCTC AGTTCAAGGT TCTGGGTCCG
  421 AGAGCCTTAA AGGGGCCACA TCCTGGGCCC CCAGGGTAGA GAGCCCACGG AAGACCCTTT
  481 TTTAAATTCT GAAGACTGTG TAGATAGCAC CTAGCAAGGC AGAACCACTT CTCCCCTTTC
  541 CCTCCAAGCC TGAGGCCAGG AGGGTCCTGG GGGCAGAGTG GCACCGAGTT GCTCGGAAGA
  601 NGAAAAGGCT ATTCTTCCGC CTCCCTTGNC NGGGGCTACT CTGCCCGGGC CTTCNGGAAN
  661 TCTTTGCGCT GNCNNANCAG CNNNNCNTCN NCCNTTNNTN NNNNNNCCCN NANGNNGNNN
721 NNNNNNTNNN NNNNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: SV40 polyA, luc
Sequence file: RDB07376_A1Djc.seq check
>07376_07376_B1EcF_pGL4pA_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCNNCA TTTGTAGANN GTTTTACTTG
   61 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG
  121 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
  181 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
  241 TATCTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAGAATTA TTACACGGCG
  301 ATCTTGCCGC CCTTCTTGGC CTTAATGAGA ATCTCGCGGA TCTTGCGGGC GTCCAACTTG
  361 CCGGTCAGTC CTTTAGGCAC CTCGTCCACG AACACAACAC CACCGCGCAG CTTCTTGGCG
  421 GTTGTAACCT GGCTGGCCAC ATAGTCCACG ATCTCCTTCT CGGTCATGGT TTTACCGTGT
  481 TCCAGCACGA CGACTGCGGC GGGCAGCTCG CCGGCATCGT CGNCGGGCAG GCCGGCGACC
541 CCGGCGTCGA ANANGTTGGG GTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles