Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-UbC-RfA-IRES2-hKO1

Backbone vector plasmid of lentivirus construct driven by UbC promoter by Gateway(R) cloning with hKO1 fluorescent protein marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14535_A7Ajp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro_F (Pr0158) Region: UbC pro,attR1,CmR Sequence file: RDB14535_A7Aja.seq
>08350_14535_A7Aj_1_UbCpro_F_A07_01_ABI24.ab1     1 AGCTTGTATT TTCAGTGTTA GACTAGTAAA TTGTCCGCTA AATTCTGGCC GTTTTTGGCT    61 TTTTTGTTAG ACCGGACCGT GTTTAGCGCT ACCGGTCTCG AGAATTCTGC AGCGGCCATC   121 ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT CAATATATTA   181 AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA TATCCAGTCA   241 CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATAATG   301 TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT AAAATGGAGA   361 AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA GAACATTTTG   421 AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG GATATTACGG   481 CCTTTTTAAA GACCGTAAAG AAAAATAAGC ACAAGTTTTA TCCGGCCTTT ATTCACATTC   541 TTGCCCGCCT GATGAATGCT CATCCGGAAT TCCGTATGGC AATGAAAGAC GGTGAGCTGG   601 TGATATGGGA TAGTGTTCAC CCTTGTTACA CCGTTTTCCA TGAGCAAACT GAAACGTTTT   661 CATCGCTCTG GAGTGAATAC CACGACGATT TCCGGCAGTT TCTACACATA TATTCGCAAG   721 ATGTGGCGTG TTACGGTGAA AACCTGGCCT ATTTCCCTAA AGGGTTTATT GAGAATATGT   781 TTTTCGTCTC AGCCAATCCC TGGGTGAGTT TCACCAGTTT TGATTTAAAC GTGGCCAATA   841 TGGACAACTT CTTCGCCCCC GTTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG   901 GTGCTGATGC CGCTGGCGAT TCAGTTCATC ATGCCGTTTG TGATGGCTTC CATGTCGGCA   961 GAATGCTTAA TGAATTACAA CAGTACTGCG ATGAGTGGGC AGGCGGGGCG TAAACGCGTG  1021 GATCCCGGCT TACTAAAAAG CCAGATACAG TATGCGTATT TTGCGCGCTG ATTTTTTGCG  1081 GTATAAGATA TATACTGATA TGTATACCGG AAGTATGGTC AAAAAGGAGG GTAATGCCTA //
Primer: IRES-R (Pr0037) Region: IRES2,attR2,ccdB Sequence file: RDB14535_A7Ajb.seq
>08350_14535_A7Aj_IRES-R_G10_07_ABI08.ab1     1 GGCATCGCGG CCTTCGGCAG TACGTTAGGG GGGGGGGAGG GAGAGGGGCG GATCCGCGGC    61 CATCACCACT TTGTACAAGA AAGCTGAACG AGAAACGTAA AATGATATAA ATATCAATAT   121 ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC AACATATCCA   181 GTCACTATGG TCGACCTGCA GACTGGCTGT GTATAAGGGA GCCTGACATT TATATTCCCC   241 AGAACATCAG GTTAATGGCG TTTTTGATGT CATTTTCGCG GTGGCTGAGA TCAGCCACTT   301 CTTCCCCGAT AACGGAGACC GGCACACTGG CCATATCGGT GGTCATCATG CGCCAGCTTT   361 CATCCCCGAT ATGCACCACC GGGTAAAGTT CACGGGAGAC TTTATCTGAC AGCAGACGTG   421 CACTGGCCAG GGGGATCACC ATCCGTCGCC CGGGCGTGTC AATAATATCA CTCTGTACAT   481 CCACAAACAG ACGATAACGG CTCTCTCTTT TATAGGTGTA AACCTTAAAC TGCATTTCAC   541 CAGCCCCTGT TCTCGTCAGC AAAAGAGCCG TTCATTTCAA TAAACCGGGC GACCTCAGCC   601 ATCCCTTCCT GATTTTCCGC TTTCCAGCGT TCGGCACGCA GACGACGGGC TTCATTCTGC   661 ATGGTTGTGC TTACCAGACC GGAGATATTG ACATCATATA TGCCTTGAGC AACTGATAGC   721 TGTCGCTGTC AACTGTCACT GTAATACGCT GCTTCATAGC ATACCTCTTT TTGACATACT   781 TCGGGTATAC ATATCAGTAT ATATTCTTAT ACCGCAAAAA TCAGCGCGCA AATACGCATA   841 CTGTTATCTG GGCTTTTAGT AAGCCGGATC CACGCGTTTA CGCCAGCCGC CCCTGCCACT   901 CATCGCAGTA CTGTTTGTAA ATTCATTTAA AGCATTTCTG CCCGACATGG GAAAGCCCAA   961 TCACAAACGG GCCATGGATT GAAACCTGGA AATCGCCAGC GGGCATCAGC ACCCTTTGGT  1021 CCGCCCTTGC GTATAAATAT TTTGGCCCCA ATGGATGGAA AAAACGGGGG GGGGCCGGAA 1081 AGGAAAGGAT GC //
Primer: IRES-F (Pr0036) Region: IRES2,hKO,PRE Sequence file: RDB14535_A7Ajc.seq
>08350_14535_A7Aj_1_IRES-F_G07_19_ABI24.ab1     1 GCGAAGCCTT TAAAATGGTG TTTAGTCGGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC    61 ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACGGG GATCCCCACC   121 ATGGTGAGCG TGATCAAGCC CGAGATGAAG ATGAAGTACT TCATGGACGG CAGCGTGAAC   181 GGCCACGAGT TCACCGTGGA GGGCGAGGGC ACCGGCAAGC CCTACGAGGG CCACCAGGAG   241 ATGACCCTGA GGGTGACAAT GGCCAAGGGC GGCCCCATGC CCTTCAGCTT CGACCTGGTG   301 AGCCACACCT TCTGCTACGG CCACAGGCCC TTCACCAAGT ACCCCGAGGA GATCCCCGAC   361 TACTTCAAGC AGGCCTTCCC CGAGGGCCTG AGCTGGGAGA GGAGCCTCCA GTTCGAGGAC   421 GGCGGCTTCG CCGCCGTGAG CGCCCACATC AGCCTGAGGG GCAACTGCTT CGAGCACAAG   481 AGCAAGTTCG TGGGCGTGAA CTTCCCCGCC GACGGCCCCG TGATGCAGAA CCAGAGCAGC   541 GACTGGGAGC CCAGCACCGA GAAGATCACC ACCTGCGACG GCGTGCTGAA GGGCGACGTG   601 ACCATGTACC TGAAGCTGGC CGGCGGCGGC AACCACAAGT GCCAGTTCAA GACCACCTAC   661 AAGGCCGCCA AGAAGATCCT GAAGATGCCC CAGAGCCACT TCATCGGCCA CAGGCTGGTG   721 AGGAAGACCG AGGGCAACAT CACCGAGCTG GTGGAGGACG CCGTGGCCCA CTGCTGACTG   781 CAGGCTAGAG TTAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA   841 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG   901 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTTCC   961 TTGTAATAAA TTCCTGGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGG TTGTCAGGCA  1021 ACGGTGGCCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTT GGGGGCATTG  1081 GCACACTGTC AGGCTCCCTT TCCGGACTTC GCTTTTCCCC CGTCCTAATT GTGGCCACCG 1141 GGCCGAACCA TCCATTTCG //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1psi Sequence file: RDB14535_A7Ajd.seq
