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CSII-CMV-mc-Myc-IRES2-Venus (#RDB07992)

Lentivirus vector plasmid of mouse c-Myc with Venus fluorescent protein marker driven by CMV promoter.

Clone info. Lentivirus vector plasmid of mouse c-Myc with Venus fluorescent protein marker driven by CMV promoter. Note: cDNA fragment 627 to 1946 of NM_010849.4 (cds, 582 to 1946) was cloned.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-CMV-RfA-IRES2-Venus (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name Mouse Myc cDNA
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB07992z.seq
Remarks, protocol and/or map (pdf) RDB07992.pdf

Distribution information

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Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07992 CSII-CMV-mc-Myc-IRES2-Venus DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-mc-Myc-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07992).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17912_B0Akp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: myc_M_R (Pr0178)
Region: insert 5', attB1, CMV pro2, CMV en2
Sequence file: RDB17912_B0Aka.seq check
>07992_17912_B0Ak_1_myc_M_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANC TCTGGTTNNC CNTGTCTCCT CNNAGTAACT CGGTCATCAT
   61 CTCCAGCTGA TCGGCGGTGG AGAAGTTGCC ACCGCCGCCG TCATCGTCTT CCCTTGGGGA
  121 GAAGGACGTA GCGACCGCAA CATAGGATGG AGAGCAGAGC CCGGAGCGGC GGCTCGGGGA
  181 CAGGGGCGGG GTGGGAAGCA GCTCGAATTT CTTCCAGATA TCCTCACTGG GCGCGGGCGG
  241 CTGCAGCTCG CTCTGCTGTT GCTGGTGATA GAAATTCTCT TCCTCGTCGC AGATGAAATA
  301 GGGCTGTACG GAGTCGTAGT CGAGGTCATA GTTCCTGTTG GTGAAGTTCA CGTTGAGGGG
  361 CATGGTGAAG GGGGCGGCCG CGGAGCCTGC TTTTTTGTAC AAACTTGTGA TGCTAGCGGA
  421 TCTGACGGTT CACTAAACCA GCTCTGCTTA TATAGACCTC CCACCGTACA CGCCTACCGC
  481 CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT TGATTTTGGT
  541 GCCAAAACAA ACTCCCATTG ACGTCAATGG GGTGGAGACT TGGAAATCCC CGTGAGTCAA
  601 ACCGCTATCC ACGCCCATTG ATGTACTGCC AAAACCGCAT CACCATGGTA ATAGCGATGA
  661 CTAATACGTA GATGTACTGC CAAGTAGGAA AGTCCCATAA GGTCATGTAC TGGGCATAAT
  721 GCCAGGCGGG CCATTTACCG TCATTGACGT CAATAGGGGG CGTACTTGGC ATATGATACA
  781 CTTGATGTAC TGCCAAGTGG GCAGTTTACC GTAAATACTC CACCCATTGA CGTCAATGGA
  841 AAGTCCCTAT TGGCGTTACT ATGGGAACAT ACGTCATTAT TGACGTCAAT GGGCGGGGGT
  901 CGNTGGGCGG NCAGCCAGGC GGGCCATTTA NCGNAAGTTA TGTAACGCGG AANTNCNTAT
  961 ATGGGNTATG AANTAATGAC CCCGNNANTG NNNACTATTN NNNGAATTNA NTCAAAGTGA
1021 ANTNNNNNNN NNCNNGNNNN NNNNNN
//
Primer: IRES-R (Pr0037)
Region: IRES2, attB2, insert 3'
Sequence file: RDB17912_B0Akb.seq check
>07992_17912_B0Ak_1_IRES-R_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNGGGGGGGG GGAGGGAGAG GGGCGGATCC
   61 GCGGCCGCTG CAGAATTCTC GAGACCGGTA GCATCACCAC TTTGTACAAG AAAGCTGGGT
  121 CGGCGCGCCC ACCCTTTTAT GCACCAGAGT TTCGAAGCTG TTCGAGTTTG TGTTTCAACT
  181 GTTCTCGTCG TTTCCTCAAT AAGTCCTTTT CAGAGGTGAG CTTGTGCTCG TCTGCTTGAA
  241 TGGACAGGAT GTAGGCGGTG GCTTTTTTGA GGATCACTAC CTTGGGGGCC TTTTCGTTGT
  301 TTTCCAATTC AGGGATCTGG TCACGCAGGG CAAAAAAGCT GCGCTTCAGC TCGTTCCTCC
  361 TCTGACGTTC CAAGACGTTG TGTGTCCGCC TCTTGTCGTT TTCCTCCGTG TCTGAGGACC
  421 TGGGGCTGGA GCACTTGCGG TTGTTGCTGA TCTGCTTCAG GACCCTGCCA CTGTCCAACT
  481 TGGCCCTCTT GGCAGCTGGA TAGTCCTTCC TTGTGGAGGG GGGTGCGGCG TAGTTGTGCT
  541 GGTGAGTGGA GACGTGGCAC CTCTTGAGGA CCAGTGGGCT GTGCGGAGGT TTGCTGTGGC
  601 CTCGGGATGG AGATGAGCCC GACTCCGACC TCTTGGCAGG GGTTTGCCTC TTCTCCACAG
  661 ACACCACATC AATTTCTTCC TCATCTTCTT GCTCTTCTTC AGAGTCGCTG CTGGTGGTGG
  721 GCGGTGTCTC CTCATGCAGC ACTAGGGGCT CAGGGCTGGC CCGTGGGGAG GACTCGGAGG
  781 ACAGCAGCGA GTCCGAGGAA GGANANAANG NCGTGGAATC NGACGAGGTA CAGGATTTGG
  841 GCGANNNTGC TGTCGTTGAN CGGGTAGGGA AANANNACTG ANGGGTCNAT GCACTCGGAN
  901 GCNGCGGCGG NGANGTCCTG NNNNNACAGG CTGGAGGTGG ANCAGACNCT GNGCCCGCGG
  961 GCGGGGCTCN NNTGGNGNTG TCTTTNNNCN CANNNNGGTA NNNGNNCNNN TNNNNNNNNN
1021 N
//
Primer: HIV-B_R (Pr0673)
Region: HIV-1 psi, 5'LTR, CMV pro1, CMV en1
Sequence file: RDB17912_B0Akc.seq check
>07992_17912_B0Ak_1_HIV-B_R_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNNCNNNNNN NNNTTTTGGC GTACTCNCCA GTCGCCGCCC CTCGCCTCTT
   61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC
  121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC
  181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC
  241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG
  301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC
  361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT
  421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA
  481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC
  541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT
  601 AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG
  661 GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC
  721 TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT
  781 ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT
  841 TATGTAACGC GGAACTCCNT ATATGGGCTA TGAACTAATG ACNNNGNAAT TGATTACNAT
  901 TAATAACTAG TCAATAATCA ATGNCAACGC GTATATCTGG NCCGNACNTC GCGAANCANC
  961 GCAAAACGCC NNACCCTNAG CANANTCNTC NTGNNNTTGN CNGNNNNGCC TNNNCNNNNN
1021 GNNNNNNNAN NTTNGNNNGC GCNCNANTCA NCGANNNNNN NNNN
//
Primer: HIVgp_R1 (Pr0132)
Region: HIV-1 psi, 5'LTR
Sequence file: RDB17912_B0Akd.seq check
>07992_17912_B0Ak_1_HIVgp_R1_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGCTC TTTTTTCTCT CTGCACCNCT CTTCTCTTTG CCTTGGTGGG
   61 TGCTACTCCT AATGGTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT
  121 GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG CTGTGCGGTG
  181 GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT GTCTTTTATC
  241 TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA TCTAAGTTCT
  301 TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT
  361 GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA
  421 TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATCGCGAT
  481 CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT CTAGCCTCCG
  541 CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC CGTGCGCGCT
  601 TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC TTTCAAGTCC
  661 CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT
  721 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT
  781 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGANAG CTCCCAGGCT CAGATCTGGT
  841 CTAACCAGAG AGANCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC CCACCGTACA
  901 CGCCTACCGC CCATTTGCGT CAATGGGGCG GANTTGTTAC NANNTTTTGG AAANTCCCGT
  961 TGANTTTGGN NNCNAAACAA ACTCCCATTG ANGTCNATGG GGNNNNNNNN TNNNAATCCC
 1021 NNNNANNNNA ANNGNTATNN NNGNCNNNNG NNGNNNNGNN NAAANNNNAN NNNCNNNNNN
1081 NTANNNNNNN NNN
//
Primer: IRES-F (Pr0036)
Region: IRES2, Venus 5'
Sequence file: RDB17912_B0Ake.seq check
>07992_17912_B0Ak_1_IRES-F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTCTAGGC CCCCCGAACC
   61 NCGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC
  721 TACCAGTCCG CCCTGANCAA AGACCCCAAC GAGAAGCGCG ATCACNTGGT CCTGCTGGAG
  781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGANC TGTACAAGTA ANAANTCNAG
  841 GCCTCTCNAG CCTCTAGAGT TAANATCNAN GGATCAAGCT TATCGATNAT NNANNTCTGG
  901 ATNNCAAAAN TTGNNNAANA NTGACTGGTA NNCNTAACTA TGTTGCTCCT TTTACNCNNT
  961 GTGGATACNC TGCTTTAATG CCNTNGNANC ATGCTANNGC TTCCCGNANG GNTTNNNNTT
1021 TCNNNNCCTT GNNNNAAATC CNGNTNGCTG NNNNNTTNNG ANGNANNTNN NNNNNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE, Venus 3'
Sequence file: RDB17912_B0Akf.seq check
>07992_17912_B0Ak_1_PRE_rev_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNGCGNNN NGGANCNNNN TAGTTAAGAA TACCNGTCAA TCTTTCACAA
