Resource data sheet
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pPyCAG-EGFP-IP (#RDB08525)

Expression clone of GFP under the control of CAG promoter, puromycin resistance.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name stock no 627
Clone info. Expression clone of GFP under the control of CAG promoter, puromycin resistance.
Comment SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Amp^r
Growth conditions 37 C, LB + Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee
RDB08525 pPyCAG-EGFP-IP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pPyCAG-EGFP-IP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08525).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

reference Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351. [PubMed] [Article] [RRC of NBRP]