Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBRPyCAG-EGFP-IN

Expression clone of GFP under the control of CAG promoter, neomycin resistance

Sequence	RDB08530z.seq
Remarks, protocol and/or map (gif)	RDB08530a.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB20093_B3Iqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584) Region: CAG pro,EGFP 5' Sequence file: RDB20093_B3Iqa.seq
>08530_20093_B3Iq_1_CAG_R1_F02_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCCCNNCNNN CGGGCGCGGG GCNNANCGGT GCGGCGCCGG    61 CAGGNAGGAA ATGGGCGGGG NGGGCCTTCG TGCGTCGCCG CGCCGCCGTC CCCTTCTCCC   121 TCTCCAGCCT CGGGGCTGTC CGCGGGGGGA CGGCTGCCTT CGGGGGGGAC GGGGCAGGGC   181 GGGGTTCGGC TTCTGGCGTG TGACCGGCGG CTCTAGAGCC TCTGCTAACC ATGTTCATGC   241 CTTCTTCTTT TTCCTACAGC TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT   301 TGGCAAAGAA TTCCTCGAGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG   361 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC   421 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC   481 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC   541 AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC   601 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG   661 GTGAAGTTCG AGGGCGACAC CCTGGTGAAN CGCATCGAGC TGAANGGCAT CGACTTCNAG   721 GAGGANGGCA ACNTCCTGGG GCACAAGCTG GANTACAACT ACAACAGCCA CAACGTCTAT   781 ATCATGGNCG ACAAGCAGAA NAACGGCATC NNNNTGANCT TCNAGATNCG NCACNNCNTC   841 GANGANNGCA GCGTGNANCN NNNNNANCAN TANCNNCANA NCANCCCCCA TCGGCGANNN   901 NNNNNNGCNN CTGCCCNNNN NNNCNNNNAN CNNNANCANC CANNNCNNNC NNNNNNNNNN //
Primer: EGFP-N_F2 (Pr1228) Region: EGFP 3',IRES Sequence file: RDB20093_B3Iqb.seq
>08530_20093_B3Iq_1_EGFP-N_F2_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNC NNCNTCTTCT TCAGGNNGAN NGCAACTACA AGNCCCGCGC    61 CGNNGNGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT   121 CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT   181 CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA   241 CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA   301 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA   361 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC   421 TCTCGGCATG GACGAGCTGT ACAAGTAAGC GGCCGCTCGA TAAGCTTGAT ATCGAATTCC   481 GCCCCCCCCC CCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA   541 GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG   601 AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGNATTC CTAGGGGTCT TTCCCCTCTC   661 GCCNAAGGAA TGCAANGTCT GTTGAATGTC GTGANNNANN NNNTTCCTCT GGAAGCTTCN   721 TGAANANNAN CNANNNNNCT GTAGCGACCC NTTGCANGCA NNNGNAANCC CCCNNNCNNG   781 NNNNNCNNNN NGNCTCTGNN GGCCNNAAAG NCCNNGTGNA NNANNNANNN NNNTGCNNNN   841 NCGGNNNNNN CCNCNNNNGN CCNNNNNTNN NGNANTTNNN NNNNNTNNNN GNNNNNNANT 901 NNANATGNNN NNNNNNN //
Primer: Amp_R (Pr0008) Region: Polyoma ori, SV40 pA Sequence file: RDB20093_B3Iqc.seq
>08530_20093_B3Iq_1_Amp_R_H02_08_ABI08.ab1     1 NNNNNNNNNN NNNNNCTTNN GNNNNNNAGN NTGTANTTCC TGGNANTTAA TGTTTATTCA    61 ATAAACTGTG NATTCAGCTA TATTCACATA GTGGTGAGGC TGAAATGAGG CGGGAAGAGG   121 CGGTTGGGGC TTAATTATAT CAATTTGGGT GGCCCCACAG CGCCTCCAAG GCGCCAGTCC   181 TGTTTTGACA AGTTGCCTCT GGAAGCCTCT CTTCTTTTTC TCCAGAGTAA GCGGAGGCCA   241 GGGGCCCCCG GCCTCTGCTT AATACTAAAA AAAACAGCTG TTGTCATAGT AATGATTGGG   301 TGGAAACATT CCAGGCCTGG GTGGAGAGGC TTTTTGCTTC CTCTTGCAAA ACCACACTGA   361 CATTCCAGGC CTGGGTGGAG AGGCTTTTTG CTTCCTCTTG CAAAACCACA CTGCCCTCTG   421 GAGGGCAGTT GCCTAGCAAC TAATTAAAAG AGGATGTCGC ACGGCCAGCT GCGGTCAGTT   481 AGTCACTTCC TGCTTAACTG ACTTGACATT TTCTATTTTA AGAGTCGGGA GGAAAATTAC   541 TGTGTTGGAG GCCCTCCGCC ATCTTCTGAA GCTGAATCGA ATTAACTTGT TTATTGCAGC   601 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC   661 ACTGCATTCT ANTTGTGNTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG   721 ANATCATCGT CGACATTGAT TATTGACTAN CTAGTTATTA ATAGTAATCA ATTACGGNNT   781 CATTAGTTCA TANCCNATAT ATGGANTTNC GCGTTACATA ACTNACNGNA AATGGCCCGC   841 CNNGCTGANC GCCCAACNNN CCCCNCCNNT TNNCGTCNAT NNNNNNNNAT GNNNCNATAN 901 NAANNNCNNN TANGNNNNTN NNNNNNNNN //
Primer: IRES-F (Pr0036) Region: IRES,NeoR 5' Sequence file: RDB20093_B3Iqd.seq
>08530_20093_B3Ig_1_IRES-F_D02_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNTNN NTCGNNGTTA NNANAACGTC TAGGCCCCCC NAACCACGGG    61 GNNGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGA TTGAACAAGA   121 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC   181 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC   241 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC   301 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC   361 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC   421 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC   481 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG   541 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT   601 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGNTCTCGT   661 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGNNC GCTTTTCTGG   721 ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CAGGACATAN CGTTGGCTAC   781 CCGTGATATT GCTGAAGANC TTGNCGGCGA ATGGGCTGAN CGCTTCCTCN NGCTTTACNG   841 NATCGCCGCN NCNGANTCNC ANCGCATCGC CNTCTATCGC CTNNTTGANN ANTNCNNNTG 901 ANGGNNNCNG NNNNTAAAAA NANNGAANNA AANNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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