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pGL4-phINS

Promoter collection, Human INS promoter

Catalog number RDB07387
Resource name pGL4-phINS
Alternative name U2643 (2021)-16
Clone info. PCR amplified human INS promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 2162444 to 2161182(NC_000011.10); relatively -1235 to +28, where +1 corresponds to 1 nt of NM_000207.3.
Comment PCR cloning, forward primer: acctccaggtgcctgttct; reverse primer: cttctgatgcagcctgtcct. Entire sequence of promoter region has not been confirmed. Known difference (at least) : G to C substitution at 2161265nt on NC_000011.10.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human insulin genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human insulin
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07387.pdf

          Distribution information

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          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07387 pGL4-phINS DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phINS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07387).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07387_B4Bkp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F (Pr0665)
          Region: pause site,insert 5'
          Sequence file: RDB07387_B4Bka.seq check
          >07387_07387_B4Bk_3_pGL4ins_F_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNTAGNNN ANNAGNCTGT CCCCAGTGCA AGTGCAGGTG CCAGAACATT
             61 TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC GAGGATACCT CCAGGTGCCT
            121 GTTCTGGGGA GCTGGGAGGG CCGGAGGGGT GTACCCCAGG GGCTCAGCCC AGATGACACT
            181 ATGGGGGTGA TGGTGTCATG GGACCTGGCC AGGAGAGGGG AGATGGGCTC CCAGAAGAGG
            241 AGTGGGGGCT GAGAGGGTGC CTGGGGGGCC AGGACGGAGC TGGGCCAGTG CACAGCTTCC
            301 CACACCTGCC CACCCCCAGA GTCCTGCCGC CACCCCCAGA TCACACGGAA GATGAGGTCC
            361 GAGTGGCCTG CTGAGGACTT GCTGCTTGTC CCCAGGTCCC CAGGTCATGC CCTCCTTCTG
            421 CCACCCTGGG GAGCTGAGGG CCTCAGCTGG GGCTGCTGTC CTAAGGCAGG GTGGGAACTA
            481 GGCAGCCAGC AGGGAGGGGA CCCCTCCCTC ACTCCCACTC TNNNNNNCCC ACCACCTTGG
            541 CCCATCCATG GCGGCATCTT GGNNNNNNCG GGANTGGGGA CAGGGGTCCT GGGGNNNNNN
            601 NGTCCGGGGA CNNGGTCCTG GGGNCAGGGN TGNGAGGANA GGGGTCCCGG GGANNGGGGN
          661 NGNNGGGGNN NNNNGTNCNN GNNNNNNNNG NNTGTNGNNN ANNNGNNNNN NNNNN
          //
          Primer: pGL4ins_R (Pr0666)
          Region: Luc,insert 3'
          Sequence file: RDB07387_B4Bkb.seq check
          >07387_07387_B4Bk_3_pGL4ins_R_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGNANNNNG GCGCTGGGCC CTTCTTAATG TTTTTGGCAT
             61 CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC TTGGCCGCCG AGGCCAGATC
            121 TTGATCTTCT GATGCAGCCT GTCCTGGAGG GCTGAGGGCT GCTGGGCCCC CGCTGGCTTT
            181 ATAGTCTCAG AGCCCATCTC CCCTACCTGT CAACCCCTGC CGCCTGGCCC ATTAGGGCCT
            241 GGGGTGGGGG GGTCGGCAGA TGGCTGGGGG CTGAGGCTGC AATTTCCGGA CCATTTCCCT
            301 GGTGCTGGGT CTGTGGGAAG ATCTCCTTGG TCGTCAGCAC CTCTTCCTCA GGACCAGCGG
            361 GTCATTAGAG TCTTAACCAG GGGCCCGGTG GCCAGACCTG TCCCTGCTCA CAGCTGGGGG
            421 CAAATGTCTC CAGGAGAGCA AAGCCCTCAC CTGGGCCACT TTCCACATTA GACCAGGAGA
            481 GCTGGAGGCT GCAGGGCGGG GCTCTTTGCG CTGCTGTCCC CAGACCCCTG TCCCCAGGAC
            541 CCCTGTCCCC AGGACCCCTG TCCCCAGGAN CCCTGTCCCC ACACCCCTGT CCCCACACCC
            601 CTGTCCCCAC ACCCCTGTCN NNNNACCCCT GTCCCCNGGA NCCCTGTCCC CACACCCCTG
            661 TCCCCAGGAN CCCTGTCCCC ACANNNNTGT CCCCANGANC CCNGNNCCCA CANCNNNNNN
          721 NCCNNNNCNN NNNNNNNN
          //
          Primer: pGL4pA_R (Pr0664)
          Region: SV40 pA,Luc 3'
          Sequence file: RDB07387_B4Bkc.seq check
          >07387_07387_B4Bk_3_pGL4pA_R_D02_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNATCGAN TTNNCCNCAT TTGTAGAGGT TTTACTTGCT
             61 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGNTGTT
            121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
            181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
            241 TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT ACACGGCGAT
            301 CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT CCAACTTGCC
            361 GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT TCTTGGCGGT
            421 TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT TACCGTGTTC
            481 CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC CGGCGACCCC
            541 GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GANGCTCTCC AGTTCGGCTG GGGCTACCTG
            601 GTANCCCTTG TATTTGATNA GGCTCTTCNG CCGGTCCACG ATGAANAANT GCTCGTCCTC
            661 GTCCCANTNN NNGANGTCGC CGCTGNGCAN CCNNCCNTCC TTGNCNANGA NANCGNTTGN
          721 NNNCNNCGGG NNNGNNNNNN NANNCNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2024.03.12

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