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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL3-AID R4 del.-13

Reporter construct of mouse Aicda locus, position -9226 to -8223.

Catalog number RDB07867
Resource name pGL3-AID R4 del.-13
Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -8223.
Comment Nat. Immunol. (2010) 2, 148-154, Fig. 3c.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor|Developer Honjo, Tasuku |
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External Database
Mouse Aicda

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 学術機関の学術研究に限る。 営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          RDB07867 pGL3-AID R4 del.-13 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07867_A4Gi.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07867_A4Gia.seq check
          >07867_07867_A4Gi_pGL4-4174F_C04_07.ab1
              1 GTTAGGGGTC CAGAACATTT CTCTATCGAT AGGTACCCAC CACTCTCGGC TGTTCTGGAA
             61 CTCTCTGTAA CCTCGGGCTG TATGAGTGTA TGTGAGTGTG TGTATATGGG TGTGGGGGGG
          121 GTGAGAGT
          //
          Primer: Luc_R
          Sequence file: RDB07867_A4Gib.seq check
          >07867_07867_A4Gi_Luc_R_B02_05.ab1
              1 GACTGTTCAG GGACCAGGGC GTATCTCTTC ATAGCCTTAT GCAGTTGCTC TCCAGCGGTT
             61 CCATCTTCCA GCGGATAGAA TGGCGCCGGG CCTTTCTTTA TGTTTTTGGC GTCTTCCATG
            121 GATCGGTCTC CAGCGTGACT TTCTTGACTC CCTCAAAGTC TTAGGCCAAA GGAAGCTTGC
            181 TTCAAGCAAG GCTGCTTCAG TGCTGTTGTG TGACAGAGCA CTGGCCCCTG GCATTCAAAT
            241 CTGAGCTTGC TCTTGTGGGC TCCTCCCCCA CGACAGCATC ACTACCACCA GGTATCCAAC
            301 ATCTGCCTCC TGGGTTGGGT CCAGTAAAGA TGGGCGGGGC TGTGGTGGGC AAAGCCACAC
            361 GGATTAAATG ATTGGGGGGG GGGGCCAGTT CTGCTCATGC TTAATGTCCA ACTTCCTTCG
            421 AGGCTGTTCC TCTAAACTCG AGCAGCACAC TCAGACATGA CTCCTCGGGT CACTATTTTT
            481 GGACACCAGC TAGATTGCTC AATATGATAC TGGTTGTTTT TAAAGGGATC CAAAAAGGGC
            541 TTTGTGTGGG TGAAAATGTG GTTGTTGGAT TGCTTCAACT TCTGCATTCC CCGATTTTTG
            601 TTTTTCTCAC TTTGTGGCCC TGGAGGTTAC TGCATCCCTC TGGGGTATGA AAAGCCATGG
            661 GACAGCCCTC AGACACAGAC CAGAATTACT CAGCTTCTGC TGTTGGGCTG CCCAGGACAT
            721 TTCAAAGACC AGAGAACTGT AAACAGGTAT TTATCAAAAG CTTAGGTCTA CGGGGAGGTG
            781 GAGGCAGGAG GATTGAGAGT TCAAGGCCAT CTTCTGCCTC GTGTTGAATT CAAGGCCAGC
            841 AGGCAGGTTT GAAAGAAAAA AAAGAATTTT CAAATGGAAT ACTATGAAAT TAAAGGCTTC
            901 AAACTGAGTG TGGTGACTAA TCCTAGCCAG CCTGGAGGTT GAGGCAGGAG GATTGTCAGA
            961 GTTGGGAAAC CACCCTTGAG CTCCATCCCC AGGACCAACA TGGGTAGGGG GGGGAGGTGA
           1021 ACCCAAATTC ATTGCCAGTT GGTCCCACTT GACCTCCTAC GCTCATTACA CACACACACC
           1081 TCATACCACA CCACCCATAA TACCCACTAG ACCACACTCA TACACCACCC CGTACCGCAC
          1141 CACCTCTTAA CCCAACCACA CCCCAAACCG CATCGTACTG ACACGTTCGA CTAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl