Expression vector of Endoglucanase EG-II of Trichoderma reesei
Alternative name | pET25b_Tr004-His |
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Clone info. | Biomass collection. the Endoglucanase EG-II (EGLII, Endo-1,4-beta-glucanase, (EC 3.2.1.4)) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b. |
Vector backbone | pET-25b (Plasmid) |
Size of vector backbone | 5,547 bp |
Selectable markers | Amp^r |
Gene/insert name | Trichoderma reesei egl2 cDNA |
Depositor|Developer | DNA Bank, | Kishikawa, Shotaro | |
Sequence | RDB08309z.seq |
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Remarks, protocol and/or map (pdf) | RDB08309.pdf |
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Terms and conditions for distribution | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the RIKEN BRC is requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 理研BRCへの謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB08309 | pET25b_Tr-egl2-His | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pET25b_Tr-egl2-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08309). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17893_B0A6p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, RBS, pelB signal, insert5' Sequence file: RDB17893_B0A6a.seq ![]() |
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>08309_17893_B0A6_1_pET-B_F_A07_01_ABI24.ab1 1 NNNNNNNNNN NNGNNNNGCC GGCNNCGATG CGTCCGGCGT ANAGGNNCGA GATCTCGATC 61 CCGCGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCTCTAG 121 AAATAATTTT GTTTAACTTT AAGAAGGAGA TATACATATG AAATACCTGC TGCCGACCGC 181 TGCTGCTGGT CTGCTGCTCC TCGCTGCCCA GCCGGCGATG GCCATGGATA TCGGAATTAA 241 TTCGGATCCG AATTCGAGCT CCGTCGACCA GCAGACTGTC TGGGGCCAGT GTGGAGGTAT 301 TGGTTGGAGC GGACCTACGA ATTGTGCTCC TGGCTCAGCT TGTTCGACCC TCAATCCTTA 361 TTATGCGCAA TGTATTCCGG GAGCCACTAC TATCACCACT TCGACCCGGC CACCATCCGG 421 TCCAACCACC ACCACCAGGG CTACCTCAAC AAGCTCATCA ACTCCACCCA CGAGCTCTGG 481 GGTCCGATTT GCCGGCGTTA ACATCGCGGG TTTTGACTTT GGCTGTACCA CAGATGGCAC 541 TTGCGTTACC TCGAAGGTTT ATCCTCCGTT GAAGAACTTC ACCGGCTCAA ACAACTACCC 601 CGATGGCATC GGCCAGATGC AGCACTTCGT CAACGACGAC GGGATGACTA TTTTCCGCTT 661 ACCTGTCGGA TGGCAGTACC TCGTCAACAA CAATTTGGGC GGCAATCTTG ATTCCACGAG 721 CATTTCCNAG TATGATCAGC TTGTTCAGGN NTGCCTGTCT CTGGGCGCAT ACTGCATCGT 781 CGACATCCAC AATTATGCTC GATGGAACGG NGNNATCATT GGTCAGGGCG GCCCTACTAA 841 TGCTCNATTC ACGANCCTTN GNNCGCANTT GGCATCAAAG TACGCATCTC ANNCNANGGN 901 GTGGTTCNGN ATCNTGAATG ANCCCNNCGA NNNNNNNNTC NNNNCCNNGG NNNNNNCGNN 961 NNNNNANNNN NNNNNN // |
Primer: f1ori_R3 (Pr0142) Region: T7 ter, 6xHis, HSV tag, insert3' Sequence file: RDB17893_B0A6b.seq ![]() |
>08309_17893_B0A6_1_f1ori_R3_B07_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNCCTTTNN GCNNAAAACC CCTCNAGANC CGTTTAGAGG 61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC 121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGACA TCCTCGGGGT 181 CTTCCGGGGC GAGTTCTGGC TGGCTAGCCC GTTTGATCTC GAGTGCGGCC GCAAGCTTGT 241 CGACCTTTCT TGCGAGACAC GAGCTGACCA AGGATGTGTC CGTCCATGAG TTACCACTGC 301 CAGTCGGTGT TTCCGTCAGG ACATACGTGC TATCAAATGA TCCGGCACCC CAACCAACAT 361 AGCCAAGATA GACATCTGAG TTCTGGTTGA GATATTGGAT TTGCTGGCAC ATGTCTTGTA 421 TGCAGGACTG AACGTTGCCA CCACCGGTTT CTGTCAGGAT AGCCTGGCGA TTGTTCTGTC 481 GGAGCCAAGT GGCAAGCGGA GAAAAGGCGC CGTCAATGTT ATTTGTAGTA CATTCGGCGT 541 GAGTACCGGA GTTGTCTGAG TCCAAGTATT TGTGCACGTC AAAAATCAGA TTCGTTGTTG 601 ACCCATCCGG GTTCGTGACT TGAGACAGGG CGGCTGCACT GCCATCGGAT ATGAAAGCCC 661 CAGCAGATTG CCAATCATTT CNAGGCAAAG AGATGAATTG CGACGTAGCA CCAGCGTTGC 721 GGATTGCGGT TACAACCTCT TGGANCGTGG CAGCCCANGT GTTGATGTTC ACGTCGTNNN 781 GCTCATTCAT GATGCCGAAC CACACCCTCG ACTGANATGC GTACTTTGAT GNCAACTNCN 841 ANCAAANGNT CGNGANTTGA NCATTAGTAN GGCCGCCCNN ANCAATGATN CCACCGNTNC 901 NTCNANCNTA ATNNNNNNTG TCGANNANGC ANTATGCNNC CANNNNNAGG NNNNNNNNNA 961 NNNANNNNAN NNNNNNNNN // |
Primer: lacIpro_F2 (Pr0647) Region: lacI gene Sequence file: RDB17893_B0A6c.seq ![]() |
>08309_17893_B0A6_1_laclpro_F2_C07_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNG GNNNCGCATA AGGGNNANCG TCGAGNTCCC GGNNACCATC 61 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG 121 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT 181 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA 241 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG 301 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG 361 CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG 421 ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA 481 CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA 541 GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC 601 AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG 661 GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT 721 CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA 781 NGCGANTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC AAATGCTGAA TGANGGCATC 841 GTTCCNNCTG CNATGCTGGT TGCCNACGAT CAGATGGCGC NNGGCGCANT GCGCGCCATT 901 ANNNANNNCG GGNNGCNCGT TGGNNCNGNN NATNNCNNNA NNNGGNNACN ANNANNCCGN 961 ANNNNNNNNN N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
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