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pET25b_Tr-bgl1-His (#RDB08311)

Expression vector of beta-D-glucoside glucohydrolase 1 of Trichoderma reesei

Alternative name pET25b_Tr008-His
Clone info. Biomass collection. the beta-D-glucoside glucohydrolase 1 (bgl 1) gene, signal-peptide-less cds of Trichoderma reesei QM6a (syn. Hypocrea jecorina) is cloned in Sal I site on pET25b.
Vector backbone pET-25b (Plasmid)
Size of vector backbone 5,547 bp
Selectable markers Amp^r
Gene/insert name Trichoderma reesei bgl1 cDNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |
 
Sequence RDB08311z.seq
Remarks, protocol and/or map (pdf) RDB08311.pdf

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08311 pET25b_Tr-bgl1-His DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET25b_Tr-bgl1-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08311).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08311_A5Iip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB08311_A5Iia.seq check
>08311_08311_A5Ii_T7_20bp_E08_14.ab1
    1 TGCGTCGTTC CCTCTAGAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGAAAT
   61 ACCTGCTGCC GACCGCTGCT GCTGGTCTGC TGCTCCTCGC TGCCCAGCCG GCGATGGCCA
  121 TGGATATCGG AATTAATTCG GATCCGAATT CGAGCTCCGT CGACGACAGT AGCTCAACAT
  181 CGGGGGCCTC GGCTGAGGCA GTTGTACCTC CTGCAGGGAC TCCATGGGGA ACCGCGTACG
  241 ACAAGGCGAA GGCCGCATTG GCAAAGCTCA ATCTCCAAGA TAAGGTCGGC ATCGTGAGCG
  301 GTGTCGGCTG GAACGGCGGT CCTTGCGTTG GAAACACATC TCCGGCCTCC AAGATCAGCT
  361 ATCCATCGCT ATGCCTTCAA GACGGACCCC TCGGTGTTCG ATACTCGACA GGCAGCACAG
  421 CCTTTACGCC GGGCGTTCAA GCGGCCTCGA CGTGGGATGT CAATTTGATC CGCGAACGTG
  481 GACAGTTCAT CGGTGAGGAG GTGAAGGCCT CGGGGATTCA TGTCATACTT GGTCCTGTGG
  541 CTGGGCCGCT GGGAAAGACT CCGCAGGGCG GTCGCAACTG GGAGGGCTTC GGTGTCGATC
  601 CATATCTCAC GGGCATTGCC ATGGGTCAAA CCATCAACGG CATCCAGTCG GTAGGCGTGC
  661 AGGCGACAGC GAAGCACTAT ATCCTCAACG AGCAGGAGCT CAATCGAGAA ACCATTTCGA
  721 GCAACCCAGA TGACCGAACT CTCCATGAGC TGTATACTTG GCCATTTGCC GACGCGGTTC
  781 AGGCCAATGT CGCTTCTGTC ATGTGCTCGT ACAACAAGGT CAATACCACC TGGGCCTGCG
  841 AGGATCAGTA CACGCTGCAG ACTGTGCTGA AAGACCAGCT GGGGTTCCCA GGCTATGTCA
  901 TGACGGACTG GAACGCACAG CACACGACTG TCCAAAGCGC GAATTCTGGG CTTGACATGT
  961 CAATGCCTGG CACAGACTTC AACGGTAACA ATCGGCTCTG GGGGTCCAGC TCTCACCCAA
 1021 TGCGGGTAAA TAGCAAATCA TGTCCCCCAC GAGCAGAGTC GACGGATATG GTGACTCGTA
 1081 TCCTCGCCGC AATGTACTGA CAGCCAGGAC CAGGCCAGGC TATATCCCGG TTCGGTTTCC
//
Primer: f1ori_R3
Sequence file: RDB08311_A5Iib.seq check
>08311_08311_A5Ii_f1ori_R3_F08_17.ab1
    1 GCATCCATCG GATTAGTTCC TCCTTTCAGC AAAAACCCCT CAAGACCCGT TTAGAGGCCC
   61 CAAGGGGTTA TGCTAGTTAT TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG
  121 CTTTGTTAGC AGCCGGATCT CAGTGGTGGT GGTGGTGGTG CTCGACATCC TCGGGGTCTT
  181 CCGGGGCGAG TTCTGGCTGG CTAGCCCGTT TGATCTCGAG TGCGGCCGCA AGCTTGTCGA
  241 CCGCTACCGA CAGAGTGCTC GTCAGCCTGA TATCCCGGCT GCTCGCTCCC ACGCTGATGC
  301 CAAACGACCC CGACGGCACC ACCCATTTCT GCGAAGCCGT GTCCCAGTAG CTGAGATCTC
  361 GTCGTCGGAT GTTGAACGTT GCTGTTCCGC TCTGACCAGG CGTGAGGTTC AGCTTGGCAA
  421 AGCCTCGCAG CTGCTTCGGA GGGGTCCTGG GTGCTGAAGA TGGGTAGGTG ATGTACAGCT
  481 GGGCTACCTC GGCACCAGTC ACTTGGCCAG AGTTTGCGAT GTCAACGGTG ACTGTCGCGA
  541 CATTCTGGAA CAGATCACTC GGGCCTCCCG GCACAACGGC CCCAGTCGCA GGACCAGACT
  601 TGGCGGTCGA CAAGACGGAG AGGCGTGAGT AGTTGAACTT GGTGTAAGAC AGTCCATAGC
  661 CGAACTCGTA CCGCGGCGTG ATATTGGCGT CGTCGAAGTG CTTATAGTCG ATGAACAGTC
  721 CCTCGCTGAA GCTGTCACTG CCGCCGGAAA CGATGCGAGT GTTATAGTCA TTGGGGCTCT
  781 TCGCAATGGT GTACACCAGC TTGCCAGAAG GGCTGACATC TCCCCACAGC ACGTCGACGA
  841 GCGCATTGCC GCTCTCCTGA GAAGGAAGAC CCGCCCAGAC AACGGCCTTG ACCTGCGGAA
  901 GAGCAAGAAT CTGCTCCAGA ATGATGGCGC CAACGGAGTG GACAACAACA ATGACGTTGC
  961 TGTTGGCACC GGCCACCGCC TGGACCAGGG CATTGCCGTT GTGCCACGGG ATCCAGGGTT
 1021 GTTGCGATCG CCCGCGTTTG CCCTCCACGG TGATGTAGCC CTTTCACCCC GAGTCGGCGT
 1081 GATGAGACGA TGGGCGAACG TCCTTCCTCT TGCCTGCAGG ATGCGCCTGA GGGACGTGTT
1141 TGATCAGGGT GGATC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles