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icon below as well as clone information before placing your order.
Expression clone for an auxin-based degron (AID) system, CENPH-aid.
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Other clones in our bank
External Database Gallus gallus CENPH Oryza sativa OsTIR1 Arabidopsis thaliana AtIAA17 Reference sequence |
| Sequence | RDB08470z.seq |
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| Remarks, protocol and/or map (pdf) | RDB08470.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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| Ordering | Order form [Credit Card Payment ] [Bank Transfer Payment]MTA, for use for not-for-profit academic purpose [Word ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA, 非営利学術目的用)[Word ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB08470 | pMK107 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pMK107 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08470). |
Reference section:
| Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., Kanemaki, M., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6(12), 917-922, (2009). PMID 19915560. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Auxin Inducible Degron (AID) System (English text) |
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| Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB08470_A5C2p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV-Forward Sequence file: RDB08470_A5C2a.seq
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>08470_08470_A5C2_CMV-Forward_A08_02.ab1 1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGATG 121 ACGTACTTCC CGGAGGAGGT GGTGGAGCAC ATCTTCAGCT TCCTGCCGGC GCAGCGCGAC 181 CGCAACACGG TCTCGCTCGT CTGCAAGGTG TGGTACGAGA TCGAGAGGCT GAGCCGCCGC 241 GGCGTCTTCG TGGGCAACTG CTACGCCGTG CGCGCCGGCC GCGTCGCCGC GCGGTTCCCC 301 AACGTGCGGG CGCTCACGGT GAAGGGGAAG CCCCACTTCG CCGACTTCAA CCTCGTGCCC 361 CCCGACTGGG GCGGCTACGC GGGGCCGTGG ATCGAGGCGG CCGCGAGGGG ATGCCACGGC 421 CTGGAGGAGC TCAGGATGAA GCGGATGGTG GTGTCCGACG AGAGCCTCGA GCTGCTGGCT 481 CGCTCGTTCC CGCGGTTCAG GGCTCTTGTT CTTATCAGCT GCGAGGGGTT CAGCACTGAC 541 GGGCTAGCCG CCGTCGCGAG CCATTGCAAG CTTCTGAGGG AGTTGGATTT GCAGGAAAAT 601 GAAGTGGAGG ATCGAGGGCC TAGGTGGCTT TCCTGCTTCC CTGATTCCTG CACATCACTT 661 GTCTCATTGA ATTTTGCCTG CATCAAAGGG GAGGTTAATG CTGGTTCACT GGAGAGACTT 721 GTTAGCAGGT CCCCAAACCT GCGGAGTTTG AGGCTGAATC GATCTGTATC GGTAGATACA 781 CTTGCAAAGA TACTACTGCG TACCCCTAAC TTGGAGGATT TGGGGACAGG GAATTTGACA 841 GATGACTTCC AAACTGAGTC CTACTTTAAG CTTACCAGTG CTCTGGAGAA ATGCAAGATG 901 TTGAGGAGTT TGTCTGGATT CTGGGATGCT TCTCCTGTTT GCCTGTCATT TATCTACCCC 961 CCTGTGTGCT CAACTGACAG GATTGAACTT GAGCTATGCA CCCACACTTG GATGCTTTCT 1021 GACCCTTACA AAAATGATTT AGCCGCTGTG TGAAGCTCCT ACCGACGTTT GGGTACTGCA 1081 TTGTAATCTC GACCAACGCC TGCCAGTTGT TGACCTCCAG CTTGCAAAGA ACCTTGGCCA 1141 G // |
