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pGL4-phTP53AIP1

Promoter collection, Human TP53AIP1 promoter

Catalog number RDB07410
Resource name pGL4-phTP53AIP1
Alternative name U3302-15
Clone info. PCR amplified human TP53AIP1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 128944146 to 128942842(NC_000011.10); relatively -747 to +558, where +1 corresponds to 1 nt of NM_022112.2.
Comment PCR cloning, forward primer: CCAAAGCTCCACACCTCTTG; reverse primer: ATCTGTGCTGCTGTGACTGG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human tumor protein p53 regulated apoptosis inducing protein 1 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human tumor protein p53 regulated apoptosis inducing protein 1
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07410.pdf

          Distribution information

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          Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07410 pGL4-phTP53AIP1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phTP53AIP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07410).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07410_A3Df.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07410_A3Dfa.seq check
          >07410_07410_A3Df_pGL4-4174F_C01_07.ab1
              1 AGGGGGTCCA GACATTTCTC TGGCCTACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
             61 TCCAAAGCTC CACACCTCTT GCTATCCCAG ACTCTCTGGA ACACCAGGAA GAAAACATAT
            121 TTGGACAAAA ATTATTCTTT GCAACATTAG AACTTTTGAT TCTGGAATGA GACAAGGAAA
            181 TATGTATGTA TCTCAGAGAG ACAATGAATT TTTAAAAGGA AATTGGGACC GTTTCTCAGG
            241 GTGAGATGTC TTATCCGGTT AACTGCGAGC CCCCAGGGAT TTCTACTCAG AGTGTTCATC
            301 ACATCAGGTT TCCATTCTCT ACAATAAAAA CATGGCTAGG GAGAAATTAC CCAGCACTGG
            361 CAAGAAAGAG CTCCAAAATA GAAGCAGCTT TTGAGGAATT TGGAGGGATA AAACGGAATA
            421 GTATGTCTGA AAGTTGGCAA CATGTAAAAA GGCTAAAAAT GACAATTCTG CCTGTGATAC
            481 TATGTCTAGT GTGCAACGCT TCATTCAAAA GGCAGGCACG TCTCCCCTCC AGGCGGGAGT
            541 GGGAATCTCA GTGGGGAACC GGGAACTGCG TTTGAGCTGA TGCAGACTCA GGGTCAACTC
            601 GACAAGGGAG CCCGGCACCC AGACACAGCG GCGTTCACCT CACCTTGCAC CGTCTTTGTC
            661 GGTCTTACAG GCATCAGCTT TCCCAGCCTC ATTTGAGCCG GGATCTGAGT TGCATTTCTT
            721 TCTGGGTCAG GCCTTGCCAT TAGGGAGAGA TGAAGTCAAC TGATGAGCGT GCCAGCCTTG
            781 AGGAAGGACA GAGTGCGGGA AGCAGAGTGT GGGAAGTTGG AAAAAAATAA TCTGCAAGGT
            841 AAACACCTTC AAGTCAATTT CAAGAGGCTG GCTAGAAACC GCCAGGACTT CAGGGGGCCC
            901 TGTCTCGCGG GTCTCCCTGC GCCCTCAGCA CTTTGGCTTG GTATAAGGCC ACCTTGTCCC
            961 TGAAGAGAGG CCTATTTTTA AGCTGAGCAA GAAAAAGGTG AGAAGACTAA AAATCAGCTT
           1021 TTGTGCAGAG ACGCCCAAAT TTTCTGCTGC ACCACATGGT AAACGATGTG CACATTACAG
          1081 CGAGCTTGGC CAAGCTCCCG GACCACGATG GGCGTCCAAA CACTGGT
          //
          Primer: pGL4-136R
          Sequence file: RDB07410_A3Dfb.seq check
          >07410_07410_A3Df_pGL4-136R_D01_10.ab1
              1 GTCTAAGTTT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
             61 GCCGCCGAGG CCAGATCTTG ATATCTGTGC TGCTGTGACT GGGTGCAGGT CTGAGCAGAG
            121 GCTTTCGGAG TGAGCTGAGC AGGCGAGCCC GAGCACGGCC TCTCAGGGAG CGAGCCCATA
            181 GGCTGGGGAG CCGTCCCTGG AGATGCCCCT GGGCTCCTTG CGGGGCATGG CTGAGTTCCT
            241 GCCTGTCCTT GGCAGGCCCG CAGTGGTGGG GTGGCTCAGG CAGTTACCCT TTTCACAACC
            301 AGTGCTGCTG GGGGTGAAGG CAACGGGTGG GTGAGCACGG ACAGTGTGAG CCACGTGGCC
            361 GGGAGCTGGG CCAGCTCGCT GTATGTGCAC ATCGTTTCCA TGTGTGCAGC AGAAATTGGC
            421 GTCTCTGCAC AAAGCTGATT TTAGTCTTTC TCACCTTTTC TTGCTCAGCT TAAAATAGGC
            481 CTCTCTTCAG GGACAAGGTG GCCTTATACC AAGCCAAAGT GCTGAGGGCG CAGGGAGACC
            541 CGCGAGACAG GGCCCCCTGA AGTCCTGGCG GTTTCTAGCC AGCCTCTTGA AATTGACTTG
            601 AAGGTGTTTA CCTTGCAGAT TATTTTTTCC AACTTCCCAC ACTCTGCTTC CCGCACTCTG
            661 TCCTTCCTCA AGGCTGGCAC GCTCATCAGT TGACTTCATC TCTCCCTAAT GGCAAGGCCT
            721 GACCCAGAAA GAAATGCAAC TCAGATCCCG GCTCAAATGA GGCTGGGAAA GCTGATGCCT
            781 GTAAGACCGA CAAAGACGGT GCAAGGTGAG GTGAACGCCG CTGTGTCTGG GTGCCGGGCT
            841 CCCTTGTCGA GTTGACCCTG AGTCTGCATC AGCTCAAACG CAGTTCCCGG TTCCCCACTG
            901 AGATTCCCAC TCCCGCCTGG AGGGGAGACG TGCCTGCCTT TTTGAATGAA GCGTTGCACA
            961 CTAGACATAG TATCACAGGC AGAAATTGTC ATTTTTTAGC CTTTTTTACA TGTTTGCCAA
           1021 ACTTTTCAGA CATACTATTT CCCGTTTTTA TCCCCTCCTA AATTTCCCTC AAAAGCTGCT
          1081 TCTATTTTGC AGGCTCTTTC TGCCAGTGCT GGGGTTAATT TTTCTT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Igarashi, H., Anti-apoptotic roles for the mutant p53R248Q through suppression of p53-regulated apoptosis-inducing protein 1 in the RA-derived fibroblast-like synoviocyte cell line MH7A. Clin. Immunol. 150 (1): 12-21 (2014). PMID 24316591.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl