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pGL3-AID R4 del.-07 (#RDB07861)

Reporter construct of mouse Aicda locus, position -8068 to -7585.

Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -8068 to -7585.
Comment Nat. Immunol. (2010) 2, 148-154, Fig. 3b.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07861 pGL3-AID R4 del.-07 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID R4 del.-07 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07861).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07861_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07861_A4Gia.seq check
>07861_07861_A4Gi_pGL4-4174F_G01_19.ab1
    1 TGGGTCAGAA CATTTCTCTA TCGATAGGTA CCCAGGCGAC TCGATTTCCA AGTCACAGTA
   61 TTTTTGGTTA AAAACAATGT GACCAAGTTG GCTTGTGGTT TTTCAATAGA AGTTAATGAG
  121 GGGAAAGAGA AGTTAATGAA GAAAATAAAA GTTGATCAAA ACCCTGTAAA CTGCTTTGCC
  181 GCTGTACCAG TTCCAAATTT CCCGGGACTG GAAAATGGTT TTTGTAACTA AGTGAGATCT
  241 CTTTCCTTCC TTTAGAAGAA GGCAAATGAG AGAATTTGGC TTTCTTACAA ATCAAGGGTT
  301 TTGTATTTGT TCCTATGGCA TGTGTACGGC ATCTGTAAGA GAGGGTGTGG GCGTGCGTGT
  361 GCGTGTGCTA CAGCTCGAGT TTAGAGGAAC AGCCTCGAAG GAAGTTGGAC ATTAAGCATG
  421 AGCAGAACTG GCCCCCCCCC CCAATCATTT AATCCGTGTG GCTTTGCCCA CACGGGCAAA
  481 AAAATCCCCT CTCGCCCCCC CGGTGGCCGC CCGGCCAGAA ATTTTTTTTT TTTTTTTTTT
  541 TTTTTTTTTT GGGGTTGGGG GGGGGGGGGC CCCCCCCCCC CCCCCCCAAA AAAAAAAAAA
  601 AAAAAAAGAG AAAGTTAATT TCCCCCCCCC CCTCCCCGGG GGGGAGGGGG TTTTTTTTTT
  661 TTTTTTCTCT GCGGGGGGGG GGGGGGGGGG GGGGGGGGCC CCCCACCAAC AACAAAACAA
  721 CAAACAAAAA AAATAAAAAT ATAAGTATTT TTTTTTTTTT TTTTGTGTGG GGGGGGGGGG
  781 GGGGGGGGGG GTGTGGGTCG TCTCTTCTCC CTCTCCCCCC CACAACACCA AACACAAACA
  841 AAACAAAAAA ACGAGACGAA GTGGAGAGGA GGCATACAAG AGGGCTGCGT GCTCTTCGTC
  901 TTTTTTTTTT TTGCATGGGG CGAGAGAAAA AAAAAGAAGA AAAAAAGAGA AGGGGTGGAG
  961 ATCAGTCATG CTGGCTCCGT CTCTACAGCA CCAACAAGAA AAACAGACCA CATGAGTACG
 1021 TGAGACGGAC TGGCGCGAGC CTGCGTCTGA TCGGATGCTT TTCGTAATGT TCCATTGTGC
 1081 TCTTACTGGA GGTTTGATTT GGGGATAGGT GGAGTTAGGA AGGGGTGGAG TAGTAGGCAG
 1141 AGGGGGAATG AGACATTCAG CATCATAAAA CGGAAATAAA ACAAGTGCAT AAATGAGCAA
1201 TAACAG
//
Primer: Luc_R
Sequence file: RDB07861_A4Gib.seq check
>07861_07861_A4Gi_Luc_R_H01_22.ab1
    1 GTTGATTCAG GGACAGGGCG TATCTCTTCA TAGCCTTATG CAGTTGCTCT CCAGCGGTTC
   61 CATCTTCCAG CGGATAGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG
  121 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTGCT
  181 TCAAGCAAGG CTGCTTCAGT GCTGTTGTGT GACAGAGCAC TGGCCCCTGG CATTCAAATC
  241 TGAGCTTGCT CTTGTGGGCT CCTCCCCCAC GACAGCATCA CTACCACCAG GTATCCAACA
  301 TCTGCCTCCT GGGTTGGGTC CAGTAAAGAT GGGCGGGGCT GTGGTGGGCA AAGCCACACG
  361 GATTAAATGA TTGGGGGGGG GGGCCAGTTC TGCTCATGCT TAATGTCCAA CTTCCTTCGA
  421 GGCTGTTCCT CTAAACTCGA GCTGTAGCAC ACGCACACGC ACGCCCACAC CCTCTCTTAC
  481 AGATGCCGTA CACATGCCAT AGGAACAAAT ACAAAACCCT TGATTTGTAA GAAAGCCAAA
  541 TTCTCTCATT TGCCTTCTTC TAAAGGAAGG AAAGAGATCT CACTTAGTTA CAAAAACCAT
  601 TTTCCAGTCC CGGGAAATTT GGAACTGGTA CAGCGGCAAA GCAGTTTACA GGGTTTTGAT
  661 CAACTTTTAT TTTCTTCATT AACTTCTCTT TCCCCTCATT AACTTCTATT GAAAAACCAC
  721 AAGCCAACTT GGTCACATTG TTTTTAACCA AAAATACTGT GACTTGGAAA TCGAGTCGCC
  781 TGGGTACCTA TCGATAGAGA AATGTTCTGG CACCTGCACT TGCACTGGGG ACAGCCTATT
  841 TTGCTAGTTT GTTTTGTTTC GTTTTGTTTT GATGGAGAGC GTATGTTAGT ACTATCGATT
  901 CACACAAAAA ACCAACACAC AGATGTAATG AAAATAAAGA TATTTTATTG CGGCCGCTCC
  961 AAGTACCTCC CGTACCTTTA ATATTACTTA CTTATCATGG TAGCTTGGGC TGGCGTAATA
 1021 GCGAAGAGGC CCGCACGATC GCCCTTCCAC CAGTTGCGCA GCCTGGATTG CGATTGCAAT
1081 GTAGCGTTAT ATTTGTTAAT CGCGTGATTT TTGTTAAATT CAGCTCCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]