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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET-G9(M)-4a (#RDB08420)

Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M), tag-free protein

Clone info. Bacterial expression clone of human galectin-9.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pET-11a (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Human galectin-9 cDNA
Depositor|Developer Nishi, Nozomu |
 
Sequence RDB08420z.seq

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08420 pET-G9(M)-4a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-G9(M)-4a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08420).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17483_A9Hsp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723)
Region: T7pro, lacO, insert5'
Sequence file: RDB17483_A9Hsa.seq check
>08420_17483_A9Hs_3_pET-B_F_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNGCCGGC NNCNATGCGT CCGGCGTAGA GGNNCGAGAT
   61 CTCGATCCCG CGNAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC
  121 CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGGCC TTCAGCGGTT
  181 CCCAGGCTCC CTACCTGAGT CCAGCTGTCC CCTTTTCTGG GACTATTCAA GGAGGTCTCC
  241 AGGACGGACT TCAGATCACT GTCAATGGGA CCGTTCTCAG CTCCAGTGGA ACCAGGTTTG
  301 CTGTGAACTT TCAGACTGGC TTCAGTGGAA ATGACATTGC CTTCCACTTC AACCCTCGGT
  361 TTGAAGATGG AGGGTACGTG GTGTGCAACA CGAGGCAGAA CGGAAGCTGG GGGCCCGAGG
  421 AGAGGAAGAC ACACATGCCT TTCCAGAAGG GGATGCCCTT TGACCTCTGC TTCCTGGTGC
  481 AGAGCTCAGA TTTCAAGGTG ATGGTGAACG GGATCCTCTT CGTGCAGTAC TTCCACCGCG
  541 TGCCCTTCCA CCGTGTGGAC ACCATCTCCG TCAATGGCTC TGTGCAGCTG TCCTACATCA
  601 GCTTCCAGCC TCCCGGCGTG TGGCCTGCCN ACCCGGCTCC CATTACCCAN ACNGTCATCC
661 NCNNNGTGCA GAGCGCCCCT GGACAGATGT TCTCTACTCC CGCCATCCCC ACNN
//
Primer: tetpro-B_R (Pr0732)
Region: T7 ter, insert3'
Sequence file: RDB17483_A9Hsb.seq check
>08420_17483_A9Hs_3_tetpro-B_R_C03_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNANNG CGGTAGTTTA TCACAGTTAA
   61 ATTGCTNNAC GCAGTCAGGC ACCGTGTATG AAATCTAACA ATGCGCTCAT CGTCATCCTC
  121 GGCACCGTCA CCCTGGATGC TGTAGGCATA GGCTTGGTTA TGCCGGTACT GCCGGGCCTC
  181 TTGCGGGATA TCCGGATATA GTTCCTCCTT TCAGCAAAAA ACCCCTCAAG ACCCGTTTAG
  241 AGGCCCCAAG GGGTTATGCT AGTTATTGCT CAGCGGTGGC AGCAGCCAAC TCAGCTTCCT
  301 TTCGGGCTTT GTTAGCAGCC GGATCTCTAT GTCTGCACAT GGGTCAGCTG GATGTCGCCC
  361 CCCACTTCCA GTCTGTTGAT GGTGGGCAGG TTCCTCAGGC GATGGTAGTA TTCAAACAGG
  421 TGCTGACCAT CCACGGCCAC CTTGAGGCAG TGAGCTTCAC ACAAGATCCA CACTGAGAAG
  481 CTCTGGCCAC GGACGAAGGG CATTTTTCGG GGCAGACTTC GCTCCTCAGA CCCCCAGGAG
  541 TTGTCGATCT GGGTGTTGCG GACCACAGCA TTCTCATCAA AACGGGGGTT CAGGTGGAAG
  601 GCGATGTGGT TCCCAGAGCA CAGGTTGATG TGGAACCTCT GANCACTGGG CAGGACAGTG
  661 CCTGACAGGA GGATGGACTT GGATGGGTAC AGCCCTCCCA GAATGGTGGT GATGAAAGGC
721 ATCGGATAGG CGGGGTGGGG GTACATCATA NG
//
Primer: lacIpro_F2 (Pr0647)
Region: lacI pro, lacI
Sequence file: RDB17483_A9Hsc.seq check
>08420_17483_A9Hs_3_lacIpro_F2_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN GNNTCGCATA AGGGAGAGCG TCGAGATCCC GGACACCATC
   61 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG
  121 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT
  181 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA
  241 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG
  301 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG
  361 CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG
  421 ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA
  481 CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA
  541 GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC
  601 AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG
  661 GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT
  721 CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA
  781 GGCGACTGGA GTGCCATGTC CGGTTTTCNA CNAACCATGC NNNTGCTGAA TGAGGGCATC
  841 GTTCCCACTG CGATGCTGGT TGCCNACGAT CAGATGGNGC TNGGCGCAAT GCGCGCCNTT
  901 ACCGAGNNCG GGCTGCGCGT TGGTGCGGAN ATCTCGGNAG NNGGATANGA CGATANNNAN
961 GNNAGCTCAT GTNATATCCC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]