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Primer: ME-1290Rv Sequence file: RDB08470_A5C2b.seq
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>08470_08470_A5C2_ME-1290Rv_B08_05.ab1 1 AACGCATTTC ATTTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA 61 GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCTA GAGTCGCGGC CGCTCAAGCT 121 CTGCTCTTGC ACTTCTCCAT CGCCCTCGGA GCGAGACCAA TGGCATCCGA TCCTTTCATG 181 AGACGTAAAC GCTTGCATGT ATCGACGAAC ATTGGCCAAG GAACGTCGCC GACGAGCATC 241 CAATCACCGT CTTTGTCTTC ATAAGAGGGA ACATAGTCCC AGCTATTCAC CAAATCCATC 301 AATTTCCTCT CATTCATGAA GTCTATCATT CCTTCTTCTC CTCCATGTTT GCCCATGGTA 361 AAAGAGCTGA ACATGTTGGA CAAAGCATTA GAAAGCTCAT CGTAGCTTTT ATACATCCTC 421 AAATCGATTT TCCTCAAGTA CGGTGCTCCG TCCATTGATA CCTTCACGAA CGCCGCCGCC 481 TCCGGGCCAC CGCTTGATTT TTGGCAGGAA ACCATCACGT TCTTCCGGTA TGATCTCACC 541 GGTGGCCATC CCACAACTTG TGCCTTGGCC GGAGGTTTGG CTGGATCTTT AGGACAAGCA 601 CTCTTCTCCT TGGAATCAAA AGTCACGACG TCATGAGTCG TAGATCCTTC CTTGTTTGCA 661 GGCTCATTAT TCAGATTTAG CTTCAGATCA ACCGTCTCTG AGAACCCTCT CTTGTTTCCG 721 GTTACCGGAG CCACTGTATC TCCACCGGGA AGACCAAGAC ACAGCTCAGT CTCCCTCAGA 781 TTCAGCTCGA CACTGCCCAT GGTTGTGGCG GCACCCGCTC CAGCGCCTGC ACCAGCTCCA 841 GTACTCAAGA GATTCTGAAT GGAGATATCT TTCTCGAGCT GTAGAATAAT TGCTCTTAAA 901 GATGGATCTT TCGCCCAGTT GATTTGGCAT CCGACTATGA TATTCTGGAG TATATTTTGA 961 ACTAACGTAG TCACCTTCCC TTTCATACTG TACATTCCTA TGGGATAAAC TTTCAACTTT 1021 TCAGCAATTT TCATTCTGAC TACTTCTTTT TTTTTTTCTC ACTGTGTAAA AACTCGCAAG 1081 CGCCTTCTTC TCCGTTCCGG ACTGTCAGAG TATCTTTCAT CTATGTCAAT TCCAAATTTC 1141 CTCTCTCTCT A // |
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Primer: IRES-R Sequence file: RDB08470_A5C2c.seq
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>08470_08470_A5C2_IRES-R_C08_08.ab1 1 GCATAGCGGC TTCGGCAGTA ACGTTAGGGG GGGGGGAGGG AGAGGGGCGG ATCCCGGGGA 61 TCTCTTGAAT GATCGTTCCA CTTTTTAGCT AGTGGATCCG TTCAAGTCTT CTTCTGAGAT 121 TAATTTTTGT TCACCGTTCA AGTCTTCCTC GGAGATTAGC TTTTGTTCAC CGTTCAAATC 181 TTCTTCAGAA ATCAACTTTT GTTCACCTCT AGAGGATCCG TTCAAGTCTT CTTCTGAGAT 241 TAATTTTTGT TCACCGTTCA AGTCTTCCTC GGAGATTAGC TTTTGTTCAC CGTTCAAATC 301 TTCTTCAGAA ATCAACTTTT GTTCACCTCT AGAGGATCCG TTCAAGTCTT CTTCTGAGAT 361 TAATTTTTGT TCACCGTTCA AGTCTTCCTC GGAGATTAGC TTTTGTTCAC CGTTCAAATC 421 TTCTTCAGAA ATCAACTTTT GTTCACCACT AGCAGCAGAA CCGGAGTCGA CTAGGATTTT 481 AACAAAATTT GGTGCATCAT CCCTCGCCCC AGCAGTTGTG CGGTACACAT ATAATTTCTC 541 CACTTTAGGC AGATCTCCAT GGTTTTCCTC CATTTCATTG CTACCATCCC GCTCATTTAT 601 GACCTCAACA TTGAGCATCG GCATCTTTGA CGCAAGGACT TGGCACCCCT TTAACGTGAC 661 ATTGCAAGAT GACATCCAAA GGGATCGCAT TGTCTCGTAC CTAGCAAAAT TCCCCAAGAG 721 TGCAGCATCA CCAAACGGGC TATCTCTTAT CTCCAGTTTC CTTAAATTCT TGCATCCATT 781 CATAACATGC ATCATACCCT TATCACTGTC ACCAGCAAAT GCTATAGAAA GCATCTCAAG 841 TTGTTTTGCA TATTTCCCAA TATACATGAA AACTTTGTCT GTGAGAAGAC CAGATATTGA 901 CAAACGTTGT AATCCCTTGC ACTCACGAAC AATAGCTCCA AAGCCTTCAT CTAATGGTTG 961 GCTTGTCACA ACATCAGGCT TCCCTGGCTC AAGAATACAA AGTCTGAATC GTGTGAAATT 1021 TGGGACAGTT CTTGGCGACA GTAACTAGTG CAGCATTTAG TCATTTGGGT GACAGAGTAC 1081 AGTAGTGAGT TCAGTTTTGC ACAGGCCAGA TACCTGCACA GTTCCCTTCC TCTGTCACTG 1141 CAGAAAATTA ACCCAAG // |
