Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-EGFP-IP

Expression clone of GFP under the control of CAG promoter, puromycin resistance.

Sequence	RDB08525z.seq
Remarks, protocol and/or map (gif)	RDB08525a.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08525_A3L4p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: EGFP 5' Sequence file: RDB08525_A3L4c.seq
>08525_08525_A3L4_pAxCA_F1_G05_20.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCTT CTTCTTTTTC CNNCNGCTCC TGGGCAACGT    61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC CTCGAGATCC ATTGTGGCCA   121 GTGCGGCCGA TCCCCGGGTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA   181 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG   241 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA   301 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC   361 AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC   421 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC   481 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG   541 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA   601 ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC   661 ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG   721 GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN CACCCAGTCC GCCCTGAGCA   781 AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA NTTCGTGACC GCCGCCGGGN   841 TCACTCTCGG CATGGNCGAN CTGTACAAGT AAAGCGGCCG GCCGCNCTGG NNNCAANGGN   901 TCTCGANGAN NTNNNTCCNC NNNNGCNNGN TGCCNNTCAN NANNNGGNGG CNNNNGNNNC 961 CNNNNGGNNN NNNANNNNNN NNNN //
Primer: IRES-R (Pr0037) Region: EGFP 3' Sequence file: RDB08525_A3L4d.seq
>08525_08525_B0F9F_IRES_R_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTTANGGG GGGGGGNNGN NNNNGGGGGG GGGGCGGAAT    61 TCGATATCAA GCNNNNNNAG CGGCCGCTCG ATACCGTCGA TCTCAGTGGT ATTTGTGAGC   121 CAGTTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTGAATTCC   181 TCGAGATCCA TTGTGGCCAG TGCGGCCGGC CGCTTTACTT GTACAGCTCG TCCATGCCGA   241 GAGTGATCCC GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG   301 GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC   361 CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA   421 TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT   481 AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT   541 TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT   601 CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC   661 CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TANCGGCTGA   721 AGCACTGCAC GCCGTANGTC ANGGNGGTCA CGANGGTGGG CCAGGGCACN GGCAGCTTGC   781 CGGTGGTGCA GATGAANCTT CAGGGTCAGC TTGCCGTNNG NGGCATCNCC CTCNNCCNCN   841 CNNGNANACG CTGNNCTTGT GGNCGTTTNN NNCNNCNNNC NGCNCGNNNN NNNNGGGNNC 901 CACCCCNGNN NANNNNCTNC NTCNNCNNTG CNNNANCNAN GGNNGNNNNN NNNNN //
Primer: Amp_R (Pr0008) Region: polyoma ori,SV40 p(A),HCMVIEE Sequence file: RDB08525_A3L4e.seq
>08525_08525_B0F9F_3_Amp_R_H06_24_ABI24.ab1     1 NNNNNNNNNN NNNNGCTTTG GNANNNNNAG ANTGTATTTC CTGGAAATTA ATGTTTATTC    61 NATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG   121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC   181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC   241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG   301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG   361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT   421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT   481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA   541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG   601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT   661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT   721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT   781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGNCCGCCTG   841 GCTGANCGCC NNACGACCCC CGCCNATTGA CGTCAATNAT GACGTANGTN CCCNTANNAA 901 CGCCNATANG GNNTTTNCAT NNNNNNNNNN //
Primer: IRES-F (Pr0036) Region: IRES, PuroR Sequence file: RDB08525_A3L4f.seq
>08525_08525_A3L4_IRES-F_H05_23.ab1     1 NNNNNNNNNN NNNNTTNNGT CGANGNNNNN NAACGTCTAG GCCCCCCGAA CCNCGGGGNN    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC   121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC   181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT   241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT   301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCNCGCCG GANANCGTCG AANCGGGGGG   361 CGGNGTTCGC CGANATCGGC CCGCGCNTGG NCGANTNNAN CNGNTTNCCG GNTNGNNCNC 421 NCNNNNANNN NNNNNNANNN CNNCNGGNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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