RDB07825 - pGL3-AID R1 del.-02

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pGL3-AID R1 del.-02 (#RDB07825)

Reporter construct of mouse Aicda locus, position -1223 to +101.

Clone info.	Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -1223 to +101.
Comment	Nat. Immunol. (2010) 2, 148-154, Fig. 1a.
Vector backbone	PGL3 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Mouse Aicda genomic DNA
Depositor\|Developer	Honjo, Tasuku \|

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07825	pGL3-AID R1 del.-02	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID R1 del.-02 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07825).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07825_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07825_A4Gia.seq
>07825_07825_A4Gi_pGL4-4174F_H10_22.ab1 1 AGCAGGGTTC AGACATTTCT CTATCGATAG GTACCGAGCT CTTACGCGTG CTAGCCCGGG 61 CTCGAGCTGC CAGGGAGTAA GCCAAGGCCT GAACTATGAG ACTTTGCACA GAGAGATTTC 121 ACAGCAACAA AATAGGGGCA GGGGCATGTG CTGTGTGCAT GCAACGGGAT CCAGTCTCTA 181 GCTCAAGACT GGTCTGGTCT ATATAGAAAG TTCCAGACCA GCCAGAGGAG CTACATAATG 241 ACACCCTATT TTAAAAAAGG AAGGGAAGGA AGGAAGGAAG GAAGGAAGGA AGGAAAGAAG 301 GAAGAAGGAA GGAAGAAGGG AGGGAGGGAG GGAGGGAGGG AGGGAGGGAG GAAGGAAGGG 361 AGGGAGGGAG GGAGGGAGGG AGGGAGAGAG GGAAGGAGGG AGGGAGAGAG AGGGGAGGAA 421 GGAAGAAGGA AGGAAGGAAG AAAGGAAGGA AGGGAGGGAG GAAAGAAGGA AGGAAGGGAG 481 GGAGGAAAGA AGGAAGGAAG GAAGGAAGGA AGGAAGGAAG GAAGGAAGGA AGGAAGGAAG 541 GAAGGACGGA ATCAAATGAC GCCCTTCCAG ATGATCACAG CTCATATCCC AGCCAGCCAG 601 CAGGAGGCTA CTCACCCGCC TGCTCATCCT CTGCCA //
Primer: Luc_R Sequence file: RDB07825_A4Gib.seq
>07825_07825_A4Gi_Luc_R_A11_02.ab1 1 GTCTGCGCAG GACAGGGCGT ATCTCTTCAT AGCCTTATGC AGTTGCTCTC CAGCGGTTCC 61 ATCTTCCAGC GGATAGAATG GCGCCGGGCC TTTCTTTATG TTTTTGGCGT CTTCCATGGA 121 TCGGTCTCCA GCGTGACTTT CTTGACTCCC TCAAAGTCTT AGGCCAAAGG AAGCTTGCTT 181 CAAGCAAGGC TGCTTCAGTG CTGTTGTGTG ACAGAGCACT GGCCCCTGGC ATTCAAATCT 241 GAGCTTGCTC TTGTGGGCTC CTCCCCCACG ACAGCATCAC TACCACCAGG TATCCAACAT 301 CTGCCTCCTG GGTTGGGTCC AGTAAAGATG GGCGGGGCTG TGGTGGGCAA AGCCACACGG 361 ATTAAATGAT TGGGGGGGGG GGGCCAGTTC TGCTCATGCT TAATGTCCAA CTTCCTTCGA 421 GGCTGTTCCT CTAAAAAGCC AGCCCAGGTA TCACAGGTAA CAAAGATCAG GAGACAATAG 481 CACTGGACAC TTCTCACATT GGTATCATGA TAATGAGCTC TGTGAGTGTG TGTGTGTGTG 541 TGTGTGTGTG GTCCCGGGTA TCACACCTAA GGTCTGTGCA TACTTCCTAA TTTTAAAACT 601 ATGATAACAA GAATGATAGC AGTCATAGTG ACTTAAAGTG GCCTTAAATG GCACTTCAGT 661 TGCATCTTGT GCTTCTTGAA CTTACAGTAT CTGTTCCTTC CGTTTGTTAC ACTCAGTTTT 721 ATGCCTCAGT AGAGATGGGG GTTGACCACC ACAGCATCCA GAGAGTGAAC TTTAGCCTCC 781 CAATTCTCTA ATATATTTTT CTTTTTTTAA AATGACATTA TAAGATAGCT AGAAAGACTA 841 GTTGCTTGCA CCAAAAAGTG ATTTATTTTA ATTTTGTGTG TGTGTGTGTG TCAAAAAGTA 901 TTAAAAAAAA AAAAAAGAAC TCCTTTTCCT CATTAAAAAT ACCTTTACTG GGAGGCCCTG 961 GAGAGCTGGC CCAGTGATTA AGAGCACTTT ACTTCTATTG CAGAGGACCA GCAGGCAGTT 1021 TGAAAGCCAC CATGTGTGCT TTGCTGGATC TGACCTGATC ATCTGGAGAC ATCGATGCTC 1081 TCTCTTCGTT CCTTCCGTTA CATTACATTA CTTACGTACG TTCCGTTCCG TTCGTTGCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

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Other resources in our bank

mouse Aicda

External Database
Mouse Aicda

Reference sequence