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pET25b_Tr-egl1-His (#RDB08308)

Expression vector of Endoglucanase EG-I of Trichoderma reesei

Alternative name pET25b_Tr003-His
Clone info. Biomass collection. the Endoglucanase EG-I (EGLI, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei egl1 cDNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |
 
Sequence RDB08308z.seq
Remarks, protocol and/or map (pdf) RDB08308.pdf

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08308 pET25b_Tr-egl1-His DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET25b_Tr-egl1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08308).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08308_A5Iip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB08308_A5Iia.seq check
>08308_08308_A5Ii_T7_20bp_A01_01.ab1
    1 TGCGGGTCAT TCCCCTCTAG AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGA
   61 AATACCTGCT GCCGACCGCT GCTGCTGGTC TGCTGCTCCT CGCTGCCCAG CCGGCGATGG
  121 CCATGGATAT CGGAATTAAT TCGGATCCGA ATTCGAGCTC CGTCGACCAG CAACCGGGTA
  181 CCAGCACCCC CGAGGTCCAT CCCAAGTTGA CAACCTACAA GTGTACAAAG TCCGGGGGGT
  241 GCGTGGCCCA GGACACCTCG GTGGTCCTTG ACTGGAACTA CCGCTGGATG CACGACGCAA
  301 ACTACAACTC GTGCACCGTC AACGGCGGCG TCAACACCAC GCTCTGCCCT GACGAGGCGA
  361 CCTGTGGCAA GAACTGCTTC ATCGAGGGCG TCGACTACGC CGCCTCGGGC GTCACGACCT
  421 CGGGCAGCAG CCTCACCATG AACCAGTACA TGCCCAGCAG CTCTGGCGGC TACAGCAGCG
  481 TCTCTCCTCG GCTGTATCTC CTGGACTCTG ACGGTGAGTA CGTGATGCTG AAGCTCAACG
  541 GCCAGGAGCT GAGCTTCGAC GTCGACCTCT CTGCTCTGCC GTGTGGAGAG AACGGCTCGC
  601 TCTACCTGTC TCAGATGGAC GAGAACGGGG GCGCCAACCA GTATAACACG GCCGGTGCCA
  661 ACTACGGGAG CGGCTACTGC GATGCTCAGT GCCCCGTCCA GACATGGAGG AACGGCACCC
  721 TCAACACTAG CCACCAGGGC TTCTGCTGCA ACGAGATGGA TATCCTGGAG GGCAACTCGA
  781 GGGCGAATGC CTTGACCCCT CACTCTTGCA CGGCCACGGC CTGCGACTCT GCCGGTTGCG
  841 GCTTCAACCC CTATGGCAGC GGCTACAAAA GCTACTACGG CCCCGGAGAT ACCGTTGACA
  901 CCTCCAAGAC CTTCACCATC ATCACCCAGT TCAACACGGA CAACGGCTCG CCCTCGGGCA
  961 ACCTTGTGAG CATCACCCGC AAGTACCAGC AAAACGGCGT CGACATCCCC AGCGCCCAGC
 1021 CCGGCGGCGA CACCATCTCG TCTGCTCGTC GTCTCAGCCT ACGGCGGCTC GCACATGGGC
 1081 AAGTCCTGAG CCAGCGCATG TGCTCGTGAT CAGCCAATTG GGAAACGGAA CAACAAGCCA
1141 GGTATAACAA TGA
//
Primer: f1ori_R3
Sequence file: RDB08308_A5Iib.seq check
>08308_08308_A5Ii_f1ori_R3_B01_04.ab1
    1 GATTGGCATC GGATTAGTTC CTCCTTTCAG CAAAACCCCT CAAGACCCGT TTAGAGGCCC
   61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGACATCC TCGGGGTCTT
  181 CCGGGGCGAG TTCTGGCTGG CTAGCCCGTT TGATCTCGAG TGCGGCCGCA AGCTTGTCGA
  241 CAAGGCATTG CGAGTAGTAG TCGTTGCTAT ACTGGCACGT AGTGCCCGAC GTGCACGTCT
  301 TGCACCCGCT GTACCCAATG CCACCGCACT GCCCCCAGTG AGTCTGCGTG CAGCTCGGGC
  361 TGCTCGAAGT CGTCGAGCTC CTCCGTGTAG TCGAAAACGT CGTGCTGGAC GCAGGCGGGG
  421 GCGGGGGCGC AGTCGAGTTC GTAGTAGACC CAATGTCTCC CCAGCGGATG TTGGAGAAGA
  481 CGACGTGCGT GTTGGGGTTG TTGGCCAGGA TGTTGGATGG GTTGCCCTCG GTGCTGCTGC
  541 AGGGGCCGGC GTTGCCGCTG TCGAGCCAGT TCATGTACTG GCTGTTGTCG TTCCAAATGC
  601 TGAACACGAG CACCATGCCG CTGCTCAGGG CCTTGCCCAT GGTGGCGAGG CCGCCGTAGG
  661 CTGAGGCGGA CGGGCAGGAC GAGATGGTGT CGCCGCCGGG CTGGGCGCTG GGGATGTCGA
  721 CGCCGTTTTG CTGGTACTTG CGGGTGATGC TCACAAGGTT GCCCGAGGGC GAGCCGTTGT
  781 CCGTGTTGAA CTGGGTGATG ATGGTGAAGG TCTTGGAGGT GTCAACGGTA TCTCCGGGGC
  841 CGTAGTAGCT TTTGTAGCCG CTGCCATAGG GGTTGAAGCC GCAACCGGCA GAGTCGCAGG
  901 CCGTGGCCGT GCAAGAGTGA GGGGTCAAGG CATTCGCCCT CGAGTTGCCC TCCAGGATAT
  961 CCATCTCGTT GCAGCAGAAG CCCTGGTGGG CTAGTGTTTG AGGGTGCCGT TCCTCCATGT
 1021 CTGGACGGGG CACTGGAGCA TCGCAGTAGC CGCCTCCCGT AGTGGCACCG GGCCGTGTTA
 1081 TACTGGGTGG GCGCCCCCGA TTCTCGGTTC CAATCTGAGA ACAGTTAGAG GCGGAGTCCG
1141 ATTCTTCTTC ATCAACGGGC CAGAAGCCAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles