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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-Venus-NLS (#RDB07563)

Lentivirus vector plasmid of Venus fluorescent protein with NLS driven by human EF-1 alpha promoter.

Clone info. Lentivirus vector plasmid of Venus fluorescent protein with NLS driven by human EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-MCS (RDB04378) (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB07563z.seq
Remarks, protocol and/or map (pdf) RDB07563.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07563 CSII-EF-Venus-NLS DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-Venus-NLS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07563).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18579_B0I9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1alpha pro,Venus 5'
Sequence file: RDB18579_B0I9a.seq check
>07563_18579_B0I9_2_EF1a-C_F_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNTTTTN AGTTTGGNNC TTGGTTNNTT CTCNAGCCTC AGACAGTGGT
   61 TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC GTGAACACGC TACCGGTCTC GAGAATTCTG
  121 CAGCGGCCGC GGATCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
  181 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG
  241 CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA CCGGCAAGCT
  301 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG
  361 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT
  421 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA
  481 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA
  541 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAC
  601 CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA
  661 CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
  721 GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
  781 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCNT
  841 GGANGANCTG TACAAGTCCG GANTCANATC TCGANCTGAT CCAAAAAAGA AGANAAAGGT
  901 AGATCCNAAA AAGAAGANAA NGGTANATNC AAAAAANAAN ANAANGGNNN NATCCTCTAG
  961 CNANAGTTNN NNTCGAGGGA TCNNGCTNAT CNATNNTNNN NCNTCNNGNA NNANNANANT
1021 NNNNNANNNN NNNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE,3x SV40 NLS,Venus 3'
Sequence file: RDB18579_B0I9b.seq check
>07563_18579_B0I9_2_PRE_rev_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNN AGCNNCNTAG TTAAGAATAC CAGTCAATCT TTCACAAATT
   61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGCTAGAG
  121 GATCCTACCT TTCTCTTCTT TTTTGGATCT ACCTTTCTCT TCTTTTTTGG ATCTACCTTT
  181 CTCTTCTTTT TTGGATCAGC TCGAGATCTG AGTCCGGACT TGTACAGCTC GTCCATGCCG
  241 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
  301 GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG
  361 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCTCG
  421 ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA
  481 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC
  541 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG NTCACCAGGG TGTCGCCCTC NAACTTCNCC
  601 TCNGCGCGGG TCTNGNANTN GCCGNCGNCC TTGNANAANA NNGNGNGCTN NNNNNNGTAN
  661 NCNTNCNGGN NTNNNNNANN NGNANANNTC NNGNTGNNTN NNGNNGNNNN GGNNANNNGN
721 NNNANNNNTG NNNNNNNANN NNNNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,5' LTR, HIV-1 psi
Sequence file: RDB18579_B0I9c.seq check
>07563_18579_B0I9_2_CMV_Forward_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNC NNNTGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC
  121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA
  181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA
  241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA
  301 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA
  361 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG
  421 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA
  481 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA
  541 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA
  601 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT
  661 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA
  721 AGGAAGCTTT AGACNAGATA GAGGAAGAGC AAAACNAAAA GTAAGACCAC CGCACAGCAA
  781 GGGCCGCTGA TCTTCAGACC TGGANGAGGA GANATGAGGG ACAATTGGAG AAGTGAATTA
  841 TATNAATATA ANGNANNNAA AATNGAACCA TTANGANTAN NACCCANNNA GGNNAAGANA
  901 ANANNGGNGC AGANANNAAA AANANCNNNT GGGAATNGGA NCTTTGNTCC TTGGGTTCTT
  961 GGGAGCANCN NNNNCACTNN GGGGCGCNNC NNNNNNGNNN NNCNNNNNGN NCANGNCCNN
 1021 ANAATTNNTN NNNNNGGNNA NNNNNGNNAN CANNNNNANN NNNNTTNNNN NNNNGGNNNN
1081 NNNNNN
//
Primer: cPPT/CTS-E_R (Pr0725)
Region: cPPT_CTS,RRE
Sequence file: RDB18579_B0I9d.seq check
>07563_18579_B0I9_2_cPPT+CTS-E_R_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA GTTTGNATGT CTGTTGCTAT TATGTCTACT
   61 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT
  121 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC
  181 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA
  241 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT
  301 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT
  361 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC
  421 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT
  481 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCAGAGATTT
  541 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAA ATGAGTTTTC CAGAGCAACC
  601 CCAAATCCCC AGGAGCTGTT GATCCTTTAG GTATCTTTCC ACAGCCAGGA TTCTTGCCTG
  661 GAGCTGCTTG ATGCCCCAGA CTGTGAGTTG CAACAGATGC TGTTGCGCCT CAATAGCCCT
  721 CAGCAAATTG TTCTGCTGCT GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG
  781 CGTCATTGAC GCTGCGCCCA TAGTGCTTCC TGCTGCTCCC AAGAACCNNA GGAACAAAGC
  841 TCCTATTCCC ACTGCTCTTT TTTCTCTCTG CNCCACTCTT CTCTTTGCCN TGGTGGGNGC
  901 TACTCCTAAN GGTTCNATTT TTACNACTTT ATATTTATAT AATTCACTTC NNNNATTGTC
961 CCNCATATCT NCNNCNCCAN GNCTGANNAT CANCNGNNCC TTGCTGNGNN NNNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGHpA,3' LTR, PRE
Sequence file: RDB18579_B0I9e.seq check
>07563_18579_B0I9_2_bGH_rev3_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNNNN NGGNNGGGCN NACNANNGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
  121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
  181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
  241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
  301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
  361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
  421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
  481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
  541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
  601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
  661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
  721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
  781 GACGGTATCG ATGCGGGGAG GCGGNCCAAA GGGAGATCCG ACTCGTCTGA NGGCGAANGC
  841 GAAGANGCGG AAGANGNCGC ANANCCGGCA GCANGCCGCN GGAAGGAANG TCCGCTGGAT
  901 TGANGGCCNA ANGGANNTAN NANAANNNAN GTCCCGCGCA NAATCCNNGN GGNNNNNCAG
961 GNNANCANNN NTNNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,Zeo,SV40pA
Sequence file: RDB18579_B0I9f.seq check
>07563_18579_B0I9_2_SV40pro_ori_F_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCTCTGCCT CTGAGCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
  181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
  241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
  301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
  361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
  421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
  481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
  541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
  601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
  661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
  721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  781 ACTGCATTCT AGTTGTGGTT TGTCCNAACT CATCAATGTA TCTTATCATG TCTGTATACC
  841 GTCNANCTCT ANCTAGAGCT TGNCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG
  901 TTATCCGCTC NCAATTNCNC ACAACATACN AGCCNGANNC ATAAAGTGTA AAGCCNGGGG
  961 NGCNTNNTGA GNGANCTANC TCNNNTTANN NNCNNNGCGC NNNCNGNCNN NNTNNCNNNN
1021 NGGNNAANCN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles