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pGL3-AID R4 del.-05 (#RDB07859)

Reporter construct of mouse Aicda locus, position -8403 to -7585.

Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -8403 to -7585.
Comment Nat. Immunol. (2010) 2, 148-154, Fig. 3b.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07859 pGL3-AID R4 del.-05 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID R4 del.-05 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07859).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07859_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07859_A4Gia.seq check
>07859_07859_A4Gi_pGL4-4174F_C01_07.ab1
    1 GATAGGGGGC AGACATTTCT CTATCGATAG GTACCCCAGG GCCAAAAGTG AGAAAAACAA
   61 AAATCGGGGA ATGGGAAGTG AAGCAATCCA ACAACCACAT TTTCACCCAC ACAAAGCCCT
  121 TTTTGGATCC CTTTAAAAAC AACCAGTATC ATATTGAGCA ATCTAGCTGG TGTCCAAAAA
  181 TAGTGACCCG AGGAGTCATG TCTGAGTGTG CTGGCTCTGA GACACAGGAA TGCAGCCCCC
  241 CCCCCCCCCG AGGCTTTCCA GAGACCGCCA GCCCCTCATG CTGGCCATAA CGAAACCTCA
  301 CCTGTGTGTC TCATAGGGAA AGCGAGGTGG TGAACTCCAG ATGTCCAAAA CTCAGCTCAG
  361 CCGCAGGCGA CTCGATTTCC AAGTCACAGT ATTTTTGGTT AAAAACAATG TGACCAAGTT
  421 GGCTTGTGGT TTTTCAATAG AAGTTAATGA GGGGAAAGAG AAGTTAATGA AGAAAATAAA
  481 AGTTGATCAA AACCCTGTAA ACTGCTTTGC CGCTGTACCA GTTCCAAATT TCCCGGGACT
  541 GGAAAATGGT TTTTGTAACT AAGTGAGATC TCTTTCCTTC CTTTAGAAGA AGGCAAATGA
  601 GAGAATTTGG CTTTCTTACA AATCAAGGGT TTTGTATTTG TTCCTATGGC ATGTGTACGG
  661 CATCTGTAAG AGAGGGTGTG GGCGTGCGTG TGCGTGTGCT ACAGCTCGAG TTTAGAGGAA
  721 CAGCCTCGAA GGAAGTTGGA CATTAAGCAT GAGCAGAACT GGCCCCCCCC CCCAATCATT
  781 TAATCCGTGT GGCTTTGCCC ACCACAGCCC CGCCCATCTT TACTGGACCC AACCCAGGAG
  841 GCAGATGTTG GATACCTGGT GGTAGTGATG CTGTCGTGGG GGAGGAGCCC ACAAGAGCAA
  901 GCTCAGATTT GAATGCCAGG GGCCAGTGCT CTGTCACACA ACAGCACTGA AGCAGCCTTG
  961 CTTGAAGCAA GCTTCCCTTT GGGCCTAAGA CTTTGAGGGA GTCAAGAAAG TCACGCTGGG
 1021 AGACCGAATC CATGGGAAGA CGCCCAAAAA CATAAGAAAA GGCCCCGGCG CCATTTCTTA
 1081 TTCCCGCTGG AAGAATGGGA AACCGCTGGA GAAGCAACCT GGCCATAGCT ATGAAGAAGA
1141 ATACGCCTTG GTTCCTGGAA CAATTGGCCT TTTTTACCGA GA
//
Primer: Luc_R
Sequence file: RDB07859_A4Gib.seq check
>07869_07869_A4Gi_pGL4-4174F_D06_12.ab1
    1 AGCTTGGGGG CCAGGAAAAT TTCTCTATCG ATAGGTACCC CAGGGCCACA AAGTGAGAAA
   61 AACAAAAATC GGGGAATGCA GAAGTTGAAG CAATCCAACA ACCACATTTT CACCCACACA
  121 AAGCCCTTTT TGGATCCCTT TAAAAACAAC CAGTATCATA TTGAGCAATC TAGCTGGTGT
  181 CCAAAAATAG TGACCCGAGG AGTCATGTCT GAGTGTGCTG GCTCTGAGAC ACAGGAATGC
  241 AGCCCCCCCC CCCCCCGAGG CTTTCCAGAG ACCGCCAGCC CCTCATGCTG GCCATAACGA
  301 AACCTCACCT GTGTGTCTCA TAGGGAAAGC GAGGTGGTGA ACTCCAGATG TCCAAAACTC
  361 AGCTCAGCCG CAGGCGACTC GATTTCCAAG TCACAGTATT TTTGGTTAAA AACAATGTGA
  421 CCAAGTTGGC TTGTGGTTTT TCAATAGAAG TTAATGAGGG GAAAGAGAAG TTAATGAAGA
  481 AAATAAAAGT TGATCAAAAC CCTGTAAACT GCTTTGCCGC TGTACCAGTT CCAAATTTCC
  541 CGGGACTGGA AAATGGTTTT TGTAACTAAG TGAGATCTCT TTCCTTCCTT TAGAAGAAGG
  601 CAAATGAGAG AATTTGGCTT TCTTACAAAT CAAGGGTTTT GTATTTGTTC CTATGGCATG
  661 TGTACGGCAT CTGTAAGAGA GGGTGTGGGC GTGCGTGTGC GTGTGCTACA GCTCGAGTTT
  721 AGAGGAACAG CCTCGAAGGA AGTTGGACAT TAAGCATGAG CAGAACTGGC CCCCCCCCCC
  781 AATCATTTAA TCCGTGTGGC TTTGCCCACC ACAGCCCCGC CCATCTTTAC TGGACCCAAC
  841 CCAGGAGGCA GATGTTGGAT ACCTGGTGGT AGTGATGCTG TCGTGGGGGG AGGAGCCCAC
  901 AAGAGCAAGC TCAGATTTGA ATGCCAGGGG CCAGTGCTCT GTCACACAAC AGCACTGAAG
  961 CAGCCTTGCT TGAAGCAAGC TTTCCTTTGG CCTAAGACTT TGAAGGAGTC AAGAAAGTCA
 1021 ACGCTGGAGA CCGATCCATG GAGAACGTCA AAACATAAAG AAAGGCCGCG CCCATTCTAT
 1081 CGGCTTGAAG ATGAACCGCT GGAAAGCAAC TGCATAGGCT ATGAGAGAAT ACGCCCTGGT
1141 TTCCGTGGAA CCAAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]