Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1(+)cs-h Integrin alpha5

Expression vector of human integrin alpha5.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

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Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]

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提供条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07658_A9Kbp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,attB1,I-CeuI,insert5' Sequence file: RDB07658_A9Kba.seq
>07658_07658_A9Kb_2_CMV-Forward_B11_05_ABI24.ab1     1 NNNNNNNNNN NNNNNTGNNG CNNNGGGCGG TANGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC   121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGGTAC CGAGCTCGGA   181 TCCACAAGTT TGTACAAAAA AGCAGGCTTG TAAAACGACG GCCAGTAACT ATAACGGTCC   241 TAAGGTAGCG AGGCCTGGGT GGCGAATTCG GCACGAGGGT TTAGGAAGCG GCTCCGGGTC   301 GGTGGCCCCA GGACAGGGAA GAGCGGGCGC TATGGGGAGC CGGACGCCAG AGTCCCCTCT   361 CCACGCCGTG CAGCTGCGCT GGGGCCCCCG GCGCCGACCC CCGCTGCTGC CGCTGCTGTT   421 GCTGCTGCTG CCGCCGCCAC CCAGGGTCGG GGGCTTCAAC TTAGACGCGG AGGCCCCAGC   481 AGTACTCTCG GGGCCCCCGG GCTCCTTCTT CGGATTCTCA GTGGAGTTTT ACCGGCCGGG   541 AACAGACGGG GTCAGTGTGC TGGTGGGAGC ACCCAAGGCT AATACCAGCC AGCCAGGAGT   601 GCTGCAGGGT GGTGCTGTCT ACCTCTGTCC TTGGGGTGCC AGCCCCACAC AGTGCACCCC   661 CATTGAATTT GACAGCAAAG GCTCTCGGCT CCTGGAGTCC TCACTGTCCA GCTCAGAGGG   721 ANAGGAGCCT GTGGAGTACA AGTCCTTGCA GTGGTTCGGG GCAACAGTTC GAGCCCATGG   781 CTCCTCCATC TTGGCATGCG CTCCACTGTA CAGCTGGCGC ACANNNAANG ANCCACTGNN   841 NGACCCCGNG GGNCACCNGC TACNTCTNCA CANNTAANTT CACCCNAATT CTGNNNTATG   901 CACCCNGCNG CNCANNNTTC ANCNNGNNNN CNNNACAGGN NNNNNGNCNA NNNNNGNNNN 961 NNNNNN //
Primer: bGH-M_R (Pr0747) Region: bGHp(A),attB2,PI-SceI,insert3' Sequence file: RDB07658_A9Kbb.seq
>07658_07658_A9Kb_2_bGH-M_R_C11_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN GGGCNNNCAA CAGNTGGCTG GCAACTAGAA GGCACAGTCG    61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCCAC TGTGCTGGAT   121 GGGTACTGCA GCCGATTCAT TAATGCAGGT TAACCTGGCT TATCGAAATT AATACGACTC   181 ACTATAGGGA GACCGGCAGA TCTACCACTT TGTACAAGAA AGCTGGGTCA GGAAACAGCT   241 ATGACCATGT GCCATTTCAT TACCTCTTTC TCCGCACCCG ACATAGATGC CTCGAGTTTT   301 TTTTTTTTTT TTTTGGTCAG TGGGGGCACT GGGTCANATN NATTGCACTT GCAACANANT   361 TTAAATAANT CCTGGGTGTC TGGTGCCAAG GTGAGGGAAG GGTTGGGCAN ANANATGAGG   421 GGCAGCATCA NTGCAGCTGG CAGGCANAAC CCAAATTCTG CAGGCCCAGG ACAGTGGGCT   481 CCCCTTTCTC TGGGGAACAG GGAGGGCCTG TGTCTGGCCA GGCTGANGTT CCANATNNGT   541 TGCCATCATG GCCCCTTCNN GGTCCTGGGA AATTCCTGGC TTCTCCTAAA TCANGGTGAA   601 CTGGGCCTCC AGNATCANGT CTGGAGCANG CCCAAATATA GTCCTGGATC TGCCTGGATT   661 ANGTGCCAAT GTCTGANTCT GGGTTCCANA TNNACTCCNG ACNCCAGGCT GGATCTGNNC   721 CCATTTGAGT TCTGATTCCC CTNGGANCTG GGCTCTGGGC CCTGGGCCAG CATCTATCCN   781 TGTGTGGNAT CTGTCCTGAN CTGGTCCTGG GGCANCNTGC ATAGTTAGTG NTCTTTGTTG   841 GCCCNCCAGG GNGGGGNTGT NNNGANCTGN NNNGNNNTNN NNNNNNNAGT TNCNNNNNNN 901 TANNNNGGGG NNNNNTTTGG NTNTC //
