RDB07691 - pGL4-phBRCA2

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pGL4-phBRCA2 (#RDB07691)

Promoter collection, Human BRCA2 promoter

Alternative name	U3017-2
Clone info.	PCR amplified human BRCA2 promoter sequence was inserted into a firefly luciferase vector. Genomic DNA (32,314,131 - 32,315,604nt of NC_000013.11) corresponding to 1 to 125nt of NM_000059.3 and 1.3 kb up-stream was cloned.
Comment	PCR cloning, forward primer: cccaaagcagaatgcctaac; reverse primer: atctgtcccctcacgcttct. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human breast cancer 2, early onset genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07691.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07691	pGL4-phBRCA2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phBRCA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07691).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07691_A7Jjp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F () Region: pause site,insert5' Sequence file: RDB07691_A7Jja.seq
>07691_07691_A7Jj_pGL4-4174F_D01_04_ABI08.ab1 1 AGGGGTCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT 61 CCCAAAGCAG AATGCCTAAC TCATAAAATG TTTGGTAAGT GGCAGCTGTT ATTAGTAAGG 121 ATCACAGACG TATTTCTTTG CGAATTAGAC TTAGAGCAAA AGCTTTAGAA TCTATCTGCA 181 TTAGTAAGGC CTCCTTCTAC AACTAAGGAT TACTGATTAT CAAACTAAAA TGGTCCCTAA 241 GGAACCAAGC GGAGCCCACC CTTTTCCTAA TCCAAGGTTG CTTCTGTTTA CTGCTCAAGC 301 ACCTTCTGGA AGCAGCAAGG CCCCCATGGG AGCAACTCTC ACTGAATCCA TTTGAAGGTT 361 TTGTAGGTCT TACAACAAAC CCTATTCAGC CTTGTATTAG GCATGTTACA GAACCAACGA 421 ATTCGGAGAT GAAGTCAGGT CTTCCAGTTC AGCCTGCGAG GAAGACAGGT GATCCGAATC 481 CTAAGAATGC AAAAGATGGG CCGGGTGTGG TGGCTCATGC CTGTAATCCC AGCGCTTTGG 541 GAGGCCGAGG CAGGCAGATC ACCTGAGGTC GGGAGGTTGA GACCAGACTG ACCAACAACG 601 GAGAAACCCC GTCTCTACTT AAAAATGCAA AGTTAGCCGT GCGTGGTGGC CCATGCCTGT 661 ATTCCCAGCT ACTCGGGAGG CTGAGGCAGG AGAACCACTT GATCCCTGGA GGCGGAAGTT 721 GCGGTGAGCG GAGATTGCGC CATTGCACAC CAGCCCGGGC CACAAGAGCG AAACTCCGTC 781 TCAAAAAAAA AAGCAAAAGA TACTACCAAG CCCTGCGGAG CAAGGTACCT CACACTTCAT 841 GAGCGAGTTA AGATGGGTTT CACAATTTTT CAAGCAAGGA AACGGGCTCG GAGGTCTTGA 901 ACACCTGCTA CCCAATAGCA GAACAGCTAC TGGAACTAAA ATCCTCTGAT TTCAAATAAC 961 AGCCCCGCCC ACTACCACTA AGTGAAAGTC ATCCACAACC ACACACCGAC CACTCTAAGC 1021 TTTTTGTAAG ATCGGCTCGC TTTGGGGGAC AGGTCTTGAG AAGACAATCC CTTTTAAGTC 1081 AGGACCAACG TAATTTTCAT AGCTCCAAGT TCGTGTCCGA GCTGTCTAAT CCTGTAATCC 1141 AGATTGGAAA GC //
Primer: pGL4-136R () Region: Luc,insert3' Sequence file: RDB07691_A7Jjb.seq
>07691_07691_A7Jj_pGL4-136R_E01_05_ABI08.ab1 1 GCCTTATGCT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG 61 GCCGCCGAGG CCAGATCTTG ATATCTGTCC CCTCACGCTT CTCCCGGCGG CGACCCACCG 121 CCGCAAAAGA CACCCGAGGC GCAGCAGAGG CGCAGCAGTG CCACAGCGGC TCCGCCTCTG 181 CCGCCTAGTT TCAGAAGCTC GCGCCACAAG CCCGCGCTGG CCACGTGACG CCGTGACGCG 241 ACACGCAGCA CACGCACCAC CCGGAAGTCA GGCCTGGGTG GGCCGCTGCG GGTATTTCTC 301 AGTGTGGCGA AAGGAAATTC CTTGTCAGGC ATCCCAACCG TCAGTATCTG ACCGAATAAA 361 GCGGGAGCTG GAGTGTGTGT TCTCTTCTCG CTGACCCTTA AGAGTCCCAG GCCACCCTGC 421 TTGGAGGGGC CGCCCCTATG CCTACTCCAA GTCCCCCATC CCTTTCTTTT TGCTCCAGCT 481 CATGTTTGGG TGGGCGCCCT CGGGACACGA CCTGGGACCT ATGAAATACC TTTGTTCTGA 541 CCTTAAAAGG GATGTTCTCT CAAGACCTGT TCCCCAAAGC GAGCCGATCT TACAAAAGCT 601 TAGAGTGGTC GGTGTGTGGT TGTGGATGAC TTCACTTAGT GGTAGTGGGC GGGGCTGTTA 661 TTTGAAATCA GAGGATTTTA GTTCCAGTAG CTGTTCTGCT ATTGGGTAGC AGGTGTTCAA 721 GACCTCCGAG CCCGTTTCCT TGCTTGAAAA ATTGTGAAAC CCATCTTAAC TCGCTCATGA 781 AGTGTGAGGT ACCTTGCTCC GCAGGGCTTG GTAGTATCTT TTGCTTTTTT TTTTTTGAGA 841 CGGAGTTTCG CTCTTGTGGC CCGGGCTGGT GTGCAATGGC GCAATCTCCG CTCACCGCAA 901 CTTCCGCCTC CAGGGATCAA GTGGTTCTCC TGCCTCAGCC TCCCGAGTAG CTGGGAAATA 961 CAGGCATGGG CCCACCACGC ACGGCTAACT TTGCATTTTT TAAGTAGAGA CGGGTTTCTC 1021 CCGTTTGTTG GGTCAGTCTG GTCTCAACCT CCCGACTCAG TGATCTGCCC TGCTTCACTT 1081 CCAAGCGCTT GGGGAATTAC AGGCAATGAA GCCAGTCAAC TGCCCATCCC TATGGC //
Primer: pAxCALNLF1 () Region: Luc Sequence file: RDB07691_A7Jjc.seq
>07691_07691_A7Jj_pAxCALNL_F1_F01_06_ABI08.ab1 1 AAAATGGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA 61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC 121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT 181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT 241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC 301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG 361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG 421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG 481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA 541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC 601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT 661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA 721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC 781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG 841 TGTGGGCACC AGCAGGGGCA GATTGAATCT TATAGTCTTG CAAGCTGCGC AAGAATAGCT 901 CCTCCTCGAA GCGGGTACAT GAGCACGACC CGAAAGCCGC AGATCAAGTA GCCCAGCGTG 961 GTGAACATGC CGAAGCCGTG GGTGAAATGG GCACCACGCT GAGGATAGCG GGTGTCGGGG 1021 ATGATCTGGG TTTGCGAAGA TGGGGGTCGC GGGCAATGAC TGATCGGACA CAAGCGGTGC 1081 GATGCGGTTA AGGACTACGC CTCTTTGGGG CCAATTCCTG GGCTAACTGT //