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CS-CDF-EG-PRE-RfA (#RDB07722)

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.

Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.
Comment Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging.
Vector backbone CS-CDF-EG-PRE-RfA (Plasmid)
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07722 CS-CDF-EG-PRE-RfA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-CDF-EG-PRE-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07722).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16922_A9Aop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-_C_F
Sequence file: RDB16922_A9Aoa.seq check
>07722_16922_A9Ao_7_EF1a-_C_F_A06_01_ABI08.ab1
    1 TTTCTTGGGG AATTTGCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
   61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAC ACGCTACCGG TCGCCACCAT
  121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG
  181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG
  241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT
  301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA
  361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT
  421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT
  481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA
  541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG
  601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA
  661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA
  721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT
  781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG
  841 ACTCAGATCC TACTAGTAGG ATCTCGAGGG ATCAAGCTTA TCGATAATCA ACCTCTGGAT
  901 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT
  961 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC
 1021 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGC
 1081 AACGTGCGTG GTGTGCACTG TGTTGCTGGA CGCAAACCCC CCACTGGGTT GGGCATTGCA
1141 ACCACTGGTC AGCTCCTTTT CCGAACTTCG CATTTTCCCC TCCCTATTGG CCCACCGAC
//
Primer: Cm-M_R
Sequence file: RDB16922_A9Aob.seq check
>07722_16922_A9Ao_7_Cm-M_R_B06_02_ABI08.ab1
    1 GGCTTGGGGT GAACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAATTC
   61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT
  121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT
  181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC
  241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT
  301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC
  361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA
  421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT
  481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT
  541 TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT
  601 GGTCTTAAAG GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT
  661 AGCCAGGCAC AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT
  721 TCTAGGTCTC GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA
  781 GGGCGAAGGC GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAGG
  841 TCCGCTGGAT TGAGGGCCGA AGGGACGTAG CAGAAGGACG TCCCGCGCAG AAATCCAGGT
  901 GGCAACCACA GGCGAGCAGC CATGGGAAAG GACGTCAGCT TCCCTCGACA ACACCACGGG
  961 AATTGTCAGT GGCCCAACAG CCGAGCCCCC TGTCCAGCAG CGGGGCAGGC AAGCGGCGAT
 1021 GAGTTCGCCG TGGCATAGGG GAGGGGGGAA AGCGAAAGTC CCGGAAGGGA GCTGACAGGT
 1081 GGTGGCAATG CTCCAACCAG TGGGGGTGCC GTCACCAAAC CAGTGCAACC ACGGCACGTT
 1141 GGCCTGACAA CGTGCACAAC CTCCTCATAA AGAGACAAGC CACCAGGAAT TTATCCAGGG
1201 AGGGACAAAG TGAAGAGTGC GTGCTGGGTA GGTCAT
//
Primer: BGH_rev
Sequence file: RDB16922_A9Aoc.seq check
>07722_16922_A9Ao_7_BGH_rev_C06_03_ABI08.ab1
    1 CCTTAAAAGG GGGGCCTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT GAGGGATCTC
   61 TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGCTT
  121 TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG
  181 TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC GGGCCATGTG ATGAAATGCT
  241 AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG GGTCATCCAT CCCATGCAGG
  301 CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG CTTCTTCTAC CTTCTCTTGC
  361 TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG GATATCACCA CTTTGTACAA
  421 GAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA
  481 TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGTCGACCTG
  541 CAGACTGGCT GTGTATAAGG GAGCCTGACA TTTATATTCC CCAGAACATC AGGTTAATGG
  601 CGTTTTTGAT GTCATTTTCG CGGTGGCTGA GATCAGCCAC TTCTTCCCCG ATAACGGAGA
  661 CCGGCACACT GGCCATATCG GTGGTCATCA TGCGCCAGCT TTCATCCCCG ATATGCACCA
  721 CCGGGTAAAG TTCACGGGAG ACTTTATCTG ACAGCAGACG TGCACTGGGC CAGGGGGATC
  781 ACCATCCGTC GCCCGGGCGT GTCAATAAAT ATCACTCTGT ACATCCACAA ACAGACGATA
  841 ACGGGCTCTC TCTTTTATAG GTGTAAACCT TTAAACTGCA TTTCACCAGC CCCTGTTCTC
  901 GTCAGCAAAG AGCCGTTCAT TTCAATAAAA CCGGGCGACC TCAGCCCATC CCTTCCTGAT
  961 TTCCGCTTTC CAGCGTTCGG CACGCAGACG ACGGGCTTTC ATTCTGCCAT GGTTGTGCTT
 1021 ACCCAGACCG GAGAATATTG ACATTCATAT ATTGCCCTTG AGCACTGGAT AGCCTGTCGC
 1081 TGTCCAACTG TACACTGTAA TACCGCTTGC TTCAATAGCA ATACCCTCCT TTTTGACCAT
 1141 ACTTCGGGGT ATACATAATC CAGGTATTAT AATTCTTATT AGCGCGCCAA AAAATTCGAG
 1201 CGCGGCGCCA ATTACGCGCA TAACTGGTTT ATCTCTGGGG GCCTTTTGTA GAAGTTAAAC
1261 GCAGCCAGCA G
//
Primer: CMV-Forward
Sequence file: RDB16922_A9Aod.seq check
>07722_16922_A9Ao_7_CMV-Forward_D06_04_ABI08.ab1
    1 GCGGTCAACC TCCGGCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG
  121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC
  181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT
  241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG
  301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG
  361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG
  421 AGATGGGGCG AGAGCGTCGT ATTAGCGGGG GAAATTAATC GCGATGGGAA AAATCGGTAG
  481 GGCAGGGGAA AAAAAATATA ATTAAACTAT AGTATGGCCA GCCGGGAGCA GAACGATTGC
  541 GTTAACCTGG CCGGTAAACC ACCAAAGGGT TAACAAATTT GGGAAGTAAC AACTCCCTTC
  601 AAAGGGCAAA AAAATTTATT TTTTTTTAAA ACCGGGCCCC CCCCTTTTTT GGGGGGCCCA
  661 AGGGGGAAAA AAAAAAAAAC CCCCCGGGGG TTTTTTTTCC CCATTTTGGG GGAAAGCAAA
  721 CCCATTTAAA CCCCCCCCCA AAAAAGGGGG CGCCCTTTTT TTTTTAATTT GGGGGGGGGG
  781 AAATTTGGGG GGCCATTTTG GGAAAGAATT TTTTTTTAAT ATTAAAAGGG AAAAATTGTC
  841 CCCCTTGGGG GGGCCCCCCC CCCCCCGGAA AAAAAAAAAA AGGGGGAAAA AAAAAAAAAA
  901 AGGGGGGGAA AAAGGGTTTT TTCTTTGGTG TGTGGGGGAA AAAAAAAAAA TTTGGGGGGG
  961 CATAAAATTA ACACAAGAGA GAGAAACAAA ATAAAAATGG GGGGGGGGAG TGCGCCAAAA
1021 AAATTTTTTT TGGGTGTTTT TTGTAGGGGC ACCCCCCTCT TCTTT
//
Primer: SV40pro_F
Sequence file: RDB16922_A9Aoe.seq check
>07722_16922_A9Ao_7_SV40pro_F_E06_05_ABI08.ab1
    1 CCCTGGCCCT GGACCTTTTC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG TGTTGACAAT
  121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
  181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
  241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
  301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
  361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
  421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
  481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
  541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
  601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
  661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
  721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
  781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCCTCTA GCTAGAGCTT
  841 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
  901 ACAACATACG AGCCGGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA
  961 CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAAG TCGGGAAACC TGTCGTGGCA
 1021 GGCTGCCATT AAATGGAATC GGGCCAAACG CCGCGGGGAA GAGGCGGTTT GCCGTAATGG
 1081 GGCGCTTCTT CCGCTTCCCT CGCTCACTGA CTCCGCTGCC GCTCGGTCCG TTCGGCTTGC
 1141 GGGCGTAGCG GTATACCAGC CTCACTTCAA AAGGGCGTTA ATACGGGTCA TCCCACGGAA
1201 TTCAGGGATA CCGCAGGGAA AGAAACCATG TGTGAAGCTC AACAT
//
Primer: EF1a-N_R
Sequence file: RDB16922_A9Aof.seq check
>07722_16922_A9Ao_7_EF1a-N_R_B07_02_ABI08.ab1
    1 TCCCCGGGGG GGGAATGTGG GCGATGTGCG CTCTGCCCAC TGACGGGCAC CGGAGCCTCA
   61 CGAAGCTTGA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT
  121 TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT
  181 TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC
  241 TGCCATTTGT AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT
  301 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG
  361 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC
  421 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG
  481 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT
  541 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAATTGT
  601 TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC AGAGATTTAT
  661 TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA GAGCAACCCC
  721 AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT CTTGCCTGGA
  781 GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCCTCA ATAGCCCTCA
  841 ACAAATTGTT CTGCTGCTTG CACTATACCA GACAATAATT GTCTGGGCCT GTACCGTCAG
  901 CGTCATTGAC GCCTGCGCCC ATAGTGCTTC CTTGCTGCTT CCCAAGAAAC CCAAGGAACA
  961 AGCTCCTATT CCCACTGCTC TTTTTTTCTC TCTGCACCAT CTTCTCTTGG CTTGGGTGGG
1021 GTGGCTACTT CAATGGGTTC AATTTTTTAC TACCTTCTTG TTTTATATAA ATTC
//

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References

Original, user report and related articles

user_report Kusakari, S., Shp2 in forebrain neurons regulates synaptic plasticity, locomotion, and memory formation in mice. Mol. Cell. Biol. 35 (9): 1557-1572 (2015). PMID 25713104. [link to RRC of NBRP]
user_report Maruyama, T., Hypothermia-induced tyrosine phosphorylation of SIRP alpha in the brain. J. Neurochem., 121 (6): 891-902 (2012). PMID 22468987. [link to RRC of NBRP]