Primer: hITGA5-B_F1 (Pr0777) Region: insert3' Sequence file: RDB07658_A9Kbc.seq
>07658_07658_A9Kb_2_hITGA5-B_F1_A05_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NAGNNGCTGG CCCNGGGCCC NGNNCCCAGC TCCNAGGGGA    61 ANCAGAACTC AAATGGGGCC AGATCCAGCC TGGGGTCTGG AGTTGATCTG GAACCCAGAC   121 TCAGACATTG GCACCTAATC CAGGCAGATC CAGGACTATA TTTGGGCCTG CTCCAGACCT   181 GATCCTGGAG GCCCAGTTCA CCCTGATTTA GGAGAAGCCA GGAATTTCCC AGGACCCTGA   241 AGGGGCCATG ATGGCAACAG ATCTGGAACC TCAGCCTGGC CAGACACAGG CCCTCCCTGT   301 TCCCCAGAGA AAGGGGAGCC CACTGTCCTG GGCCTGCAGA ATTTGGGTTC TGCCTGCCAG   361 CTGCACTGAT GCTGCCCCTC ATCTCTCTGC CCAACCCTTC CCTCACCTTG GCACCAGACA   421 CCCAGGACTT ATTTAAACTC TGTTGCAAGT GCAATAAATC TGACCCAGTG CCCCCACTGA   481 CCAAAAAAAA AAAAAAAAAN NTCNAGGCAT CTATGTCGGG TGCGGAGAAA GAGGTAATGA   541 AATGGCACAT GGTCATAGCT GTTTCCTGAC CCAGCTTTCT TGTACAAAGT GGTAGATCTG   601 CCGGTCTCCC TATAGTGAGT CGTATTAATT TCGATAAGCC AGGTTAACCT GCATTAATGA   661 ATCGGCTGCA GTACCCATCC AGCACAGTGG CGGCCGCTCG AGTCTANAGG GCCCGTTTAA   721 ACNCGCTGAT CAGCCTCNAC NGTGCCTTCN ANTTGCCAGC CNTCTGTTGT TTGCCCCTCC   781 CCCGNGCCTT CCTTGACCCN NNNANGGNGN CNCTCCCACT NNNCTTTNNN NNNNAAANNA   841 NNAAANTGCN NCNNNNNNNC NNNNTNNNNN NCNNTNNNNN CTGGGGGNNN NGGGNNNNGG 901 GNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB07658_A9Kbd.seq
>07658_07658_A9Kb_2_SV40pro_ori_F_D11_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CTGCCTCTGN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT    61 TGGAGGCCTA NGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA   121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC   181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG   241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC   301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC   361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG   421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA   481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG   541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG   601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT   661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGATCTC GTCGTGACCC ATGGCGATGC   721 CTGCTTGCCG AATATCATGG TGGANNATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG   781 GCTGGGTGTG GCGGANCGCT ATCAGGACAT AGCGTTGGCT ACCCGNGATA TTGCTGAAGA   841 NCTTGGCGGC GAANGGGCTG NNCGCTTCCT CGNGCTTTAC NGNATCGCCG CNNCNNANNC   901 GCANCGCATC GCCNTCNANN GCNNNNNNNN CNANNNCTTN NNANCGGGNN NNNNGGGNNN 961 NNAANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl