RDB07485 - pGL4-phHMOX1

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pGL4-phHMOX1 (#RDB07485)

Promoter collection, Human HMOX1 promoter

Alternative name	U2826-6
Clone info.	PCR amplified human HMOX1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 35,379,656 to 35,381,125 (NC_000022.11); relatively -1,440 to +30, where +1 corresponds to 1 nt of NM_002133.3.
Comment	PCR cloning, forward primer: gctgtagtggccctaggctgaa; reverse primer: GCTGAGGACGCTCGAGAGGA. Entire sequence of promoter region has not been confirmed. Known differences (at least): T to G at 35,379,705nt, G to T at 35,379,706nt, and A to G at 35,379,896nt of NC_000022.11.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human heme oxygenase (decycling) 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07485.pdf

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07485	pGL4-phHMOX1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phHMOX1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07485).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07485_A9Gpp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F () Region: insert5' Sequence file: RDB07485_A9Gpa.seq
>07485_07485_A9Gp_pGL4ins_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNAAA CNNNNNNNNN NNNNGCTGTC CCCAGNGCAA GTGCAGGTGC 61 CAGAACATTT CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG AGGATGCTGT 121 AGTGGCCCTA GGCTGAATTC CAGGAAGTTT TTTTTTTTTT TTTTGTTTTT TTTTTTTTTT 181 NNNNNNNNNN //
Primer: Luc_R () Region: insert3' Sequence file: RDB07485_A9Gpb.seq
>07485_07485_A9Gp_Luc_R_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNANCGCTT NNTGGCTTTG TGCAGCTGCT CGCCGGCGGT 61 CCCGTCTTCG AGNNNNNNNN NNGNNGCTGG GCCCTTCTTA ATGTTTTTGG CATCTTCCAT 121 GGNGGCTTTA CCAACAGTAC CGGATTGCCA AGCTTGGCCG CCGAGGCCAG ATCTTGATGC 181 TGAGGACGCT CGAGAGGAGG CAGGCGTTGA CTGCCGGAGC CGCGGCCGGT CACATTTATG 241 CTCGGCGGGT CACGTGGGCC AGGCGGAACA GCTGATGCCC ACTTTCTGGC CGGGCGTTGC 301 AACACCCGCA GGGGCCCGCG CCCAGCCCGC CCCGGGCCAG TGTGGGGTGG AGAGGAGCAG 361 TCATATGACC CTTGGGAAAC AAAGTCTGGC CATAGGACTT TTAGAGAAAA CACACACACA 421 CACACACACA CACACACATA CACACACACA CACACACACA CACACACACA AAACCTTTAA 481 GGAAATCTGA GAAGCTGCAG GCTCTGGGTG TGATTTTGCT CCTTCCAGAG CCCCCCCTTC 541 TGCTCCCTGT CCCCTTGGGA CTTGATGCAC CCCAGGCGTC CCAGAAGGTT CCAGAAAGCT 601 GGGAGGCAGC GCTGCTCAGA GCAATAGCCT GGTGGGCAAC ATCAGGAACT TAGCAACCCC 661 AGGCTCTGCT GTCTCCAGCT CCCTAAAATG TCACCTGCTG GAATCCTCTG CTGACTGCTT 721 GCCTCCTCAC ACCCTCTTTA AATGTACATT TAGGATCTGT TTTTTGAGTT CCTGTCAAAA 781 AACAGAGACT CTAGTATTTT CATGGTTCAT CCCTACAACT GACCTGTGAG GGTAGATCGC 841 TTCTATTTTA CAGATGGGGA AACNGANGCT CANAGGGGTG CAGNGNNGTC ACACATAAAG 901 CANGATGCCN GNGTNNNAAN CNNGGGNNGA NNNTAANNNC CCGNNNGNNN NCTNCNCACT 961 NNGGNNANCA CNNNNNNTNN NNNNNNNN //