>08350_14535_A7Aj_1_CMV-Forward_B08_05_ABI24.ab1     1 AGTCGCTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT   121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG   181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT   241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC   301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG   361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG   421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT   481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT   541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT   601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC   661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT   721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA   781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGGA GAAGTGAATT ATATAAATAT   841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AAGGCAAAGA GAAGAGTGGT   901 GCAGAGAGAA AAAAGAGCAG TTGGAATAGG AGCTTTTGTT CCTTGGGTTC TTGGGGAGCA   961 GCAAGAGCAC TATGGGGCGC AGCGTTCATT GACGCTGACG GTACAGTAGA CATAATGTCT  1021 GCTATAGTGC AGCAGCGACC ACTTGCCTGA CGCTATGAGG CCACAGCATC TGTGCCACCT 1081 CACAAG //
Primer: f1ori_R3 (Pr0142) Region: bGHpA,3' LTR Sequence file: RDB14535_A7Aje.seq
>08350_14535_A7Aj_1_f1ori_R3_E08_14_ABI24.ab1     1 CGATGGACTG AGCCAGCTGG TTCTTTCCGC CTCAGAAGCC ATAGAGCCCA CCGCATCCCC    61 AGCATGCCTG CTATTGTCTT CCCAATCCTC CCCCTTGCTG TCCTGCCCCA CCCCACCCCC   121 CAGAATAGAA TGACACCTAC TCAGACAATG CGATGCAATT TCCTCATTTT ATTAGGAAAG   181 GACAGTGGGA GTGGCACCTT CCAGGGTCAA GGAAGGCACG GGGGAGGGGC AAACAACAGA   241 TGGCTGGCAA CTAGAAGGCA CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG   301 AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA   361 GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT   421 TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC   481 AGTCCGGATG CAGCTCTCGG GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT   541 CTAATACTTC TCTCTCCGGG TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG   601 TGTTCTCTCC TTCATTGGCT TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC   661 ACCATCCAAA GGTCAGTGGA TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG   721 GGAAGTAGCC TTGTGTGTGG TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT   781 GAATTAGCCC TTTCCAGTCC CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC   841 TTGTAAGTCA TTGGTCTTAA GGTACCTGAG TGTGACTGGA AAACCCACCT CTCTCCTCTG   901 TGCTTCTAGC CAGGCACAAT CAGCATGGTA GCTGCTGTAT TGCTACTGTG ATGGCTCATG   961 TTTTTTCTAG TCTCGACGGT ATCGAATGCC GGAGCCGCTA AACGGGAATT CCGAACTCGA 1021 TCTGAAGACT AGTAGACCGA AGCTCAGCGT CACAGTCCGG AGGAAGTC //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori,Zeo,SV40pA Sequence file: RDB14535_A7Ajf.seq
>08350_14535_A7Aj_1_SV40pro_F_H08_23_ABI24.ab1     1 CCTGTCCTTG CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA    61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA   121 TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG   181 TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG   241 ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG   301 GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG   361 GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC   421 ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG   481 CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG   541 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC   601 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG   661 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC   721 ATCACAAATT TCACAAATAA AGCATTTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA   781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA   841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT   901 ACGAGCCGGA AGCATAAAGT GTAAAGCCCT GGGGGTGCCT AATGAGTGAG CTAACTCACA   961 TTAAATTGCG TTTGCGCTCA CTGCCCGCTT TCCAGTCGGA AACCTGTCGT GCCAGCTGCA  1021 TTAAATGAAA TCGGGCACGC GCGGGAGGAG GCGGGTTGCG TATGGGGCGC TCTTCGCTTC  1081 CTCGATCCAC TTGAACTCGC TGCGCCTCGG ATCGGATTCC GGCCTTGCCG GGGAGAGCCG 1141 GGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.16

GNP_filter3_RDBDEP_html_231027.pl