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pGL4-phAPP (#RDB07692)

Promoter collection, Human APP promoter

Alternative name U3080-14
Clone info. PCR amplified human APP promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 26172063 to 26170592(NC_000021.9); relatively -1293 to +179, where +1 corresponds to 1 nt of NM_000484.4.
Comment PCR cloning, forward primer: cctagtgcgggaaaagacac; reverse primer: agcaggagcagtgccaaac. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human amyloid beta (A4) precursor protein genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07692.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07692 pGL4-phAPP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phAPP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07692).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07692_A4Lm.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07692_A4Lma.seq check
>07692_07692_A4Lm_pGL4-4174F_A08_02.ab1
    1 GCTGGGTCAG ACATTTCTCT GGCCTACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
   61 CCTAGTGCGG GAAAAGACAC ACAAGACAAC ATTTAGGGGA GCTGGTACAG AAATGACTTC
  121 CCAGGAAGGA AGTCTGTACC CCGCTGGCTG AGCCATCCTT CCCGGGCCTA GGCACCCTTG
  181 TCAGCGCAAT GAGCAAGGGA GAGAAGGCAG GCTGCAGTGC AGCCCTCAGA AGGGCCAGAG
  241 CACTCCCTGG CTTCAGTCCT TCGCTCCAAG CCCTGTGTGG AGTGGGCTGT GGCTTGGTAA
  301 CTAAATGCTA CTTCAGGTCA AGAGCAGGGG ATACATCTGG GCAGTTCTAG AGCATTCTAA
  361 ACTATCTGGA CACTAACTGG ACAGTGGACG GTTTGTGTTT AATCCAGGAG AAAGTGGCAT
  421 GGCAGAAGGT TCATTTCTAT AATTCAGGAC AGACACAATG AAGAACAAGG GCAGCGTTTG
  481 AGGTCAGAAG TCCTCATTTA CGGGGGTCGA ATACGAATGA TCTCTCCTAA TTTTTCCTTC
  541 TTCCCCAACT CAGATGGATG TTACATCCCT GCTTAACAAC AAAAAAAGAC CCCCCGCCCC
  601 GCAAAATCCA CACTGACCAC CCCCTTTAAC AAAACAAAAC CAAAAACAAA CAAAAATATA
  661 AGAAAGAAAC AAAACCCAAG CCCAGAACCC TGCTTTCAAG AAGAAGTAAA TGGGTTGGCC
  721 GCTTCTTTGC CAGGTCCTGC GCCTTGCTCC TTTGGTTCGT TCTAAAGATA GAAATTCCAG
  781 GTTGCTCGTG CCTGCTTTTG ACGTTGGGGG TTAAAAAATG AGGTTTTGCT GTCTCAACAA
  841 GCAAAGAAAA TCCTATTTCC TTTAAGCTTC ACTCGTTCTC ATTCTCTTCC AGAAACGCCT
  901 GCCCCACCTC TCCAAACCGA GAGAAAAAAC GAAATGCGGA TAAAAACGCA CCCTAGCAGC
  961 AGTCCTTTAT ACGACACCCC CGGGAGGGCC TGCGGGGTCG GATGATTCAA GCTCACGGGG
 1021 ACGAGCAGGA GCGCTCTCGA CTTTTTCTAG AGCCTCAGCG TCCCTAGGAC TCACCTTTCC
 1081 CTGATCCCTG CACCGTCCCT CTCCTGACCC AGACTCCTCC CCTCCCACTG TTCACGAGCT
1141 CAGGTGGGCC GTCGGCTCGG GGGGAAGCCG GAAGGGGGGC GCCCGCATTG TG
//
Primer: pGL4-136R
Sequence file: RDB07692_A4Lmb.seq check
>07692_07692_A4Lm_pGL4-136R_B08_05.ab1
    1 ACTTTTTGAT TTGGCATCTT CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG
   61 CCGCCGAGGC CAGATCTTGA TAGCAGGAGC AGTGCCAAAC CGGGCAGCAT CGCGACCCTG
  121 CGCGGGGCAC CGAGTGCGCT GCTGTGCGAG TGGGATCCGC CGCGTCCTTG CTCTGCCCGC
  181 GCCGCCACCG CCGCCGTCTC CCGGGGCCCC CGCGCACGCT CCTCCGCGTG CTCTCGCCTA
  241 CCGCTGCCGA GGAAACTGAC GGAGCCCGAG CGCGGCGGCG GGGCTCAGAG CCAGGCGAGT
  301 CAGCTGATCC GGCCCACCCC GCTCGGCACC CGAGAGAGAC CCCTAGCGGC GCCGCCGGGG
  361 AACTGCGCCC GCTCGCGCCG GGAGGGGCCC TCGCGCCCCG CGCCCACAGG TGCAGCGCCC
  421 TTGGCGCCGC CTGCACCCCA CGCGCCCCCT CCGCTCCCCG GCCGACGGCC CACCTGGGCT
  481 TCGTGAACAG TGGGAGGGAG AGTCTGGGGC CAGGAGAGGG ACGGTGCAGG ATCAGGGAAA
  541 GGTGAGTCCT AGGACGCTGA GGCTCTAGAA AAGTCGAGAG CGCTCCTGCT CGTCCCCGTG
  601 AGCTTGAATC ATCCGACCCC GCAGGCCTCC CGGGGGTGTC GTATAAAGGA CTGCTGCTAG
  661 GGTGCGTTTT TATCCGCATT TCGTTTTTTC TCTCGGTTTG GAGAGGTGGG GCAGGCGTTT
  721 CTGGAAGAGA ATGAGAACGA GTGAAGCTTA AAGGAAATAG GATTTTCTTT GCTTGTTGAG
  781 ACAGCAAAAC CTCATTTTTT AACCCCCAAC GTCAAAAGCA GGCACGAGCA ACCTGGAATT
  841 TCTATCTTTA GAACGAACCA AAGGAGCAAG GCGCAGGACC TGGCAAAGAA GCGGCCAACC
  901 CATTTACTTC TTCTTGAAAG CAGGGTTCTG GGCTTGGGTT TTGTTTCTTT CTTATATTTT
  961 TGTTTGTTTT TGGTTTTGTT TTGTTAAAGG GGGTGGTCAG TGTGGATTTT GCGGGGCGGG
 1021 GGGGTCTTTT TTTTGTGTTA AGCAGGGATG TACATCATCT GAGTTGGGGG AAGAAGGAAA
 1081 AATAGAGAGA TCATTTCGTA TTTCGAACCC CCGGTAAATG AAGACTTCCT GGACCTCAAA
1141 CGGCTTACCC TTGAATTCCT TTTCCAAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles