RDB07413 - pGL4-phSESN2

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pGL4-phSESN2 (#RDB07413)

Promoter collection, Human SESN2 promoter

Alternative name	U3307-10
Clone info.	PCR amplified human SESN2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 28258366 to 28259686(NC_000001.11); relatively -1152 to +169, where +1 corresponds to 1 nt of NM_031459.5.
Comment	PCR cloning, forward primer: AAGTGCCTTGGGAGCTCAG; reverse primer: CGCTTTGGTGCTGGACTCT. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human sestrin 2 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07413.pdf

Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07413	pGL4-phSESN2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phSESN2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07413).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07413_A1Aqp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665) Region: pause site, insert 5' Sequence file: RDB07413_A1Aqa.seq
>07413_07413_B1K5F_pGL4ins_F_A09_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNANN AGGCTGTCCC CAGTGCAAGT GCAGGTGCCA 61 GAACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG GATAAGTGCC 121 TTGGGAGCTC AGGGGAGGGT GAGATTAATC TCTTTTTCCC AAGAATCTGG AGGCAGGAAA 181 CCAAGTCGGT TTCATCTTTG GAATTGTAAG CTTAGACTTA GAAGTCTCAG AGGGGGACTT 241 GGGGGTCTTA TCCACCCTCA CCCCTGGCAG GATTCCCTGT CTAGAATAAA CAATAACTTT 301 AAAATGGAAA CAAAAAAAGG AGCTCTGAAG GAAAACTGTG AAGGAAGAGG CTCCGTTTGA 361 AATGAATAGA AAATGAATTT AGCTTGGAGG TGCTGAGGTG TTGGTAGAGG CAACTTTCTG 421 GCAAGAGGCG AGTTGAAGGC CAGGAAGTGA ATGAGATCAG GGAGAGTGTC TGGAGGGAAG 481 AGGGGCAAAA ACAAACTGGG GAAGCACCCA CGTTTTAGGA AGTGGGTGGT GAAAGAGGAG 541 TCAGAGAAGG CCAGAGGGAG AGGTGAGCAA AGATCCAGCA GAACACCTTG TTACAAAGTT 601 TCGAGGAGGG TGGGAGGGGT GGAAGCCAGG ACGGGTAGCA GAAGGAAGAA AACTTCCAGC 661 AATAGGGATT GAGGTTCCAC ACGAAGTTAC CAATGACATT TCAAGNNAAT GAAGGCTTTT 721 CCTGANGCTC CACAAATAAN GTGAATTCCC TGTGTCTCGC ATCTTTGGAG CTTCCCCGAG 781 GCCAGGGGGA GCAGGGTTGT GCCNTCTGAN CTCTAAATAT CTGGGGGTNC AGTTCTATAA 841 TTAGCANCAC CGGATANGAC GCGAGCCAAN NNTCNCTTAN CCCTANCCAG TCCCTCNNGG 901 AACTGAAAAN GATGANNNAN TTCATTTAGG AGANTTNCAC ANNCNGCNNT TTNGGNCTNN 961 NNNNNNGNAN NNNCNNTTTN NATCANNNNN NCCCNAANGN NNNNNNNNN //
Primer: pGL4ins_R (Pr0666) Region: luc 5',insert 3' Sequence file: RDB07413_A1Aqb.seq
>07413_07413_B1K5F_pGL4ins_R_B09_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNNNNGNGCT GGGCCCTTCT TANTGTTTTT 61 GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC CGCCGAGGCC 121 AGATCTTGAT CGCTTTGGTG CTGGACTCTT CCCCCAGTCG GGCTTCGGGA ACGGCGAACG 181 GCCTCGGGGG CCTGGGAAGG GCCGCAGCGC AGGGGTTTTC ACGGCCTCGG AAGTCCGCAG 241 GGGCCAGGGC TGCTCCCGGA CCCAGCGCTC CTCAGCATCC CCGCCGCGGA ACTGCCAGCG 301 CTCGCCGGGC TCTGACACCA GCAGTTCAGC CCGCTGCGGT CCCCGGGGGA GGGCGGGGAC 361 ACAAAAGCCC GGCGGTCCAG CCAATCAGAG GTCTGTTTCC CCGGGGAGGC GGGGGAAACT 421 CCGCCCCCTG GAGTCACGGG CTCCGCCAAT CAGCACCGGG GAGCGCCCGG AATTCTGGGA 481 GTTGTAGTCC GCCTCGGGTG AATGCTGCAA ACTTTTGCAA GACGGTTTAG TCAGATCCCT 541 GGCAACACCA GACGGCAGTG ACAGCCCTAA GAGTCATTAG GGTTGCGTGA TGAAAAGGGA 601 CTTCACTGGG CTAGGCNNNN NNGCCGAGTG TGAAATCTCC TAAATGAATT CTTCATCTTT 661 TCAGTTCCTG GAGGGACTGG CTAGGGTTAA GAGAGGTTTG GCTCGCGTCC TATCCGGTGC 721 TGCTAATTAT AGAACTGAAC CCCANATATT TAGAGGTCAG ANGGCACAAC CCTGCTCCCC 781 CTGGCCTCGG GGAAGCTCCA AAGATGCGAG ACNCAGGGAA TTCACCTTAT TTGTGGANCC 841 TCAGGAAAAG CCTTNCATTT NCTTGAANNG TCNNTNGNAA CTNNGNGTGN NANNNTCNAT 901 CCCTATNGCT GGAAGTTTTC TTCCTNNTGC TACCCGTNCN NNGCNNNNNN NCCCTCCCNN 961 CCNTNNNNGN ANNNNTTNNN NANNNNNGNN GNTTCNNNNN NGNANCTTTN GNNNCNNNTN 1021 NNCNNNNNNN NCNNNNNNNN NN //
