Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET21c_Tr-cbh2-His

Expression vector of Exoglucanase 2 of Trichoderma reesei

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08287_A6Afp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08287_A6Afa.seq
>08287_08287_A6Af_T7_20bp_E03_15.ab1     1 TCCCCAGGTT CAATTCCCCC TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC    61 ATATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGAT CCGAATTCGA GCTCCGTCGA   121 CCGTGCCTCT AGAGGAGCGG CAAGCTTGCT CAAGCGTCTG GGGCCAATGT GGTGGCCAGA   181 ATTGGTCGGG TCCGACTTGC TGTGCTTCCG GAAGCACATG CGTCTACTCC AACGACTATT   241 ACTCCCAGTG TCTTCCCGGC GCTGCAAGCT CAAGCTCGTC CACGCGCGCC GCGTCGACGA   301 CTTCTCGAGT ATCCCCCACA ACATCCCGGT CGAGCTCCGC GACGCCTCCA CCTGGTTCTA   361 CTACTACCAG AGTACCTCCA GTCGGATCGG GAACCGCTAC GTATTCAGGC AACCCTTTTG   421 TTGGGGTCAC TCCTTGGGCC AATGCATATT ACGCCTCTGA AGTTAGCAGC CTCGCTATTC   481 CTAGCTTGAC TGGAGCCATG GCCACTGCTG CAGCAGCTGT CGCAAAGGTT CCCTCTTTTA   541 TGTGGCTAGA TACTCTTGAC AAGACCCCTC TCATGGAGCA AACCTTGGCC GACATCCGCA   601 CCGCCAACAA GAATGGCGGT AACTATGCCG GACAGTTTGT GGTGTATGAC TTGCCGGATC   661 GCGATTGCGC TGCCCTTGCC TCGAATGGCG AATACTCTAT TGCCGATGGT GGCGTCGCCA   721 AATATAAGAA CTATATCGAC ACCATTCGTC AAATTGTCGT GGAATATTCC GATATCCGGA   781 CCCTCCTGGT TATTGAGCCT GACTCTCTTG CCAACCTGGT GACCAACCTC GGTACTCCAA   841 AGTGTGCCAA TGCTCAGTCA GCCTACCTTG AGTGCATCAA CTACGCCGTC ACACAGCTGA   901 ACCTTCCAAA TGTTGCGATG TATTTGGACG CTGGCCATGC AGGATGGCTT GGCTGGCCGG   961 CAAACCAAGA CCCGGCCCGC TCAGCTATTT GCAATGTTTA CAGAATGCAT CGTCTCCGAG  1021 AGCTCTTCGC GGAATTGGCA ACCCAATGTC GCCCAACTAC ACGGGGTGAC CATTTACCCA  1081 GCCCCCTCAT CGTACACGCA AGGCAACGGC TGTCTACCAC CGAGAAGCCT GGTACATTCA 1141 CGCTAATTGG ACCCTTCTTT CTTTGTGCCA ATC //
Primer: f1ori_R3 Sequence file: RDB08287_A6Afb.seq
>08287_08287_A6Af_f1ori_R3_F03_18.ab1     1 TGGGTCGCCA ATCGGAAAAG TTTCTCCTTT CAGCAAAAAA CCCCCTCAAG GACCCGTTTA    61 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAAGCGGTG GCAGCAGCCA ACTCAGCTTC   121 CTTTCGGGCT TTGTTAGCAG CCGGATCTCA GTGGTGGTGG TGGTGGTGCT CGAGTGCGGC   181 CGCAAGCTTG TCGACCAGGA ACGATGGGTT TGCGTTTGTG AGAAGCTGCA CAAAGTAGGC   241 TTGGAACCAA GCACCAGCTT GAGGCGCCGG TTGCAAGGCA TCTGGGAGCG CACAGTGGGA   301 GTCAAATCGT GGCGCACTGC TGTCGCTGGT GCCGTCACAC TCGCCGCCTG GCTTGACCCA   361 GACAAACGAA TCCAGCAACG AGTCCCCAGT GTTTGCGGAT GGGCGAATAC CAAATCCGGT   421 GCCGATCACA TTGCACCAGT CTCCCCACTG TTGCTGTCCG GTAGGCTGCT TTCCCGATCG   481 ACCTTGATCA GTGATGAAGA AGGCGTTGGA CCAGCCGTGA TTGGCAAGAA GAGGTCCAAT   541 AGCGTGGATG TACAGCTTCT CGTTGTAGAC AGCGTTGCCT TGCGTGTACG ATGGGGGGCT   601 GGTAATGTTC CACCCGTTGT AGTTGGCGAC ATTGGTTGCC AATCCGCGAA GAGCTCTCGG   661 AGACGATGCA TTCTTGTAAA CATTTGCAAA TAGCTGAGCG GCCGGGTCTT GGTTTGCCGG   721 CCAGCCAAGC CATCCTGCAT GGCCAGCGTC CAAATACATC GCAAACATTT GGAAGGTTCA   781 GCTGTGTGAC GGCGTAGTTG ATGCACTCAA GGTAGGCTGA CTGAGCATTG GCACACTTTG   841 GAGTACCGAG GTTGGTCACC AGGTTGGGCA AGAGAGTCAG GCTCAATAAC CAGGGAGGGT   901 CCCGGATATC GGGAATAATT CCACGACAAT TTGACGAATG GTGTCGATAT AGTTCTTATA   961 TTTGGGCGAC GCCACCATCG GCAATAGAGT AATTCGCCCA TTCGAGGCAA AGGGCAGCGC  1021 CAATCGCGAA TCCGGCAAGG TCATACCACA ACAACTGGTC CGGGCATTAG TTACCGCCAA  1081 TTCTGTTGGC GGGTGCCGGA TGTCGGGCAG TTTTGCTCAA TGAGAGGGGT CTTGTCAGAG 1141 TATCTAAGCT ACATAAAAGG AAAGGGGAAA CCTTTTGGGC GACACGCCGG CC //
Primer: pGL3_RV4 Sequence file: RDB08287_A6Afc.seq
>08287_08287_A6Af_pGL3_Rv4_G03_21.ab1     1 GGAGGGGCAT GAAATGAGGT TGAGGCTCTC AGGGCATCGG TCGAGATCCC GGTGCCTAAT    61 GAGTGAGCTA ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC   121 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG   181 GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC   241 GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA   301 TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT   361 CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG   421 CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC   481 ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC   541 GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG   601 ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC   661 TCCACGCCCA GTCGCGTACC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG   721 TCAGAGACAT CAAGAAATAA CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA   781 TCCTGGTCAT CCAGCGGATA GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG   841 TGCACCGCCG CTTTACAGGC TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG   901 GCACCCAGTT GATCGGCGCG AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG   961 GCCAGACTGG AGGTGGCAAC GCCCAATCAG CAACGACTGT TTGCCCGCCC AGTTTGTTGT  1021 GCCACGCGGA TGGGGAATGT AATTCAGCTC CGCCCATCGC CGCTTCCACT TTTTCCCCGC  1081 GTTTTCCGCA GAAACGTGAC TGGCTGGATT TCACCACGCG GAACCGGTCT GATAAGAGAC 1141 ACCGGCCATA CTCTTGCCGA ACAATCGTAA TTTAAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl