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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-G12NT-1b (#RDB08429)

Bacteria expression vector of human galectin-12 (N-terminal CRD with a part of the linker peptide, G12NT), GST fusion protein

Clone info. Bacterial expression clone of an N-terminal CRD of human galectin-12 (with a shorter N-terminal sequence) with a part of linker peptide.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-12 cDNA
Depositor|Developer Nishi, Nozomu |
 
Sequence RDB08429z.seq

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08429 pGEX-G12NT-1b DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-G12NT-1b was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08429).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08429_A8Bsp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB08429_A8Bsa.seq check
>08429_08429_A8Bs_pGEX-F2_C01_03_ABI08.ab1
    1 GATAAGGTTT TGGTTTTTAA AAAACGTTAT TGAAGCTATC CCACAAATTG ATAAGTACTT
   61 GAAATCCAGC AAGTATATAG CATGGCCTTT GCAGGGCTGG CAAGCCACGT TTGGTGGTGG
  121 CGACCATCCT CCAAAATCGG ATCTGGTTCC GCGTGGATCC ATGTCACCTG GAGAAAAACT
  181 GGACCCAATT CCTGACAGCT TCATTCTGCA ACCACCAGTC TTCCACCCGG TGGTTCCTTA
  241 TGTCACGACG ATTTTTGGAG GCCTGCATGC AGGCAAGATG GTCATGCTGC AAGGAGTGGT
  301 CCCTCTAGAT GCACACAGGT TTCAGGTGGA CTTCCAGTGT GGCTGCAGCC TGTGTCCCCG
  361 GCCAGATATC GCCTTCCACT TCAACCCTCG CTTCCATACC ACCAAGCCCC ATGTCATCTG
  421 CAACACCCTG CATGGTGGAC GCTGGCAAAG GGAGGCCCGG TGGCCCCACC TGGCCCTGCG
  481 AAGAGGCTCC AGCTTCCTCA TCCTCTTTCT CTTCGGGAAT GAGGAAGTGA AGGTGAGTGT
  541 GAATGGACAG CACTTTCTCC ACTTCCGCTA CCGGCTCCCA CTGTCTCATG TGGACACGCT
  601 GGGTATATTT GGTGACATCC TGGTAGAGGC TGTTGGATTC CTGAACATCA ATCCATTTGT
  661 GGAGGGCAGC TGAGAATTCC CGGGTCGACT CGAGCGGCCG CATCGTGACT GACTGACGAT
  721 CTGCTCGCGC GTTTCGGTGA TGACGGTGAA AACTCTGACC ATGCAGCTCC CGAGAACGTT
781 CCACAGCCTT GTTCTGTAAG CCGGAATGGC CG
//
Primer: M13_-40
Sequence file: RDB08429_A2Cmb.seq check
>08429_08429_A2Cm_M13_-40_F01_16_ABI24.ab1
    1 AGGGCCAATC CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
   61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
  121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
  181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
  241 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
  301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
  361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
  421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
  481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
  541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
  601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
  661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
  721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
  781 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
  841 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC
  901 CGCCGCTTTA CAGGCTTCGA CGCGCTTCGT TCTACCATCG ACACCACACG CTGGCACCCA
  961 GTTGATCGGC GGCGGAAGAT TAATCGCCGC GACATTTGCG ACGCGGCGGT GCAGGGCAGA
 1021 CTGCAGGTGG CAACGCATCA GCACCGACTG TTTGCCGCAG TGTGTGCCAA GCGATGGATG
1081 TATCAGCTCC GCATGCCGAT CACTTTTCGC GATTCGCAGA TCGTGCCTGA CCT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]