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pGL3-AID R4 del.-17 (#RDB07871)

Reporter construct of mouse Aicda locus, position -9226 to -8357 plus -8243 to -7585.

Clone info. Reporter construct of mouse Aicda locus with firefly luciferase. Cloned position, -9226 to -8357 plus -8243 to -7585.
Comment Nat. Immunol. (2010) 2, 148-154, Fig. 4g.
Vector backbone pGL3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Aicda genomic DNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nat. Immunol., 11, 148-154 (2010)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07871 pGL3-AID R4 del.-17 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-AID R4 del.-17 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07871).

Reference section:

Tran, T.H., Nakata, M., Suzuki, K., Begum, N.A., Shinkura, R., Fagarasan, S., Honjo, T., Nagaoka, H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12850_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB12850_A4Gia.seq check
>07871_12850_A4Gi_3_pGL4-4174F_F04_16.ab1
    1 GGTGGGTCAG ACATTTCTCT ATCGATAGGT ACCCACCACT CTCGGCTGTT CTGGAACTCT
   61 CTGTAACCTC GGGCTGTATG AGTGTATGTG AGTGTGTGTA TATGGGTGTG GGGGGGGTGA
  121 GAGTATATGA GTGTATGTGA GTGTGTATGT ATGGGTGTGA GGTGTGTGTG TGTGTATGAG
181 TGTGTGTATG AGTGTGTGTA TGAGTGTGTG TGAGTGTGTG TATATGGGGG GGTGAGG
//
Primer: Luc_R
Sequence file: RDB12850_A4Gib.seq check
>07871_12850_A4Gi_3_Luc_R_G04_19.ab1
    1 CATGATTCAG GACCAGGGCG TATCTCTTCA TAGCCTTATG CAGTTGCTCT CCAGCGGTTC
   61 CATCTTCCAG CGGATAGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG
  121 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTTTC
  181 TTCAAGCAAG GCTGCTTCAG TGCTGTTGTG TGACAGAGCA CTGGCCCCTG GCATTCAAAT
  241 CTGAGCTTGC TCTTGTGGGC TCCTCCCCCA CGACAGCATC ACTACCACCA GGTATCCAAC
  301 ATCTGCCTCC TGGGTTGGGT CCAGTAAAGA TGGGCGGGGC TGTGGTGGGC AAAGCCACAC
  361 GGATTAAATG ATTGGGGGGG GGGGCCAGTT CTGCTCATGC TTAATGTCCA ACTTCCTTCG
  421 AGGCTGTTCC TCTAAACTCG AGCTGTAGCA CACGCACACG CACGCCCACA CCCTCTCTTA
  481 CAGATGCCGT ACACATGCCA TAGGAACAAA TACAAAACCC TTGATTTGTA AGAAAGCCAA
  541 ATTCTCTCAT TTGCCTTCTT CTAAAGGAAG GAAAGAGATC TCACTTAGTT ACAAAAACCA
  601 TTTTCCAGTC CCGGGAAATT TGGAACTGGT ACAGCGGCAA AGCAGTTTAC AGGGTTTTGA
  661 TCAACTTTTA TTTTCTTCAT TAACTTCTCT TTCCCCTCAT TAACTTCTAT TGAAAAACCA
  721 CAAGCCAACT TGGTCACATT GTTTTTAACC AAAAATACTG TGACTTGGAA ATCGAGTCGC
  781 CTGCGGCTGA GCTGAGTTTT GGACATCTGG AGTTCACCAC CTCGCTTTCC CTATGAGACA
  841 CACAGGTGAG GTTTCGTTAT GGCCAGCATG AGGGGCTGGC GGTCTCTGGA AAGCCTCGCG
  901 GTGGGGGGCC TGCATTCTTG GTGTCTCAAG AGCCAGCACA CTCAGACATG ACTCCTCGAG
  961 CCCGGACTAG CACGGCGTAA GAGCTCGGTA CCAACTTCTG CATTCCCGGA TTTTTTGTTT
 1021 TTTCTCACTT TGTGTCCCTG CAGGTACTGG CATCCCTTCT GGGTATGGAA AGGCATGGAC
1081 AGTCCTCGAT GACCGGAATT ACTCAGCTTC TGCCTGATGA GCCTGC
//
Primer: R4del-M_R2
Sequence file: RDB12850_A4Gic.seq check
>RDB07871_12850_A4Gi_R4del-M_R2_3_B01_04.ab1
    1 CAGGCGGGCG GTCTCTGGAG CCTCGGGGGG GGGGGGGGGC TGCATTCCTG TGTCTCAAAG
   61 CCAGCACACT CAGACATGAC TCCTCGAGCC CGGGCTAGCA CGCGTAAAAG CTCGGTACCA
  121 ACTTCTGCAT TCCCCGATTT TTGTTTTTCT CACTTTGTGG CCCTGGAGGT TACTGCATCC
  181 CTCTGGGGTA TGAAAAGCCA TGGGACAGCC CTCAAACACA GACCAAAATT ACTCAGCTTC
  241 TGCTGTTGGG CTGCCCAGGA CATTTCAAAG ACCAAAGAAC TGTAAACAGG TATTTATCAA
  301 AAGCTTAGGT CTACGGGGAG GTGGAGGCAG GAGGATTGAG AGTTCAAGGC CATCTTCTGC
  361 CTCGTGTTGA ATTCAAGGCC AGCAGGCAGG TTTGAAAGAA AAAAAAAATT TTCAAATGGA
  421 ATACTATGAA ATTAAAAGGC TTCAAACTGA GTGTGGTGAC TAATCCTAGC AGCCTGGAGG
  481 TTGAGGCAGG AGGATTGTCA AGAGTTGGAA ACCACCTTGA GCTCCATCCC AGGACCCACA
  541 TGGTAGGGGG AGTGAACCAA TTCATGCAAG TTGTCCACTG ACCTCTACGC TCATACACAC
  601 ACACACTCAT ACACACACCC ATAATACACA CTCACACACA CTCATACACA CACCCATACA
  661 CACACTCATA CACACACACA CCCACACACA CACCCATACA TACACACTCA CATATACTCA
  721 TACACCCTCA TCCCCACACC CATATACACA CACTCACACA CACTCATACA CACACTCATA
  781 CACACACACC TCACACCCAT ACATACACAC TCACATACAC TCATATACCC TCACCCCCCC
  841 CATATACACA CACTCACACA CACTCATACA CACACTCATA CACACACTCA TACACACACA
  901 CACACCTCAC ACCCATACAT ACACACTCAC ATACACTCAT ATACTCTCAC CCCCCCCACA
  961 CCCATATACA CACACTCACA TACACTCATA CAGCCCGAGG GTTACAGAGA GTTTCCAGAT
 1021 CAGCCGAGAG TTGGTGGGTT ACCTATCGAT AAGAGAAATG TTCTGGCACC TTGCACTTGG
1081 CACTGAGACA GCTATTTTGC TAGTGTTGTT CGTTTTGTTT GATGGAAGCG ATGTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806. [link to RRC of NBRP]