RDB08470 - pMK107

pMK107 (#RDB08470)

Expression clone for an auxin-based degron (AID) system, CENPH-aid.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Remarks, protocol and/or map (pdf)	RDB08470.pdf
Publication	Nishimura, K., Nat Methods 6(12), 917-922, (2009). PMID 19915560. [PubMed] [Article] [RRC of NBRP]
Test sheet	Data Sheet open

Alternative name	OsTIR1-9Myc::IRES::CENPH-aid (kan/neoR)
Clone info.	Expression clone for an auxin-based degron (AID) system, CENPH-aid. Refer Supplementary figure 11 (CENPH-aid) of Nat. Methods, 6, 917-922, 2009; PMID: 19915560.
Comment	Although this clone is constructed on the pIRES-EGFP, it doesn't contain GFP. Accession numbers, AtIAA17 (GeneID 839568), CENPH (Gallus gallus). To make 0.5 M stock solution, dissolve indole-3-acetic acid (IAA) (Sigma-Aldrich, 45533) or 1-naphthaleneacetic acid (NAA) (Sigma-Aldrich, 35745) in 100 % DMSO. IAA solution can be made in 100 % ethanol. Aliquots can be kept for 6 months in the dark at -20oC (Devrekanli, A., Kanemaki, M.T. Methods in Molecular Biology, 1369, 257-278, 2016).
Vector backbone	pIRES-EGFP (Plasmid)
Selectable markers	Kan^r
Growth conditions	37C, LB+Kan
Gene/insert name	Gallus gallus CENPH cDNA Oryza sativa OsTIR1 Arabidopsis thaliana AtIAA17
Depositor\|Developer	Kanemaki, Masato \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Nishimura, K., Nat. Methods., 6, 917-922 (2009)). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishimura, K., Nat. Methods., 6, 917-922 (2009))。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。

Catalog #	Resource name	Shipping form	Fee
RDB08470	pMK107	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMK107 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08470).

Reference section:

Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T., Kanemaki, M., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6(12), 917-922, (2009). PMID 19915560. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Nishimura, K., An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods 6(12), 917-922, (2009). PMID 19915560. [PubMed] [Article] [RRC of NBRP]
reference	Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. [PubMed] [Article]
reference	Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. [PubMed] [Article]
reference	Holland, A.J., Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 109 (49): E3350-7 PMID 23150568. [PubMed] [Article]