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CSII-CMV-Venus-Mito-IRES2-Bsd (#RDB08118)

Lentivirus vector plasmid of Venus fluorescent protein with mitochondria localization signal driven by CMV promoter.

Clone info. Lentivirus vector plasmid of Venus fluorescent protein with mitochondria localization signal driven by CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Selectable markers Ampicillin (E. coli), Bsd (lentivirus transfection). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB08118z.seq
Remarks, protocol and/or map (pdf) RDB08118.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08118 CSII-CMV-Venus-Mito-IRES2-Bsd Please contact us. JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-Venus-Mito-IRES2-Bsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08118).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08118_A3Ihp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Venus-N_R
Sequence file: RDB08118_A3Iha.seq check
>08118_08118_A3Ih_Venus-N_R_D09_12.ab1
    1 CCGTCTGAGC TGACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA
   61 CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGC GACCGGTGGA TCCCCCAACG
  121 AATGGATCTT GGCGCGCGGC ACTGGGAGCC GCCGGGCCGA GCCTGTCAAG CCCCGCAGCA
  181 GCAGCGGCGT CAGGACGGAC ATGCTAGCGG ATCTGACGGT TCACTAAACC AGCTCTGCTT
  241 ATATAGACCT CCCACCGTAC ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA
  301 CGACATTTTG GAAAGTCCCG TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG
  361 GGGTGGAGAC TTGGAAATCC CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC
  421 CAAAACCGCA TCACCATGGT AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA
  481 AAGTCCCATA AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG
  541 TCAATAGGGG GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCAAGTG GGCAGTTTAC
  601 CGTAAATACT CCACCCATTG ACGTCAATGG AAAGTCCCTA TTGGCGTTAC TATGGGAACA
  661 TACGTCATTA TTGACGTCAA TGGGCGGGGG TCGTTGGGCG GTCAGCCAGG CGGGCCATTT
  721 ACCGTAAGTT ATGTAACGCG GAACTCCATA TATGGGCTAT GAACTAATGA CCCCGTAATT
  781 GATTACTATT AATCGAATTA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA TAAACCCGAA
  841 AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA
  901 TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT
  961 GGATGAATAC TGCCATTTGT AGATCCGTTT CACTAATCGA ATGGATCTGT CTCTGTCTCT
 1021 CTCTCACTTT CCTCCTCTAT TCTTCCGGCT GTCAGTCCCT CGGGGTTTGG AGGTGGGTCT
1081 GAATCGATTA TGATGATATC CATGGCTACT TCTTATTTCA CTATTAGG
//
Primer: IRES-R
Sequence file: RDB08118_A3Ihb.seq check
>08118_08118_A3Ih_IRES-R_D10_10.ab1
    1 GCACGGGGGG CTTCGGGCCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
   61 CCGCTGCAGA ATTCTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC
  121 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC
  181 TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC
  241 TGCTGGTAGT GGTCGGCGAG CTGCACGCCG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG
  301 TTGGCCTTGA TGCCGTTCTT CTGCTTGTCG GCGGTGATAT AGACGTTGTG GCTGTTGTAG
  361 TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC
  421 TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT CTTGTAGTTG
  481 CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT GGCGGACTTG
  541 AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGGCGA AGCACTGCAG GCCGTAGCCC
  601 AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA GATCAGCTTC
  661 AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG
  721 CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG
  781 CCCTTGCTCA CCATGGTGGC GACCGGTGGA TCCCCCAACG AATGGATCTT GGCGCGCGGC
  841 ACTGGGGAGC CGCCGGGCCG AGCCTGTCAA GCCCCGCAGC AGCAGCGGCG TCAGGACGGA
  901 CATGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT TATATAGACC TCCCACCGTA
  961 CACGCCCTAC CGCCCATTTG CGTCAATGGG GGCGGAGTTG TTACGACATT TTTGGGAAAG
 1021 TCCCGTTTGA TTTTTGGTGC CAAACAAACT CCATGACGTC ATGGGGTGGA GACTTGGAAT
1081 TCCCCGTTGA GTCAACGGCC TATTCAACGC CCCCAA
//
Primer: IRES-F
Sequence file: RDB08118_A3Ihc.seq check
>08118_08118_A3Ih_IRES-F_H08_23.ab1
    1 GCATCACTTC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGCC AAGCCTTTGT
  121 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
  181 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
  241 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
  301 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