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Primer: IRES-F Sequence file: RDB08470_A5C2d.seq
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>08470_08470_A5C2_IRES-F_D08_11.ab1 1 CGGTCATTCT GTGTTTAGTC GAGGTTAAAA AACGTCTAGG CCCCCCGAAC CACGGGGACG 61 TGGTTTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGTGCGA TATCGAATTC 121 ACCATGGCGG GGCGGCTGTC GGAGTCCGTC GGGTCCGGGC CGGGCGCTGA GGCCGAGACG 181 GCGGCCGATC CGGACGCGAA GCGGGACGTC CTGGAGCACC TCTGCGCGAG GACGCATCTG 241 AAGCAGCTGG TGATGGAGTT CGACACCGCG TGCCCCCCCG ATGAAGGGTG CAACAGTGGC 301 GCGGAAGTAA ACTTTATCGA AAGTGCCAAA GAAACTTTGG AAGAAGTGGG GAAAGTAAAA 361 AGTGCCTTTG AAAGCAAAGC ATTAGTCATA AAAAGGATCC AGCTTATGGA TGCTCTGAGA 421 AAGAGAGTGA AAGAAAATGA TGGTTGCGCA CGACTGATCG TGGAAACAAT GAGAGACATA 481 ATCAAGCTCA ACTGGGAAAT AATTCAAGCT CATCAGCAAG CACGTGTGAT TAGAGAGAAT 541 TTGAATGACA TTAGAAGGAA AAGATACTTT CTGAAACAGG CCGAAGGAGA GAAGGCGCTG 601 CGAATTTTTA CCACAGTGAG GAAAAAAAAG GAAGTAGTCA GAATGAAAAT TGCTGAAAAG 661 TTGAAGTTTA TCCATAGGAA TGTACAGTAT GAAAGGAAGG TGACTACGTT AGTTCAAAAT 721 ATACTCCAGA ATATCATAGT CGGATGCCAA ATCAACTGGG CGAAAGATCC ATCTTTAAGA 781 GCAATTATTC TACAGCTCGA GAAAGATATC TCCATTCAGA ATCTCTTGAG TACTGGAGCT 841 GGTGCAGGCG CTGGAGCGGG TGCCGCCACA ACCATGGGCA GTGTCGAGCT GAATCTGAGG 901 GAGACTGAGC TGTGTCTTGG TCTTCCCGGT GGAGATACAG TGGCTCCGGT AACCGGAAAC 961 AAGAGAGGGT TCTCAGAGAC GGTTGATCTG AAGCTAAATC TGAATAAATG AGCCTGCAAA 1021 CAAGGAAAGG ATCTACGACT CATGACGTCG TGACTTTTTG ATTTCGACGA GAGAGTGCTG 1081 TCCTAAAGAT TCAGCGAACT CGGACAGCAC AGCTGTGGGA ATGGCACGTG GAATCATACT 1141 GGCAGCAACC GAG // |
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Primer: SV40pro_F_V2 Sequence file: RDB08470_A5C2e.seq
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>08470_08470_A5C2_SV40pro_F_V2_E08_14.ab1 1 ACCATGGCAC TCTTTTGAGG CTAGGCTTTT GCAAGATCGA TCAAGAGCAC ACGGATGAGG 61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG 601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 961 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 1021 GAGTTCTTCG CCACCCTAGG GGGAGGCTAA CTGAACACGG AAGAGACATA CCGGAGCACC 1081 CGCCGCTTAT GACGGCAATT AAAAGACCGG AATAAAACGC CACGGTGTTG GGATCGGATT 1141 TGAATCTA // |
Please visit 
Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 6(12), 917-922, (2009). PMID 19915560. [link to RRC of NBRP] |
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| reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
| reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
| reference | Holland, A.J., Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 109 (49): E3350-7 PMID 23150568. |
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