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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-G12-1a (#RDB08428)

Bacteria expression vector of human galectin-12, GST fusion protein

Clone info. Bacterial expression clone of human galectin-12 (with a shorter N-terminal sequence).
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-12 cDNA
Depositor|Developer Nishi, Nozomu |
 
Sequence RDB08428z.seq

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08428 pGEX-G12-1a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-G12-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08428).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08428_A8Bsp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB08428_A8Bsa.seq check
>08428_08428_A8Bs_pGEX-F2_A01_01_ABI08.ab1
    1 CTTTTTAGTT TTGGTTTTAA AAACGTATTG AAGCTATCCC ACAAAATTGA TAAGTACTTG
   61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC
  121 GACCATCCTC CAAAATCGGA TCTGGTTCCG CGTGGATCCA TGTCACCTGG AGAAAAACTG
  181 GACCCAATTC CTGACAGCTT CATTCTGCAA CCACCAGTCT TCCACCCGGT GGTTCCTTAT
  241 GTCACGACGA TTTTTGGAGG CCTGCATGCA GGCAAGATGG TCATGCTGCA AGGAGTGGTC
  301 CCTCTAGATG CACACAGGTT TCAGGTGGAC TTCCAGTGTG GCTGCAGCCT GTGTCCCCGG
  361 CCAGATATCG CCTTCCACTT CAACCCTCGC TTCCATACCA CCAAGCCCCA TGTCATCTGC
  421 AACACCCTGC ATGGTGGACG CTGGCAAAGG GAGGCCCGGT GGCCCCACCT GGCCCTGCGA
  481 AGAGGCTCCA GCTTCCTCAT CCTCTTTCTC TTCGGGAATG AGGAAGTGAA GGTGAGTGTG
  541 AATGGACAGC ACTTTCTCCA CTTCCGCTAC CGGCTCCCAC TGTCTCATGT GGACACGCTG
  601 GGTATATTTG GTGACATCCT GGTAGAGGCT GTTGGATTCC TGAACATCAA TCCATTTGTG
  661 GAGGGCAGCA GAGAGTACCC AGCTGGACAT CCTTTCCTGC TGATGAGCCC CAGGCTGGAG
  721 GTGCCCTGCT CACATGCTCT TCCCCAGGGT CTCTCGCCTG GGCAGGTCAT CATAGTACGG
  781 GGACTGGTCT TGCAAGAGCC GAAGCATTTT ACTGTGAGCC TGAGGGACCA GGCTGCCCAT
  841 GCTCCTGTGA CACTCAGGGC CTCCTTCGCA GACAGAACTC TGGCCTGGAT CTCCCGCTGG
  901 GGGCAGAAGA AACTGATCTC AGCCCCCTTC CTCTTTTACC CCCAGAGATT CTTTGAGGTG
  961 CTGCTCCTGT TCCAGGAGGG AGGGCTGAAG CTGGCGCTCA ATGGGCAGGG GCTGGGGGGC
 1021 CACCAGCATG GAACCAGCAG GCCCTGCAGC AGCTGCGGGG AGCTCGGGAT CAGTGGAAGT
 1081 GTCCAGCCTC TACTGTGTCC ACCTCCTGAG ATTTCCCGGG ATCGAACTCG AGCGGCCGCA
1141 TCCGTGAACT GACTGAACGA TCCTGGCCTT CGGCCGCGCG GTTTTCGCGA TA
//
Primer: pGEX-RSEQ
Sequence file: RDB08428_A8Bsb.seq check
>08428_08428_A8Bs_pGEX-RSEQ_B01_02_ABI08.ab1
    1 GTTTATTCCG GAAAAAGCGC CGAAGGCAAG ATCGTCAGTC AGTCACGATG CGGCCGCTCG
   61 AGTCGACCCG GGAATTCTCA GGAGTGGACA CAGTAGAGCT GGACACTTCC ACTGATCCGG
  121 AGCTCCCGCA GCTGCTCCAG GGCCTGCTGG TTCATGCTGG TGGCCCCCAG CCCCTGCCCA
  181 TTGAGCGCCA GCTTCAGCCC TCCCTCCTGG AACAGGAGCA GCACCTCAAA GAATCTCTGG
  241 GGGTAAAAGA GGAAGGGGGC TGAGATCAGT TTCTTCTGCC CCCAGCGGGA GATCCAGGCC
  301 AGAGTTCTGT CTGCGAAGGA GGCCCTGAGT GTCACAGGAG CATGGGCAGC CTGGTCCCTC
  361 AGGCTCACAG TAAAATGCTT CGGCTCTTGC AAGACCAGTC CCCGTACTAT GATGACCTGC
  421 CCAGGCGAGA GACCCTGGGG AAGAGCATGT GAGCAGGGCA CCTCCAGCCT GGGGCTCATC
  481 AGCAGGAAAG GATGTCCAGC TGGGTACTCT CTGCTGCCCT CCACAAATGG ATTGATGTTC
  541 AGGAATCCAA CAGCCTCTAC CAGGATGTCA CCAAATATAC CCAGCGTGTC CACATGAGAC
  601 AGTGGGAGCC GGTAGCGGAA GTGGAGAAAG TGCTGTCCAT TCACACTCAC CTTCACTTCC
  661 TCATTCCCGA AGAGAAAGAG GATGAGGAAG CTGGAGCCTC TTCGCAGGGC CAGGTGGGGC
  721 CACCGGGCCT CCCTTTGCCA GCGTCCACCA TGCAGGGTGT TGCAGATGAC ATGGGGCTTG
  781 GTGGTATGGA AGCGAGGGTT GAAGTGGAAG GCGATATCTG GCCGGGGACA CAGGCTGCAG
  841 CCACACTGGA AGTCCACCTG AAACCTGTGT GCATCTAGAG GGGACCACTC CTTGCAGCAT
  901 GACCATCTTG CCTGCATGCA GGCCTCCAAA AATCGTCGTG ACATAAGGAA CCACCGGGTG
  961 GAAGACTGGT GGTTGCAGAA TGAAGCTGTC AGGAATTGGG TCCAGTTTTT CTCCAGGTGA
 1021 CATGGATCCA CGCCGGACCA GATCCGAATT TTGGAAGGAT GGGTCGCCAC CACCAACGTG
 1081 ACTTGCCAGC CTGCAAGGCA TGCTATATAC TTGCTGGATT CAGGTACTAT CAATTGTGGA
1141 ATAGGCCTTT CATAACGTTT TTTTAGAAAC AAAACCTATA T
//
Primer: M13_-40
Sequence file: RDB08428_A2Cmc.seq check
>08428_08428_A2Cm_M13_-40_C01_07_ABI24.ab1
    1 AGGCAAGTGA TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
   61 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
  121 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
  181 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
  241 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT
  301 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT
  361 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA
  421 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA
  481 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT
  541 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT
  601 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG
  661 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA
  721 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG
  781 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT
  841 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA GGATTGTGCA
  901 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACA ACGCTGGCAC
  961 CCAGGTTGAT CGGCGCGAGA TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGCAGA
 1021 CTGAGTGGCA ACGGCCAATC AGCAACGACT GTTGGCCGCA GTTGTTTGTG CAGCGAGGGA
1081 TGTAATCAGG TCCGCATGCC GCTTCCACTT TTCGGTTCGG AA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]