RDB08306 - pET25b_Tr-cbh1-His

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pET25b_Tr-cbh1-His

Expression vector of Exoglucanase 1 of Trichoderma reesei

Catalog number	RDB08306
Resource name	pET25b_Tr-cbh1-His
Alternative name	pET25b_Tr001-His
Clone info.	Biomass collection. the Exoglucanase 1 (CBH1, 1,4-beta-cellobiohydrolase Exocellobiohydrolase I (EC 3.2.1.91)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone	pET-25b (Plasmid)
Size of vector backbone	5,547 bp
Selectable markers	Amp^r
Gene/insert name	Trichoderma reesei cbh1 cDNA
Depositor\|Developer	DNA Bank, \| Kishikawa, Shotaro \|

Other clones in our bank

External Database
Trichoderma reesei cbh1

Reference sequence

Sequence	RDB08306z.seq
Remarks, protocol and/or map (pdf)	RDB08306.pdf

Distribution information

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Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

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RDB08306	pET25b_Tr-cbh1-His	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET25b_Tr-cbh1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08306).

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Biomass Collection (English text)
Featured content	Biomass Collection (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08306_A5Iip1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08306_A5Iia.seq
>08306_08306_A5Ii_T7_20bp_E12_15.ab1 1 GAAGGGATGG GTTGGGGGGT TCTAGAAATA GGGTTGTTTA CTTTAAGAAG GACGATATAC 61 ATATGAAATA CCTGCTGCCG ACCGCTGCTG CTGGTCTGCT GCTCCTCGCT GCCCAGCCGG 121 CGATGGCCAT GGATATCGGA ATTAATTCGG ATCCGAATTC GAGCTCCGTC GACCAGTCGG 181 CCTGCACTCT CCAATCGGAG ACTCACCCGC CTCTGACATG GCAGAAATGC TCGTCTGGTG 241 GCACGTGCAC TCAACAGACA GGCTCCGTGG TCATCGACGC CAACTGGCGC TGGACTCACG 301 CTACGAACAG CAGCACGAAC TGCTACGATG GCAACACTTG GAGCTCGACC CTATGTCCTG 361 ACAACGAGAC CTGCGCGAAG AACTGCTGTC TGGACGGTGC CGCCTACGCG TCCACGTACG 421 GAGTTACCAC GAGCGGTAAC AGCCTCTCCA TTGGCTTTGT CACCCAGTCT GCGCAGAAGA 481 ACGTTGGCGC TCGCCCTTTA CCTTATGGCG AGCGACACGA CCTACCCAGG AATTCACCCT 541 GCTTGGCAAC GAAGTTTCTC TTTCGATGTT GATGTTTCGC AGCTGCCGTG CGGCTTGAAC 601 GGAGCTCTCT ACTTCGTGTC CAATGGACGC GGATGGTGGC GTGAGCAAGT ATCCCACCAA 661 CACCGCTGGC GCCAAGTACG GCACGGGGGT ACTGTGACAG CCAGTGTCCC CGCGATCTGA 721 AGTTCATCAA TGGCCAGGCC AACGTTGAGG GCTGGGAGCC GTCATCCAAC AACGCGAACA 781 CGGGCATTGA GGACACGGAA GCTGCTGCTC TGGAGATGGA TATCTGGGAG GCCAACTCCA 841 TCTCCGAGGC TCT //
Primer: f1ori_R3 Sequence file: RDB08306_A5Iib.seq
>08306_08306_A5Ii_f1ori_R3_F12_18.ab1 1 GGATTCCATC GGGATTAGTT CTCCTTTCAG CAAAAACCCC TCAAGACCCG TTTAGAGGCC 61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG 121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGACATC CTCGGGGTCT 181 TCCGGGGCGA GTTCTGGCTG GCTAGCCCGT TTGATCTCGA GTGCGGCCGC AAGCTTGTCG 241 ACCAGGCACT GAGAGTAGTA AGGGTTCAGG ACCTGGCAAG TTGTGCCGCT GGCGCAGACC 301 GTGGGGCCGC TGTAGCCAAT ACCGCCGCAC TGGCCGTAGT GAGACTGGGT AGGTCCGGGA 361 GAGCTTCCAG TGGTAGTGGC TGGGCGGCGG GTGGTGGTGG TGCCAGGCGG GTTTCCGCCG 421 GGAGGGTTGC CGCCGCTAGG GTTGCCGGTG CTGCCAATGG GTCCGAACTT GATGTTGGAG 481 AAGGTGACCT TGGCGTTGGG AGACTGAGAT TCGACCTGAG CAGGGACACC GGAGCTGGTG 541 GAGCAGCTTC CGCGCACGGC ACCGGGTGTG GAGGAGGTCT CGTTTGTCGG GTAGGTGGAG 601 TCCAGCCACA GCATGTTGGC GTAGTAATCA TCCCACAGAC TCATGACCAG AACCATGCCG 661 CCAGAGGTAG CCTTCTTGAA CTGAGTCAGG CCGCCCTTGT CTGAGAAAGA GGATCCGCCG 721 AATTCTGCCT CCTCAGCTGT GCAGTAATCA TCGTTGAGCT CGTTGCCAGA GTAACTACCC 781 AGCTCGGCGT TGGGCTGCTG GAAAGTGACG CCATTCTGGA CATAGTATCG GTTGATGGGC 841 ACCCGACGTC TCGAACTGGG TGACACGGGT CAATTTCTTG TGTATCGAGG GTAAGCTGGA 901 GCCAGGCGGT AGAGCTGTGT TGCTCAAGCG GTATGGTTCA GTCGCTAGCA TCGGATCGCA 961 AGTGCCGCAT ACTGTAATCG GAGCTAAAGT TCCGGCGGCA CCATCACCTC GA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl