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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPyCAG-EGFP-IZ (#RDB08526)

Expression clone of GFP under the control of CAG promoter, zeocin resistance.

Alternative name stock no 1256
Clone info. Expression clone of GFP under the control of CAG promoter, zeocin resistance.
Vector backbone pPyCAG (Plasmid)
Size of vector backbone 6.4 kb
Selectable markers Amp^r
Growth conditions 37 C, LB + Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB08526z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Cell, 123, 917-929 (2005)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cell, 123, 917-929 (2005))。
付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08526 pPyCAG-EGFP-IZ DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pPyCAG-EGFP-IZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08526).

Reference section:

Niwa, H., Toyooka, Y., Shimosato, D., Strumpf, D., Takahashi, K., Yagi, R., Rossant, J., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08526_A8Etp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB08526_A8Eta.seq check
>08526_08526_A8Et_pAxCA_F1_F04_16_ABI24.ab1
    1 CATCCCTGTC TACATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCCTCG AGATCCATTG TGGCCAGTGC
  121 GGCCGATCCC CGGGTACCGG TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG
  181 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC
  241 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC
  301 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG
  361 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA
  421 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC
  481 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT
  541 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT
  601 CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA
  661 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA
  721 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA
  781 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
  841 TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGGCCG CACTGGCCAC AATGGATCTC
901 GAGGAATTCC GCCCCCCCCC CCCCCCTTCT CCC
//
Primer: IRES-R
Sequence file: RDB08526_A8Etb.seq check
>08526_08526_A8Et_IRES-R_G04_19_ABI24.ab1
    1 GCAAGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGCGGAATTC
   61 CTCGAGATCC ATTGTGGCCA GTGCGGCCGG CCGCTTTACT TGTACAGCTC GTCCATGCCG
  121 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
  181 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG
  241 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG
  301 ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA
  361 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC
  421 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC
  481 TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG
  541 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG
  601 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG
  661 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC CTCGCCCTCG
  721 CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
  781 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGTGG CGACCGGTAC CCGGGGATCG
  841 GCCGCACTGG CCACAATGGA TCTCGAGGAA TTCTTTGCCA AAATGATGAG ACAGCACAAT
  901 AACCAGCACG TTGCCCAGGG AGCTGTAGGA AAAGAAGAAG GCATGAACAT GGTTTAGCAG
  961 AGGCTCTAGA GCCGCCGGTC ACACGGCCAG AGCGAAACCC CGCCTGCCCC GTCCCCCCGG
 1021 AAGCAGCCGT CCCCCCGCGG ACCGCCCGGA GCTGGAGAAG GAGAGGGACG CGGCCGCGAC
1081 GCACGAAGGC CATTG
//
Primer: pCA-bgter
Sequence file: RDB08526_A8Etc.seq check
>08526_08526_A8Et_pCA-bgter_H04_22_ABI24.ab1
    1 AAAATGGGGG TCCCCATAAA TTTTTTGGCA GAGGGAAAAG ATCGAACCCC CCTCGGAACC
   61 CGTCGGGCCG CGTCGGACCG GCGGTGTTGG TCGGCGTCGG TCAGTCCTGC TCCTCGGCCA
  121 CGAAGTGCAC GCAGTTGCCG GCCGGGTCGC GCAGGGCGAA CTCCCGCCCC CACGGCTGCT
  181 CGCCGATCTC GGTCATGGCC GGCCCGGAGG CGTCCCGGAA GTTCGTGGAC ACGACCTCCG
  241 ACCACTCGGC GTACAGCTCG TCCAGGCCGC GCACCCACAC CCAGGCCAGG GTGTTGTCCG
  301 GCACCACCTG GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG ACCACACCGG
  361 CGAAGTCGTC CTCCACGAAG TCCCGGGAGA ACCCGAGCCG GTCGGTCCAG AACTCGACCG
  421 CTCCGGCGAC GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG GCCATGGTTG
  481 TGGCCATATT ATCATCGTGT TTTTCAAAGG AAAACCACGT CCCCGTGGTT CGGGGGGCCT
  541 AGACGTTTTT TTAACCTCGA CTAAACACAT GTAAAGCATG TGCACCGAGG CCCCAGATCA
  601 GATCCCATAC AATGGGGTAC CTTCTGGGCA TCCTTCAGCC CCTTGTTGAA ATACGCTTGA
  661 GGAGAGCCAT TTGACTCTTT CCACAACTAT CCAACTCACA ACGTGGCACT GGGGTTGTGC
  721 CGCCTTTGCA GGTGTATCTT ATACACGTGG CTTTTGGCCG CAGAGGCACC TGTCGCCAGG
  781 TGGGGGGGTT CCGCTGCCTG CAAAGGGTCG CTACAGACGT TGTTTGTCTT TCAGAAGCTT
  841 TCCAGAGGAA CTGCTTCCTT CACGACATTC AACAGACCTT TGCATTCCTT TGGCGAGAGG
  901 GGAAAGACCC CCTAAGGAAA TGCCTCGTCA AAGAAGAACA GGGCCCAAGG TTTTCCGGTC
  961 CTCACATGCC CAAAAGAACG CATATGGTGA AAATAACATA ATAGGATAGC GGCACACGCT
1021 TATTCCAAGC GCTCGTCAGT TAACGTTAAG GGGAAAGGGG AAGAGG
//
Primer: SV40pA3'element Reverse
Sequence file: RDB08526_A8Etd.seq check
>08526_08526_A8Et_SV40pA3'ElementReverse_A05_02_ABI24.ab1
    1 GTGCTTGGGG GGCGGACCTA TGGAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
   61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
  121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
  181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
  241 TGGCCGATTC ATTAATGCAG CGGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA
  301 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
  361 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAGC TTTTTGCAAA AGCCTAGGCC
  421 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC
  481 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT
  541 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCGG
  601 ATCCGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
  661 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG
  721 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC
  781 GCCAAGCTTG GGCTGCAGGT CGAGGGATCT CCATAAGAGA AGAGGGACAG CTATGACTGG
  841 GAGTAGTCAG GAGAGGAGGA AAAATCTGGC TAGTAAAACA TGTAAGGAAA ATTTTAGGGA
  901 TGTTAAAGAA AAAAAAATAA CACAAAAACA AAATATAAAA AAAATCTAAA CCCTCAAAGT
  961 CAAAGGCTTT TCTATGGGAA ATAAAGAAAT GGGACAGCAC GGGGGCTGTT TTCATATACT
 1021 GATGGACCCT CTTTATAGCC CAACCCTTTG TTTCATTGGC AGGTCAGGCA TATGGGGTCA
1081 TATGGTGCTA ACTTCTAAAC CAAAATACTC CATTTCCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Niwa, H., Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. Cell, 123, 917-929 (2005). PMID 16325584. [link to RRC of NBRP]
reference Tran, T.H.Y., Live cell imaging of X chromosome reactivation during somatic cell reprogramming. Biochem. Biophys. Rep. 15: 86-92 (2018). PMID 30094351. [link to RRC of NBRP]
user_report Ohhata, T., Histone H3 lysine 36 tri-methylation is established over the Xist promoter by antisense Tsix transcription and contributes to repressing Xist expression. Mol. Cell. Biol. 35 (22): 3909-3920 (2015). PMID 26370508. [link to RRC of NBRP]