Primer: SV40pA_R (Pr0882) Region: SV40 pA,luc 3' Sequence file: RDB07413_A1Aqc.seq
>07413_07413_B1K5F_SV40pA_R_C09_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNAAAGCAT TTTTTNNNNN GCATTCTANN 61 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC 121 CCCGACTCTA GAATTATTAC ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT 181 CGCGGATCTT GCGGGCGTCC AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA 241 CAACACCACC GCGCAGCTTC TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT 301 CCTTCTCGGT CATGGTTTTA CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG 361 CATCGTCGTC GGGCAGGCCG GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA 421 TGCTCTCCAG TTCGGCTGGG GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC 481 GGTCCACGAT GAAGAAGTGC TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC 541 AGCCGTCCTT GTCGATGAGA GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA 601 TCATGGGGCC ACGGACGCAC AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT 661 CCAAGTCCAC CACCTTAGCC TCGAAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG 721 TCGTCCCCTT CNGGGGTGAT CAGAATGGCG CTGGTTGNTT CTGTCAGGCC GTANCCCTGG 781 CGGATGCCTG GNANGTGGAA GCGTTTGGNC NCNGCCTCAC CTACCTCCTT GCTGAGCGGC 841 NNCCCGCCNG CTGGCGATCT CGTGCAAGTT GCTTANGTCG TACTTGNCNA NNNANAGTGC 901 TCNTANNGAA NNANCTAANN NNTGNGGGNC NNCNNCAGGG CAGANTGANT CTTATNNTCT 961 TGCAAGCTGC GCNNNNAANN NNCTNCNNNN NNNNNANNNG NTNNNNNNAN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report	Park, S.J., Protective effect of sestrin2 against iron overload and ferroptosis-induced liver injury. Toxicol. Appl. Pharmacol. 379: 114665 (2019). PMID 31323261. [link to RRC of NBRP]
user_report	Jegal, K.H., Activating transcription factor 6-dependent sestrin 2 induction ameliorates ER stress-mediated liver injury. Biochim. Biophys. Acta 1864 (7): 1295-1307 (2017). PMID 28433684. [link to RRC of NBRP]
user_report	Reddy, K., Dysregulation of Nutrient Sensing and CLEARance in Presenilin Deficiency. Cell Rep. 14 (9): 2166-2179 (2016). PMID 26923592. [link to RRC of NBRP]
user_report	Jegal, K.H., Eupatilin induces Sestrin2-dependent autophagy to prevent oxidative stress. Apoptosis 21 (5): 642-656 (2016). PMID 27015669. [link to RRC of NBRP]
user_report	Jin, S.H., Resveratrol inhibits LXR alpha-dependent hepatic lipogenesis through novel antioxidant Sestrin2 gene induction. Toxicol. Appl. Pharmacol., 271 (1): 95-105 (2013). PMID 23651738. [link to RRC of NBRP]
user_report	Yang, J.H., Isorhamnetin protects against oxidative stress by activating Nrf2 and inducing the expression of its target genes. Toxicol. Appl. Pharmacol., 274 (2): 293-301 (2013). PMID 24211276. [link to RRC of NBRP]
user_report	Shin, B.Y., Nrf2-ARE pathway regulates induction of Sestrin-2 expression. Free Radic. Biol. Med., 53 (4): 834-841 (2012). PMID 22749810. [link to RRC of NBRP]