RDB07860 - pGL3-AID R4 del.-06

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pGL3-AID R4 del.-06 (#RDB07860)

Reporter construct of mouse Aicda locus, position -8282 to -7585.

Clone info.	Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -8282 to -7585.
Comment	Nat. Immunol. (2010) 2, 148-154, Fig. 3b.
Vector backbone	pGL3 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Mouse Aicda genomic DNA
Depositor\|Developer	Honjo, Tasuku \|

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07860	pGL3-AID R4 del.-06	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID R4 del.-06 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07860).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07860_A4Gi.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07860_A4Gia.seq
>07860_07860_A4Gi_pGL4-4174F_E01_13.ab1 1 AATAGGTCAG ACATTTCTCT ATCGATAGGT ACCATTGAGC AATCTAGCTG GTGTCCAAAA 61 ATAGTGACCC GAGGAGTCAT GTCTGAGTGT GCTGGCTCTG AGACACAGGA ATGCAGCCCC 121 CCCCCCCCCC CGAGGCTTTC CAGAGACCGC CAGCCCCTCA TGCTGGCCAT AACGAAACCT 181 CACCTGTGTG TCTCATAGGG AAAGCGAGGT GGTGAACTCC AGATGTCCAA AACTCAGCTC 241 AGCCGCAGGC GACTCGATTT CCAAGTCACA GTATTTTTGG TTAAAAACAA TGTGACCAAG 301 TTGGCTTGTG GTTTTTCAAT AGAAGTTAAT GAGGGGAAAG AGAAGTTAAT GAAGAAAATA 361 AAAGTTGATC AAAACCCTGT AAACTGCTTT GCCGCTGTAC CAGTTCCAAA TTTCCCGGGA 421 CTGGAAAATG GTTTTTGTAA CTAAGTGAGA TCTCTTTCCT TCCTTTAGAA GAAGGCAAAT 481 GAGAGAATTT GGCTTTCTTA CAAATCAAGG GTTTTGTATT TGTTCCTATG GCATGTGTAC 541 GGCATCTGTA AGAGAGGGTG TGGGCGTGCG TGTGCGTGTG CTACAGCTCG AGTTTAGAGG 601 AACAGCCTCG AAGGAAGTTG GACATTAAGC ATGAGCAGAA CTGGCCCCCC CCCCCAATCA 661 TTTAATCCGT GTGGCTTTGC CCACCACAGC CCCGCCCATC TTTACTGGAC CCAACCCAGG 721 AGGCAGATGT TGGATACCTG GTGGTAGTGA TGCTGTCGTG GGGGAGGAGC CCACAAGAGC 781 AAGCTCAGAT TTGAATGCCA GGGGCCAGTG CTCTGTCACA CAACAGCACT GAAGCAGCCT 841 TGCTTGAAGC AAGCTTCCTT TGGCCTAAGA CTTTGAGGGA GTCAAGAAAG TCACGCTGGA 901 GACCGATCCA TGGAAGACGC CAAAAACATA AAGAAAGGCC CGGCGCCATT CTATCCGCTG 961 GAAGATGGAA CCGCTGGAGA GCAACTGCAT AAAGGCTATG AAGAGATACG CCCTGGTTTC 1021 CTGGAACAAT TGCTTTTACA GATGCACATA TCGAGGTGGA CATCACTTTA CGCTGAGTAC 1081 TTCGAATGTC GTTTCGGTTG GCAGAAGCTA TGGAAACCGA TATGGCCTGG AATTACAAAT 1141 CACAGGAAGT CGGACGTATT GCCAGGGTGA AAAACT //
Primer: Luc_R Sequence file: RDB07860_A4Gib.seq
>07860_07860_A4Gi_Luc_R_F01_16.ab1 1 CTTTGATCAG GACAGGCGTA TCTCTTCATA GCCTTATGCA GTTGCTCTCC AGCGGTTCCA 61 TCTTCCAGCG GAAAGAATGG CGCCGGGCCT TTCTTTATGT TTTTGGCGTC TTCCATGGAT 121 CGGTCTCCAG CGTGACTTTC TTGACTCCCT CAAAGTCTTA GGCCAAAGGA AGCTTGCTTC 181 AAGCAAGGCT GCTTCAGTGC TGTTGTGTGA CAGAGCACTG GCCCCTGGCA TTCAAATCTG 241 AGCTTGCTCT TGTGGGCTCC TCCCCCACGA CAGCATCACT ACCACCAGGT ATCCAACATC 301 TGCCTCCTGG GTTGGGTCCA GTAAAGATGG GCGGGGCTGT GGTGGGCAAA GCCACACGGA 361 TTAAATGATT GGGGGGGGGG GCCAGTTCTG CTCATGCTTA ATGTCCAACT TCCTTCGAGG 421 CTGTTCCTCT AAACTCGAGC TGTAGCACAC GCACACGCAC GCCCACACCC TCTCTTACAG 481 ATGCCGTACA CATGCCATAG GAACAAATAC AAAACCCTTG ATTTGTAAGA AAGCCAAATT 541 CTCTCATTTG CCTTCTTCTA AAGGAAGGAA AGAGATCTCA CTTAGTTACA AAAACCATTT 601 TCCAGTCCCG GGAAATTTGG AACTGGTACA GCGGCAAAGC AGTTTACAGG GTTTTGATCA 661 ACTTTTATTT TCTTCATTAA CTTCTCTTTC CCCTCATTAA CTTCTATTGA AAAACCACAA 721 GCCAACTTGG TCACATTGTT TTTAACCAAA AATACTGTGA CTTGGAAATC GAGTCGCCTG 781 CGGCTGAGCT GAGTTTTGGA CATCTGGAGT TCACCACCTC GCTTTCCCTA TGAGACACAC 841 AGGTGAGGTT TCGTTATGGC CAGCATGAGG GGCTGGCGGT CTCTGGAAAG CCTCGGGGGG 901 GGGGGGGGGG GCTTGCATTT CCTGGTGTCT CAAGAGCCAG CACACTTCAG ACATGAACTC 961 CTCGGGTTCA CTATTTTTTG GGACACCAGC TTAGATTGGC TCAATGGTTA CCCTATCCGA 1021 ATAGAAGAAA TGGTTCTGGG CCACCTTGCA ACTTGCACTG GGGACAGCCT ATTTTGCTTA 1081 GGTTTGTTTG TTTCGGTTTG TTTTTGATGG AGAGCGTATG TTAAGTACTA TTCGGAATTC 1141 ACCACAAAAA ACCTAACAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

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Other resources in our bank

mouse Aicda

External Database
Mouse Aicda

Reference sequence