RDB08404 - pET-G8(M)-1a

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pET-G8(M)-1a (#RDB08404)

Bacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M), tag-free protein

Clone info.	Bacterial expression clone of human galectin-8.
Comment	Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone	pET-11a (Plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	Human galectin-8 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Sequence	RDB08404z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、学術機関の学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08404	pET-G8(M)-1a	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-G8(M)-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08404).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17484_A9Hsp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7pro, lacO, insert5' Sequence file: RDB17484_A9Hsa.seq
>08404_17484_A9Hs_2_pET-B_F_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NNNGCCGGNN NCGNTGCGTC CGGCGTAGAG GNNCGAGATC 61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC 121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGATGT TGTCCTTAAA 181 CAACCTACAG AATATCATCT ATAACCCGGT AATCCCGTTT GTTGGCACCA TTCCTGATCA 241 GCTGGATCCT GGAACTTTGA TTGTGATACG TGGGCATGTT CCTAGTGACG CAGACAGATT 301 CCAGGTGGAT CTGCAGAATG GCAGCAGCAT GAAACCTCGA GCCGATGTGG CCTTTCATTT 361 CAATCCTCGT TTCAAAAGGG CCGGCTGCAT TGTTTGCAAT ACTTTGATAA ATGAAAAATG 421 GGGACGGGAA GAGATCACCT ATGACACGCC TTTCAAAAGA GAAAAGTCTT TTGAGATCGT 481 GATTATGGTG CTGAAGGACA AATTCCAGGT GGCTGTAAAT GGAAAACATA CTCTGCTCTA 541 TGGCCACAGG ATCGGCCCAG AGAAAATAGA CACTCTGGGC ATTTATGGCA AAGTGAATAT 601 TCACTCAATT GGTTTTAGCT TCAGCTCGGA CTTACAAAGT ACCCAAGCAT CTAGTCTGGA 661 ACTGACAGAG ATAAGTAGAG AAAATGTTCC AAAGTCTGGC ACGCCCCAGC TTAGGCTGCC 721 ATTCGCTGCA AGGTTGAACA CCCCCATGGG CCCTGGACGA ACTGTCGTCG TTAAAGGAGA 781 AGTGAATGCA AATGCCAAAA GCTTTAATGT TGANCTACTA GCANGAAAAT CAAAGGANAT 841 TGCTCTACAC TTGAACNCAC GCCTGAATAT TNAAGCATTT G //
Primer: pET-check-R1 (Pr0192) Region: T7 ter, insert3' Sequence file: RDB17484_A9Hsb.seq
>08404_17484_A9Hs_2_pET-check_R1_E08_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CAGTCAGGCA CCGTGTATGA AATCTAACAA 61 TGCGCTCATC GTCATCCTCG GCACCGTCAC CCTGGATGCT GTAGGCATAG GCTTGGTTAT 121 GCCGGTACTG CCGGGCCTCT TGCGGGATAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA 181 CCCCTCAAGA CCCGTTTAGA GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA 241 GCAGCCAACT CAGCTTCCTT TCGGGCTTTG TTAGCAGCCG GATCTCTACC AGCTCCTTAC 301 TTCCAGTAAG TGGATGTCTC CATTAATTTC CAGCGTGTCA ATACTGCTGA GCTCTTTAAA 361 TCTGTGTTTG TACTCCAGGC TGTGTACGCC ATTTACTGCA ACCTTGAATT CTCTAACATC 421 ACAATAAATT ATCATCTCAA AGTACATCCC AGGACTAAAT GGGAAAGAGG TAATATTTCT 481 CTCTTCTTCT CCCCAGGACT CCTGAAGAAA AGAATTTCTT ACAAATGCTT TAATATTCAG 541 GCGTGGGTTC AAGTGTAGAG CAATATCCTT TGATTTTCCT GCTAGTAGGT CAACATTAAA 601 GCTTTTGGCA TTTGCATTCA CTTCTCCTTT AACGACGACA GTTCGTCCAG GGCCCATGGG 661 GGTGTTCAAC CTTGCAGCGA ATGGCAGCCT AAGCTGGGGC GTGCCAGACT TTGGAACATT 721 TTCTCTACTT ATCTCTGTCA GTTCCAGACT AGATGCTTGG GTACTTTGTA AGTCCGAGCT 781 GAAGCTAAAA CCAATTGAGT GAATATTCAC TTTGCCATAA ATGCCCAGAG TGTCTATTTT 841 CTCTGGNNCG ATCCTGTGGC CATANAGCAN ANTATGTTTT CCNNTTACAG CCACCTGNAA 901 NTTGTCCTTC //
Primer: lacIpro_F2 (Pr0647) Region: lacIpro, lacI Sequence file: RDB17484_A9Hsc.seq
>08404_17484_A9Hs_2_lacIpro_F2_F08_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNGCNG GNNTCGCATA AGGGAGAGCG TCGAGNTCCC GGACACCATC 61 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG 121 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT 181 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA 241 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG 301 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG 361 CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG 421 ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA 481 CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA 541 GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC 601 AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG 661 GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT 721 CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA 781 NGCGACTGGA GTGCCATGTC CGGTTTTCAA CNAACCATGC AAATGCTGAA TGANGGCATC 841 GTTCCCACTG CGATGCTGGT TGNCNACGAT CAGATGGNGC TGGGNGCAAT GCGCGCCATT 901 NCCGAGTCCG GGCTGCGCGT TGGTGCGGAN NTCTCGGTAN TGGGATANNA CNATACCGAA 961 GANAGCTCAN GTTATATCCC GCCGTT //