RDB07855 - pGL3-AID R4 del.-01

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pGL3-AID R4 del.-01 (#RDB07855)

Reporter construct of mouse Aicda locus, position -9226 to -7585.

Clone info.	Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -7585.
Comment	Nat. Immunol. (2010) 2, 148-154, Fig. 3a, b, c.
Vector backbone	pGL3 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Mouse Aicda genomic DNA
Depositor\|Developer	Honjo, Tasuku \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07855	pGL3-AID R4 del.-01	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID R4 del.-01 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07855).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07855_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: R4-M_R Sequence file: RDB07855_A4Gia.seq
>07855_07855_A4Gi_R4-M_R_D04_10.ab1 1 CAGGAATCGG ATTTGTTTTT CTCACTTTGT GGCCCTGGAG GTTACTGCAT CCCTCTGGGG 61 TATGAAAAGC CATGGGACAG CCCTCAGACA CAGACCAGAA TTACTCAGCT TCTGCTGTTG 121 GGCTGCCCAG GACATTTCAA AGACCAGAGA ACTGTAAACA GGTATTTATC AAAAGCTTAG 181 GTCTACGGGG AGGTGGAGGC AGGAGGATTG AGAGTTCAAG GCCATCTTCT GCCTCGTGTT 241 GAATTCAAGG CCAGCAGGCA GGTTTGAAAG AAAAAAAGAA TTTTCAAATG GAATACTATG 301 AAATTAAAAG GCTTCAAACT GAGTGTGGTG ACTAATCCTA GCAGCCTGGA GGTTGAGGCA 361 GGAGGATTGT CAAGAGTTGG AAACCACCTT GAGCTCCATC CCAGGACCCA CATGGTAGGG 421 GGAGTGAACC AATTCATGCA AGTTGTCCAC TGACCTCTAC GCTCATACAC ACACACACTC 481 ATACACACAC CCATAATACA CACTCACACA CACTCATACA CACACCCATA CACACACTCA 541 TACACACACA CACCCACACA CACACCCATA CATACACACT CACATATACT CATACACCCT 601 CATCCCCACA CCCATATACA CACACTCACA CACACTCATA CACACACTCA TACACACACA 661 CCTCACACCC ATACATACAC ACTCACATAC ACTCATATAC CCTCACCCCC CCATATACAC 721 ACACTCACAC ACACTCATAC ACACACTCAT ACACACACTC ATACACACAC ACACCTCACA 781 CCCATACATA CACACTCACA TACACTCATA TACTCTCACC CCCCCCACAC CCATATACAC 841 ACACTCACAT ACACTCATAC AGCCCGAGGT TACAGAGAGT TCCAGAACAG CCGAGAGTGG 901 TGGGTACCTA TCGATAGAGA AATGTTCTGG CACCTGCACT TGCACTGGGG GACAGCCTAT 961 TTTGCTAGTT TGTTTTGTTT TCGTTTTGTT TTGATGGGAG AGCGTATGTT AGTACTATCG 1021 ATTCACACAA AAAAAACCCA ACACACAGAT GTAAATGAAA AAATAAGGAA TATTTTATTG 1081 CGGGCCGCTC GAAGTTACCC TTCCCGGTAC TAATATTACT TAACTTATCA TGGATAAGCT 1141 TGGGGGCCTT GGGGCCG //
Primer: pGL3_RV6R Sequence file: RDB07855_A4Gib.seq
>07855_07855_A4Gi_pGL3_Rv6R_D12_12.ab1 1 GTAAATCTTT CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCCA 61 TGGATCGGTC TCCAGCGTGA CTTTCTTGAC TCCCTCAAAG TCTTAGGCCA AAGGAAGCTT 121 GCTTCAAGCA AGGCTGCTTC AGTGCTGTTG TGTGACAGAG CACTGGCCCC TGGCATTCAA 181 ATCTGAGCTT GCTCTTGTGG GCTCCTCCCC CACGACAGCA TCACTACCAC CAGGTATCCA 241 ACATCTGCCT CCTGGGTTGG GTCCAGTAAA GATGGGCGGG GCTGTGGTGG GCAAAGCCAC 301 ACGGATTAAA TGATTGGGGG GGGGGGCCAG TTCTGCTCAT GCTTAATGTC CAACTTCCTT 361 CGAGGCTGTT CCTCTAAACT CGAGCTGTAG CACACGCACA CGCACGCCCA CACCCTCTCT 421 TACAGATGCC GTACACATGC CATAGGAACA AATACAAAAC CCTTGATTTG TAAGAAAGCC 481 AAATTCTCTC ATTTGCCTTC TTCTAAAGGA AGGAAAGAGA TCTCACTTAG TTACAAAAAC 541 CATTTTCCAG TCCCGGGAAA TTTGGAACTG GTACAGCGGC AAAGCAGTTT ACAGGGTTTT 601 GATCAACTTT TATTTTCTTC ATTAACTTCT CTTTCCCCTC ATTAACTTCT ATTGAAAAAC 661 CACAAGCCAA CTTGGTCACA TTGTTTTTAA CCAAAAATAC TGTGACTTGG AAATCGAGTC 721 GCCTGCGGCT GAGCTGAGTT TTGGACATCT GGAGTTCACC ACCTCGCTTT CCCTATGAGA 781 CACACAGGTG AGGTTTCGTT ATGGCCAGCA TGAGGGGCTG GCGGTCTCTG GAAAGCCTCG 841 GGGGGGGGGG GGGGCTGCAT TCCTGTGTCT CAGAGCCAGC ACACTCAGAC ATGACTCCTC 901 GGGTCACTAT TTTTGGACAC CAGCTAGATT GCTCAATATG ATACTGGTTG TTTTAAGGGG 961 ATCCAAAAAG GGCTTGTGTG GGTGAAATGT GGTTGTGGGA TTGCTCACTC TGCAATCCCC 1021 GATTTTGTTT TTCTCACTTG TGACCTTGAG TACTGCATCC CTCTGGGATG GAAGGCAATG 1081 GACAGCCTCG ACCCGACCGA ATACTCAGCT TCTGCCGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

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Other resources in our bank

mouse Aicda

External Database
Mouse Aicda

Reference sequence