Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-CMV-mRFP1-RfA (#RDB08492)

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by CMV promoter.

Clone info.	Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by CMV promoter.
Comment	Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging.
Vector backbone	Plasmid
Selectable markers	Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions	37C, LB+Amp
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer	Miyoshi, Hiroyuki |

 

Sequence (full)	RDB08492zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB08492.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08492	CSII-CMV-mRFP1-RfA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The CSII-CMV-mRFP1-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08492).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12133_A2E1p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-check_R (Pr0521) Region: mRFP1 5', CMV pro_2, CMV en_2 Sequence file: RDB12133_A2E1a.seq
>08492_12133_B1K2F_mCherry_check_R_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNCNNCCNC NNTCCTCNNA GTTCNTCACG CGCTCCCACT TGAAGCCCTC    61 GGGGAAGGAC AGCTTCAAGT AGTCGGGGAT GTCGGCGGGG TGCTTCACGT AGGCCTTGGA   121 GCCGTACTGG AACTGAGGGG ACAGGATGTC CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT   181 CACCTTCAGC TTGGCGGTCT GGGTGCCCTC GTAGGGGCGG CCCTCGCCCT CGCCCTCGAT   241 CTCGAACTCG TGGCCGTTCA CGGAGCCCTC CATGCGCACC TTGAAGCGCA TGAACTCCTT   301 GATGACGTCC TCGGAGGAGG CCATCGGATC CAGTCGAGAC CGGTAGCGCT AGCGGATCTG   361 ACGGTTCACT AAACCAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT   421 TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA   481 AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG   541 CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA   601 TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAANGNC ATGTACTGGG CATAATGNCN   661 GGCGGGCNNT TTACNGTCNT TGACGTCNAN AGGGGGNGTA CTTGGNATAN GANACNNNNN 721 NNGNNCNNNC NNNNNNGGNN NNNTTNNN //
Primer: PRE_rev (Pr0250) Region: PRE, mRFP1 3' Sequence file: RDB12133_A2E1b.seq
>08492_12133_B1K2F_PRE_rev_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGNN NNNNNNCAAC ATAGTTAAGA ATACCAGTCA ATCTTTCACA    61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG   121 GATCCGCGGC CGCGAATTCT TAGGCGCCGG TGGAGTGGCG GCCCTCGGCG CGCTCGTACT   181 GTTCCACGAT GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAGCTTGATG TCGGTCTTGT   241 AGGCGCCGGG CAGCTGCACG GGCTTCTTGG CCATGTAGGT GGTCTTGACC TCGGCGTCGT   301 AGTGGCCGCC GTCCTTCAGC TTCAGCCTCA TCTTGATCTC GCCCTTCAGG GCGCCGTCCT   361 CGGGGTACAT CCGCTCGGTG GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC   421 CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA   481 GGGAGGAGTC CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC   541 ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GANGTCGGCG GGGTGCTTCA   601 CGTANGCCTT GNAGCCGTAC TGGAACTGAN GGGACAGGAN GTCCCAGGCN AANGGGNAGG   661 GGGCCGCCCT TGNTCACCTT NNGCTTNNNN GTCTGGGNGN CNTNNNNNNG NNGNNCCTCG 721 CCCTCNNCCN NNNNNTCNNN NNNNNNN //
Primer: Cm_N_rev (Pr0014) Region: CmR, attR1, PRE Sequence file: RDB12133_A2E1c.seq
>08492_12133_B1K2F_Cm_N_rev_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTGG GNNNTATCAA CGGTGGTATA TCCNAGTGAT    61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GACGGATCCT AACTCAAAAT   121 CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGCGGCCGC   181 CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC   241 TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA   301 CTTGTGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT   361 AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT   421 GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG   481 GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA   541 TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAN GGCGAANGCG AAGACGCGGA   601 ANANGCCGCA NANCCGGCAG CANGCCGCGG GGAAGGAAGG TCCGCTGGAN TGANGGCCNA   661 NNGGACGTAN CANANNNNNG TCCCNCGCAN AATCCNNNTG GCNNCNCNNN CNANNANNCN 721 NNGAANNNNN NNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA, 3'LTR, attR2, ccdB Sequence file: RDB12133_A2E1d.seq
>08492_12133_B1K2F_bGH_rev3_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNAGAAGGCA CAGTCGAGGC TGATCAGCGG GTTTAAACGG    61 GCCCTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA GTTACCAGAG   121 TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA TTGAGGCTTA   181 AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC TAACCAGAGA   241 GACCCAGTAC AGTCCGGATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA ACGTAAAATG   301 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA   361 AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT   421 GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG   481 CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC   541 ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGNNTTTA   601 TCTGACAGCA GACGTGCACT GGCCNGGGGG NNCANCATCC GTCNCCNGGG NGTGTCNATA   661 ATATCACTCT GNNCATCCNN NAANNGACNA NNNNNGNTCT CNCNTTTNNN NNNGGNNNNN 721 ANNNTNNANN NNNNNNTTNN NNNNNNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi, 5'LTR, CMV pro_1, CMV en_1 Sequence file: RDB12133_A2E1e.seq
>08492_12133_B1K2F_HIV-B_R_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCANC NAGCCGAGTC    61 CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC   121 TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC   181 ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA   241 GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC   301 CAGTACAGGC AAAACGCGCT GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT   361 TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA   421 AACAAACTCC CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC   481 TATCCACGCC CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT   541 ACGTAGATGT ACTGCCAAGT ANGAAAGTCC CATAANGTCA TGTACTGGGC ATAATGNCAG   601 GCGGGCCATT TACCGTCATT GACGTCNATA NGGGGCGTAC TTGNCATATG ANACACTNNA 661 NGNACTGNNN NNNNNNN //
Primer: cPPT/CTS-E_R (Pr0725) Region: cPPT_CTS Sequence file: RDB12133_A2E1f.seq
>08492_12133_B1K2F_cPPT_CTS-E_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNTATT ANNTCTACTA TTCTTTCCCC TGCACTGTAC CCCCCAATCC    61 CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG TAGATCCGTT CACTAATCGA   121 ATGGATCTGT CTCTGTCTCT CTCTCCACCT TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT   181 CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC GATAATGGTG AATATCCCTG CCTAACTCTA   241 TTCACTATAG AAAGTACAGC AAAAACTATT CTTAAACCTA CCAAGCCTCC TACTATCATT   301 ATGAATAATT TTATATACCA CAGCCAATTT GTTATGTTAA ACCAATTCCA CAAACTTGCC   361 CATTTATCTA ATTCCAATAA TTCTTGTTCA TTCTTTTCTT GCTGGTTTTG CGATTCTTCA   421 ATTAAGGAGT GTATTAAGCT TGTGTAATTG TTAATTTCTC TGTCCCACTC CATCCAGGTC   481 GTGTGATTCC AAATCTGTTC CAGAGATTTA TTACTCCAAC TAGCATTCCA AGGCACAGCA   541 GTGGTGCAAA TGAGTTTTCC AGAGCAACCC CNAATCCCCA GGANCTGTTG ATCCTTTANG   601 TATCTTTCCN CAGCCAGGAN TCTTGCCTGG ANCTGCTTGA TGCCCCAGAC TGNGANTTGC   661 NNNNNANGCT GNTNNGCCTC NNNANCCCTN NNCNANNNGN TCTGCTGCTG NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, Zeo Sequence file: RDB12133_A2E1g.seq
>08492_12133_B1K2F_SV40pro_ori_F_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNGNNN TNNTCNNGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG    61 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT   121 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA   181 ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG   241 GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC   301 GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG   361 GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG   421 GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG   481 CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG   541 GCCGANGAGC AGGACTGACA CGTGCTACGA GATTTCGATT CCNCCGCCGC CTTCTATGAA   601 AGGNTGGGCT TCNGANNCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCN GCGCGGGGNT   661 CTCNTGCTGG AGNNCNTCNC CNNCCCCNNC NNNNTNANNG NNNCTTNNNN NNNNTNNNNN 721 NNANNNNNNN NNN //
Primer: RRE_F1 (Pr0796) Region: RRE Sequence file: RDB12133_A2E1h.seq
>08492_12133_B1K2F_RRE_F1_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTTGNCTG NNGGCTATTG    61 NNGCNNNNNN NTCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC   121 CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA   181 AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA   241 CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC   301 TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA   361 TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG   421 TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT   481 GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC   541 CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAANG TGGAGAGAGA   601 GACAGAGACA GATCCNTTCG ATTAGTGAAC GGATCTACAA ATGGCAGTAT TCNTCCNCAA   661 TTNNAAAAGA AAANGGGGGN NNGGGGGGGN ACAGTGCNGG GNNAANNATA GTAGANNTNN 721 NAGCNNNNGA NNTNCNANCT NAANNNNNNN NAAANNNANN NNNNAAAANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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