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pGL3-AID delR2 + R4 (#RDB07847)

Reporter construct of mouse Aicda locus, position -9226 to -7585 plus +1202 to +1789.

Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -7585 plus +1202 to +1789.
Comment Nat. Immunol. (2010) 2, 148-154, Fig. 2d.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07847 pGL3-AID delR2 + R4 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID delR2 + R4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07847).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07847_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07847_A4Gia.seq check
>07847_07847_A4Gi_pGL4-4174F_A04_01.ab1
    1 ATTGGGGTCC GGAAAAATTG TGGTCTATCG ATAGGTACCC ACCACTCTCG GCTGTTCTGG
61 AACTCTCTGT AACCTCGGGC GGTATGAGTG TATGTGAGTG TGTGTATATG G
//
Primer: pGL3_RV5F
Sequence file: RDB07847_A4Gib.seq check
>07847_07847_A4Gi_pGL3_Rv5F_G03_21.ab1
    1 ACTTTCTCAA ATGTGGTAAA TCGATAAGGA TCCACTCTCC CTCACCCCTA TCAGGGACCA
   61 CATTGAAGAC CTCACACATG CTAGACAAGT AATCTGCCAC TTAATTACAT CCTGAGCCCT
  121 CAAAAAGCAA ACAGACAGAC AGACAGACAG ACAGACAGAC AGACAAACAA ACAAATGTTC
  181 ACAGGAGGCA GGCAGACAGC CTGAGCTGCT TCTGGGTTTA TAGTGAATTT TGAAACCAAA
  241 TCTGAGATCT ATGTCCTGAT GGAGAAGGGT CCGAGAGAAA TGCATGAGCA TGGCAAAATG
  301 CAAAGCAAAG ACGAGGCTGA GATTCAGGGA GAAGCAAACA AGACAGTGGA GAGACACAGG
  361 ATGGCACGGC ACGGACTGGA GCAAGGGCAG CGAGTAACTC AAGGCAGCCC TGCTACTAGG
  421 CTGGGATTAT TTTTAACCCC TTGAGTCTGG TTTGCATTGC TGGGGAAGCA GCTAAGGTTC
  481 TGCCTCAAGG AGCACAGCTG TCTCAGCAGC TGGCGATCTA CAGGTTTGGG ACACCACCTA
  541 GCAAAGTCCT CCTTACCGGG AGGGACATCC CGAGGAGAGG GAGCTGGAAA TAGGCTCCTA
  601 GCTAGAGTTG AGGGGGGATC CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT
  661 CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
  721 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG
  781 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
  841 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
  901 AGGAACCGTA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
  961 ATCACAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
 1021 GCGTTTCCCC TGGAAGCTCC CCTCGTGCGC TCTCTGTCGA CCTGCCGCTT ACGATTACCT
 1081 GTCGCTTTCT CCCTTCGGAA GCGTGCGCTT TCTCTAGCTC AGCCTGTAAG GTATTCTTCA
1141 GTTCCGAGTG TTAG
//
Primer:
Sequence file: RDB07847_A4Gic.seq check
>07847_07847_A4Gi_pGL3_Rv6R_F03_18.ab1
    1 GACATCGTCA GCGGATAGAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG
   61 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTGCT
  121 TCAAGCAAGG CTGCTTCAGT GCTGTTGTGT GACAGAGCAC TGGCCCCTGG CATTCAAATC
  181 TGAGCTTGCT CTTGTGGGCT CCTCCCCCAC GACAGCATCA CTACCACCAG GTATCCAACA
  241 TCTGCCTCCT GGGTTGGGTC CAGTAAAGAT GGGCGGGGCT GTGGTGGGCA AAGCCACACG
  301 GATTAAATGA TTGGGGGGGG GGGCCAGTTC TGCTCATGCT TAATGTCCAA CTTCCTTCGA
  361 GGCTGTTCCT CTAAACTCGA GCTGTAGCAC ACGCACACGC ACGCCCACAC CCTCTCTTAC
  421 AGATGCCGTA CACATGCCAT AGGAACAAAT ACAAAACCCT TGATTTGTAA GAAAGCCAAA
  481 TTCTCTCATT TGCCTTCTTC TAAAGGAAGG AAAGAGATCT CACTTAGTTA CAAAAACCAT
  541 TTTCCAGTCC CGGGAAATTT GGAACTGGTA CAGCGGCAAA GCAGTTTACA GGGTTTTGAT
  601 CAACTTTTAT TTTCTTCATT AACTTCTCTT TCCCCTCATT AACTTCTATT GAAAAACCAC
  661 AAGCCAACTT GGTCACATTG TTTTTAACCA AAAATACTGT GACTTGGAAA TCGAGTCGCC
  721 TGCGGCTGAG CTGAGTTTTG GACATCTGGA GTTCACCACC TCGCTTTCCC TATGAGACAC
  781 ACAGGTGAGG TTTCGTTATG GCCAGCATGA GGGGCTGGCG GTCTCTGGAA AGCCTCGGGG
  841 GGGGGGGGGG GCTGCATCCC TTGTGTCTCA AAGCCAGCAC ACTCAAAACA TGACTCCTCG
  901 GGTCACTATT TTTTGAACAC CAGCCTAAGA TTGCTTCAAA TATGAATACT GGTTGTTTTA
  961 AAGGGAATCC AAAAAGGCTT GGTGTGGGTG AAATGTGTGT GATTGCTTCA ACTCTGCATC
 1021 CCGATTTTTG TTTTTTCTCA CTTTGTGCTG GAAGGTTACT GGCATTCCCT GGATGAAAGC
1081 AATGGACGCT GACGACGAAT ACTCAGCTTC GCTGTTGACT GGCCTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]