  361 TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
  421 CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
  481 CTGGTTATGT GTGGGAGGGC TAAGTCGAGC CTCTAGAGTT AACATCGAGG GATCAAGCTT
  541 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT
  601 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT
  661 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG
  721 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC
  781 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC
  841 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT
  901 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG
  961 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG
 1021 GCCCTCAATC CAGCGGGACC TTCTTCCCGC GGCCTGCTGC CGGCTCTGCG GGCTCTTCGC
 1081 GTCTTCGCTT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTT GGGCCGCCTC CCCGCAATCG
 1141 ATACGGTCGA GACTTAGAAA AACATGAAGC AATCACAAGT TAGCCAAATA ACAGCAGAGC
1201 CTTAACCTAA T
//
Primer: HIVgp_R1
Sequence file: RDB08118_A3Ihd.seq check
>08118_08118_A3Ih_HIVgp_R1_E10_13.ab1
    1 CGGGACCTCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
  841 GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC TCCCACCGTA
  901 CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGGAGTTG TTACGACATT TTGGAAAGTC
  961 CCGTTTGATT TTGGGTGCCA AAACAAACTC CCATTGACGT CATGGGGGTG GGAGACTTGG
 1021 GAAATCCCCG TGAGTCAAAC CGCTAATCCA CGCTCATGAT GTTACTGCCA AACCGGCATC
1081 ACAATGCTAA TAGCGAATGA CTTAATTAAC GCTAGATGGT AC
//
Primer: BGH_rev2
Sequence file: RDB08118_A3Ihe.seq check
>08118_08118_A3Ih_BGH_rev2_F10_16.ab1
    1 CCACTCCCCC GATCAGTAAG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA
  121 CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC
  181 TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA
  241 GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC
  301 TCGGGCCATG TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC
  361 CGGGTCATCC ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT
  421 GGCTTCTTCT ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG
  481 TGGATATCTG ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT
  541 GTGGTAGATC CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG
  601 TCCCCCCTTT TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT
  661 TAAAGGTACC TGAGGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA
  721 GGCACAATCA GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG
  781 GTCTCGACGG TATCGATGCG GGGAGGCGGC CCAAAGGGAG ATCCGACTCG TCTGAGGGCG
  841 AAGGCGAAGA CGCGGAAGAG GCCGCAGAGC CGGCAGCAGG CCGCGGGAAA GGAAAGGTCC
  901 GCTGGATTGA GGGCCGACGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA
  961 ACACAGGCGA GCAGCCATGG GAAAGGACGT CAGCTTCCCC CGACAACACC ACGGGAATTT
 1021 GTCAGTTGCC TCAACAGCCC GGAGCCCCCT GTCAGCCAGC CGGGCAAGCA AGCGGCGATG
1081 AGTCATCGAT GACATTAGGG AGGGGAAAGC CGAAAAGT
//
Primer: SV40pro_F
Sequence file: RDB08118_A3Ihf.seq check
>08118_08118_A3Ih_SV40pro_F_G10_19.ab1
    1 CTTTGGTCTG TGGCTTTTCA GGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
   61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAGCACG TGTTGACAAT
  121 TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG
  181 GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG
  241 TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG
  301 GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC
  361 ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC
  421 GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG
  481 TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG
  541 GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG
  601 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
  661 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
  721 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
  781 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG
  841 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAAATTGT TATCCGCTCA CAATTCCACA
  901 CAACATACGA GCCGGAAGCA TAAAGTGTAA AAGCCTGGGG GTGCCTAATG AGTGAGCTAA
  961 CTCACATTTA ATTGCGTTTG CGCTCACTGC CCGGCTTTCC AGTCGGGAAA ACCTGTCGTG
 1021 CCAAGCTGCA TTTAATGAAT CGGCCAACGG CGCGGAAGAG GCGGGTTTGC GTATTGGGCG
1081 CTCTTTCGGC CTATCCATCG CTCACCACTG GGACATTCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Ogawa, Y., Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci. Rep. 8 (1): 2722 (2018). PMID 29426875. [link to RRC of NBRP]