RDB07405 - pGL4-phSTAT1

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pGL4-phSTAT1 (#RDB07405)

Promoter collection, Human STAT1 promoter

Alternative name	U2766(2144)-10
Clone info.	PCR amplified human STAT1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 191015482 to 191014170 (NC_000002.12); relatively -1232 to +81, where +1 corresponds to 1 nt of NM_007315.3.
Comment	PCR cloning, forward primer: AGGGGTTTTCCAAACTCAGG; reverse primer: ACTACCCGGCAGGAGAAAAG. Known differences: Compared with NC_000002.12, C to A substitution at 191,015,359nt, insertion of AA between 191,015,243 and 191,015,242nt, T to G substitution at 191,015,207nt, G to A substitution at 191,015,065nt, , G to A substitution at 191,014,834nt and A to G substitution at 191,014,691nt.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human signal transducer and activator of transcription 1, 91kDa genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07405.pdf

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07405	pGL4-phSTAT1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pGL4-phSTAT1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07405).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07405_A1Dip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F (Pr0081) Region: pause site,insert 5' Sequence file: RDB07405_A1Dia.seq
>7405_pGL4-4174F_A04_01_2011-04-19-11-44-21.ab1 1 AGTAGGGTCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG 61 ATAGGGGTTT TCCAAACTCA GGGTGAGAAG TCCCTGAGTT TGGAGAGTGT GGTTTATTTG 121 AAGAACTAAA TGCTGAAGTA AGCAAAGCAG AGTGGCACAT TATGGGGTTG GACAAAGAGA 181 TCGGGAAACC AGGGCTTTAT AGTATGTATT AAGAAACTGA ATTTTATAAG AGCAAAGAAA 241 AGCCATGGAA GGATTTTCAG GTTGGCAAAT GACATGATCT GCTTTTCAAT TAAAAAAAAA 301 AAAACACTCA AACTCTTGTA TGGAGAATGG ATTAAAAGGG AGATAAGGGC TGAAGTGAGG 361 CAGCCATTCG GGAATCTACT GCAGAAGGAG GCAGCAGAAG AGATGGAGCA GAGTAAATGA 421 GACTTGGGAA ATATTTAGGA GGAAGAATCA ATTAAGATTT GAGGATCAGG TGTTGCGAGG 481 TAAGGGAGAA GGCAATGTCA ACTATGAATC CTAGGTATCT GTCATGTGCC ATTTCTAGAA 541 AAAGAGCAGG TTTGAGGAGA AAGATGTTTG GTTCTGAACA TAGTTTGTAG AGCTCACTGC 601 ACATACAAGT GGAGAGGCAA GTGGGAGTTG TAGGTGTGAA GCCCAGAGGA GAGGTGTGGA 661 CGGGATAAGC ATTTAAGACT CCTCCATCTA GAAGGAAACT GAAGCTGTGG GTAAGGTCAT 721 CACAGCACAG CGTTTAGGAG AAGCCCAGGT AAAGAAGCTG ACGAATGTCT GGACCCTGAC 781 AACCTTAACA TATAATGGTT TGATAGTGGA GGTGGAGGCA ATGTAGAAAG AATGCCAGAG 841 GCAGGAAAAA GCAAGGAGGA TGTGTTATCA TCATGACCAA GGAAGAAACG TGTTTCAAGA 901 ACAAAGGCGT CAACTCTGCC CCATGCTTCC GAGCTGTCAA GTAAAGTGAG AAAAACAGAA 961 AAGCGTTCCC TGGGTTTAGC AACACGGAGG TCAGTTGCTA AAGGGAGCTT CTAGAAATGA 1021 CGACGTCGCC CAAATCTGTC TCTGCCTGGA TTCTCGGCGA TGAACCTACC TACAGAGACT 1081 TCCAAAGTTT GGCATCTGCA AACACAGACA CGTCTTTCTG ATCGATTTCT CTTAAGGAAT 1141 ATG //
Primer: pGL4-136R (Pr0079) Region: Luc 5', insert 3' Sequence file: RDB07405_A1Dib.seq
>7405_pGL4-136R_A04_01_2011-04-19-11-44-21.ab1 1 GCTCCTAGGT TTTTGGCATC TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT 61 TGGCCGCCGA GGCCAGATCT TGATACTACC CGGCAGGAGA AAAGGCAGCG CTGGCGCCCG 121 GCCCCCTTCC GCCCCCACCA ATCACCGGGC GGCTCCGCGC TCAGCCAATT AGACGCGGCT 181 GTTCCGTGGG CGCCACCGCC TCCCTCTGCG GGCCGCTGCT CCGCCCACGC GCTGGGGTAT 241 TTCCGCCGGC TTCCGGCGGG GGCCGCCGGC TGGGTGAGCT GCAGCCCAGT CCCGTGTTGC 301 CACGCTGGGA ACTGGCGTTC TGTTTACATA TCTTAGAGAA CGATCAGAAG GACGTGCTGT 361 GTTTGCAGAA GCCCAAACTT GGAGGTCTCT GTAGTAGTTT CATCGCCGAG AATCCAGGCA 421 GAGGACAGAT TTGGCGACGT CGTCATTCTA GAAGCTCCCT TTAGCAACTG ACCTCCGTGT 481 TGCTAAACCC AGGGAACGCT TTTCTGTTTT TCTCACTTTA CTTGACAGCT CGGAAGCATG 541 GGGCAGAGTT GACGCCTTTG TTCTTGAAAC ACGTTTCTTC CTTGGTCATG ATGATAACAC 601 ATCCTCCTTG CTTTTTCCTG CCTCTGGCAT TCTTTCTACA TTGCCTCCAC CTCCACTATC 661 AAACCATTAT ATGTTAAGGT TGTCAGGGTC CAGACATTCG TCAGCTTCTT TACCTGGGCT 721 TCTCCTAAAC GCTGTGCTGT GATGACCTTA CCCACAGCTT CAGTTTCCTT CTAGATGGAG 781 GAGTCTTAAA TGCTTATCCC GTCCACACCT CTCCTCTGGG CTTCACACCT ACAACTCCCA 841 CTTGCCTCTC CACTTGTATG TGCAGTGAGC TCTACAAACT ATGTTCAGAA CCAAACATCT 901 TTCTCCTCAA ACCTGCTCTT TTTCTAGAAA TGGCACATGA CAGATACCTA GGATTCATAG 961 TTGACATTGC CTTCTCCCTT TACCTCGCAA CACCTGATCC TCAAATCTTT AATTTGATTC 1021 TTCCTCCTAA ATATTTCCCA AGTCTCATTT ACTCTGCTCC ATCTCTTCTG CCTGGCTCGT 1081 TCTGCAGTAG ATTCCCGATG GCTGCCTTCA CTCAGCCCGT ATCCTCCCGT TTTAATCCAT 1141 TCCTCTCCAT TACCA //
Primer: SV40pA_R (Pr0882) Region: SV40 pA,Lec 3' Sequence file: RDB07405_A1Dic.seq
>07405_07405_B0L2F_SV40pA_R_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNATTTNNN AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG 61 TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA 121 GAATTATTAC ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT 181 GCGGGCGTCC AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC 241 GCGCAGCTTC TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT 301 CATGGTTTTA CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC 361 GGGCAGGCCG GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG 421 TTCGGCTGGG GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT 481 GAAGAAGTGC TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT 541 GTCGATGAGA GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC 601 ACGGACGCAC AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCNAGTCCAC 661 CACCTTANCC TCNAANAANN GNCACCACCT TGNCTANTGC GCCNGGNTTG NCGTCCCNNT 721 NNGGGGNGAN NNNNNNNGNN NNNNGNNNNN //