Primer: hHMOX_R2 () Region: insert Sequence file: RDB07485_A9Gpc.seq
>07485_07485_A9Gp_hHMOX_R2_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNT NNNNNCTGNN TTNTACTCNG TGCCTGCTCN GGGCCCAGAG 61 CAGAGCTGGC CCTTGCTGAT CGCCTATTGA ATCAATTCTG CATGAGGTGG ACTCCTTAGA 121 ACTCGGGGGA AATAGGGTCA CTGGGTGAAC CCAGAGGACA AAACGAATGG ACCAGATGAT 181 TCATACAGTC CTTTCCCAAT ACTGTGATTC TCATTCTAAG ATTTTTTTTT TAAGATGGCT 241 TTCAGGCCTG GCATGGTAGC TCACACCTGT AGTCCCAGTA CTTTGGGAGG TTGAGGTGGG 301 AAGAGCGCTT TGAGCCCAGT CGAGGTGGGA AGATTGCTTT GAGTTCGAGA CTGGCCTGGG 361 CAACATAGGG AGACCCCGTC TCTACAAAAT ATAAAATAAA TTAGCTGGGC ATGGTGGCAC 421 ATGCCTGTGG TCCCAGCTAC TTGGGAGGCT GAGGCTGGAG GTTCACTTGA GCCTGGGATG 481 TAGAGGCTGG AGTGAGCTGT GACCACGCTA CTGTATTCTA ACCTGGGCAA CAGAACAAGA 541 CGCTGTCCCT CAAAAAAAAA AAAAAAACAA AAAAAAAAAA AAAAACTTCC TGGAATTCAG 601 CCTAGGGCCA NTACAGCATC CTCGAGGCTA GCGAGCTCAG GTACCGGCCA GTTAGGCCAN 661 AGAAATGTTC TGGCACCTGC ACTTGCACTG GGGACAGCCT ATTTTGCTAG TTTGTTTTGT 721 TTCGTTTTGT TTTGATGGAG AGCGTATGTT AGTACTATCG ATTCNCACAA AAAACCAACA 781 CACAGATGTA ATGAAAATAA AGATATTTTA TTGCGGCCTG TCCAATACCT CCCGTACCTT 841 AATATTACTT ACTTATCCTT GANAGANNNA CNANNAACCN NGANAAATGC TTCNNTNATA 901 TTGAAAANNG NNNNANTATG ANCNTCCNNC NTTTTCGNGN NNNNNNCNTT NCNNNNCTTT 961 GCGGNNTTTN GCTNNNNNNN TTTTNNNNNC CCCNNAAACN NNNNNNNNAN NNNAAANNNN 1021 NNNNNN //
Primer: pGL4pA_R () Region: SV40 p(A),Luc. Sequence file: RDB07485_A9Gpd.seq
>07485_07485_A9Gp_pGL4pA_R_D10_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNT TATCGNNTTN NCNNNNTTTG TAGAGGTTTT ACTTGCTTTA 61 AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT 121 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA 181 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT 241 TATCATGTCT GCTCGAAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA CGGCGATCTT 301 GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA ACTTGCCGGT 361 CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT TGGCGGTTGT 421 AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC CGTGTTCCAG 481 CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG CGACCCCGGC 541 GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG CTACCTGGTA 601 GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT CGTCCTCGTC 661 CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG CGTTTGTAGC 721 CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA GCTCGCCGCG 781 CTGGTTCACA CCCAGTGTCT TANCGGTGTC CAAGTCCACC ACCTTANCCT CGAANAANGG 841 CACCACCTNN NNTACNGCGC CAGGCTNGNC GNCCCNNTCN GGGGNGATCA GAATGGCGCT 901 GGTTGTTTCT GTCANGNCGN ANCCCNNGNN NNNGNCNGGT NNNNNNNANG CGNTTGGNNN 961 CGGCNNCNNN TANNNNCNTT GCNNANCGGN NNNNNNNNN //