   61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG
  121 CTCGAGAGGC CTTGAATTCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
  181 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
  241 CGGACTGGTA GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
  301 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCCGCCGTC CTCGATGTTG TGGCGGATCT
  361 TGAAGTTGGC CTTGATGCCG TTCTTCTGCT TGTCGGCGGT GATATAGACG TTGTGGCTGT
  421 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
  481 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
  541 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
  601 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GGCGAAGCAC TGCAGGCCGT
  661 AGCCCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATCA
  721 GCTTCAGGGT CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT
  781 TGTGGNCGTT TACGTCGCCG TCCAGCTCGA CCAGGANGGG CACCACCCCG GTGAACAGCT
  841 CCTCGCCCTT GCTCACCATG GTTGTGNNCA TATTATCATC GTGTTTTTCN AAGGAAAANC
  901 ACGTCCCCGN GGNTCGGGGG NNCTANACGT TTTTTTAANC TCGANNNAAC ACATGTNAAG
  961 CATGTGTACC GANGCCCCNN ATNANATNCC NTACNNTGGG GGTANNTTCN GGNNNTNNNN
1021 CANCCCNNTN NNNNNNNANN NNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGHp(A), 3'LTR, PRE
Sequence file: RDB17912_B0Akg.seq check
>07992_17912_B0Ak_1_BGH_rev2_G02_20_ABI24.ab1
    1 NNNNNNNNNG NNNNNNGGGG GNNGGGCNNA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG
  121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
  181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
  241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC
  301 ATGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA
  361 TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT
  421 TCTACCTTCT CTTGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT
  481 CTGATCCCTG GCCCTGGTGT GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG
  541 ATCCACAGAT CAAGGATATC TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC
  601 TTTTCTTTTA AAAAGTGGCT AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT
  661 ACCTGAGGTG TGACTGGAAA ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA
  721 TCAGCATTGG TAGCTGCTGT ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA
  781 CGGTATCGAT GCGGGGANGC GGCCCAAAGG GAGATCCGAC TCGTCTGANG GCGAANGCGA
  841 AGANGCGGAA GANGNCGCAN AGCCGGCAGC ANGCCGCGNG AANGAANGTC CGCTGGNNTG
  901 ANGGNCGAAN GGANNTANCA NAAGGNNGTC CCGCGCANAA TNCNGGTGNN NACNCAGGCG
  961 ANCAGCCNTN NNAAGGANGT CNNCTTCCCN NANNNCNCNN NNGANNTGNN AGTGNCNNNC
1021 AGNNNANCCN NNGNNCANCA NNNGNNNNNN NNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, Zeo, SV40p(A)
Sequence file: RDB17912_B0Akh.seq check
>07992_17912_B0Ak_1_SV40pro_ori_F_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNC NCCTCTGCCT CTGAGCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
  181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
  241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
  301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
  361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
  421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
  481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
  541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
  601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
  661 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
  721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  781 ACTGCATTCT AGTTGTGGTT TGTCCNAACT CATCAATGTA TCTTATCNTG TCTGTATACC
  841 GTCGACCTCT AGCTAGAGCT TGNCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG
  901 NTATCCGCTC ACNATTNCNC ACNACATACN ANCCNGAAGC ATAAAGTGNN AAGCNNGGGG
  961 NGNCTAATGA NNGANCTANN TCNNATNNAT TGNGTTGNNC TNNCNGNCNG NTTTNCNNNC
1021 NGNNAANNNN NCNNGNNNNC NGNNNNNNNG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles