RDB08716 - pYTY103

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pYTY103 (#RDB08716)

Plasmid clone to express ERG6 fused with mRFP in S cerevisiae

Alternative name	172
Clone info.	Plasmid clone to express ERG6 fused with mRFP under its own promoter in S cerevisiae.
Comment	ERG6-mRFP on pRS316 (pCEN, URA3)
Vector backbone	pRS316 (Plasmid)
Selectable markers	Amp
Gene/insert name	S. cerevisiae ERG6
Depositor\|Developer	Nakano, Akihiko \|

Sequence	RDB08716z.seq
Remarks, protocol and/or map (pdf)	RDB08716.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Biochem. 143, 803-811, 2008).

Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Biochem. 143, 803-811, 2008).
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biochem. 143, 803-811, 2008)。
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08716	pYTY103	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYTY103 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08716).

Reference section:

Takeda, Y., Nakano, A., In vitro formation of a novel type of membrane vesicles containing Dpm1p: putative transport vesicles for lipid droplets in budding yeast. J. Biochem. 143, 803-811 (2008). PMID 18343875. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08716_A8Lcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH1pro_F () Sequence file: RDB08716_A8Lca.seq
>08716_08716_A8Lc_3_ADH1pro_F_A07_01_ABI24.ab1 1 GTCCAGTTTA TTTAATCTTT TGTTTCCTCG TCATTGTTCT CGTTCCCTTT CTTCCTTGTT 61 TCTTTTTCTG CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTATC TCATATACAG 121 AATTCTTCGG GTGTTTTCTC CTATCCTCTG CTGCTCTCTT TTTCTTTCCA TTCGTCTTTG 181 TAATTTATAA GCTGTTAATT TTCATTCAAG TTTCCCTTAT CTGTTTTACT TTCGATTTAA 241 GTTTTACATA ATTTAAAAAA ACAAGAATAA AATAATAATA TAGTAGGCAG CATAAGATGA 301 GTGAAACAGA ATTGAGAAAA AGACAGGCCC AATTCACTAG GGAGTTACAT GGTGATGATA 361 TTGGTAAAAA GACAGGTTTG AGTGCATTGA TGTCGAAGAA CAACTCTGCC CAAAAGGAAG 421 CCGTTCAGAA GTACTTGAGA AATTGGGATG GTAGAACCGA TAAAGATGCC GAAGAACGTC 481 GTCTTGAGGA TTATAATGAA GCCACACATT CCTACTATAA CGTCGTTACA GATTTCTATG 541 AATATGGTTG GGGTTCCTCT TTCCATTTCA GCAGATTTTA TAAAGGTGAG AGTTTCGCTG 601 CCTCGATAGC AAGACATGAA CATTATTTAG CTTACAAGGC TGGTATTCAA AGAGGCGATT 661 TAGTTCTCGA CGTTGGTTGT GGTGTTGGGG GCCCAGCAAG AGAGATTGCA AGATTTACCG 721 GTTGTAACGT CATCGGTCTA AACAATAACG ATTACCAAAT TGCCAAGGCA AAATATTACG 781 CTAAAAAATA CAATTTGAGT GACCAAATGG ACTTTGTAAA GGGTGATTTC ATGAAAATGG 841 ATTTCGAAGA AAACACTTTC GACAAAGTTT ATGCAATTGA GGCCACATGT CACGCTCCAA 901 AATTAGAAAG GTGTATACAG CGAAATCTAC AAGGTTTTGA AACCGGGTGG TACCTTTGCT 961 GTTTACGAAA TGGGTAATGA CTGATAAATA TGACGAAAAA CATCCTGAAC ATAGAAAGAT 1021 CGCTTATGAA ATTGAACTAG GTGAATGGTA ATCCCAAGAT GTTCCATGTC GGACGTTGCT 1081 TAGGAAGCAA TTGAAGAACC CTGGTGGGTT C //
Primer: CMK1ter_R () Sequence file: RDB08716_A8Lcb.seq
>08716_08716_A8Lc_3_CMK1ter_R_B07_04_ABI24.ab1 1 TGGTTCCCGG GTTGTGTCAG GGCATGCACT GTGTTAACTA ATTGTGCAAA AGCTTAGATC 61 CTTAGGCGCC GGTGGAGTGG CGGCCCTCGG CGCGCTCGTA CTGTTCCACG ATGGTGTAGT 121 CCTCGTTGTG GGAGGTGATG TCCAGCTTGA TGTCGGTCTT GTAGGCGCCG GGCAGCTGCA 181 CGGGCTTCTT GGCCATGTAG GTGGTCTTGA CCTCGGCGTC GTAGTGGCCG CCGTCCTTCA 241 GCTTCAGCCT CATCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG 301 TGGAGGCCTC CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAG GGGAAGTTGG 361 TGCCGCGCAG CTTCACCTTG TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA 421 CGGTCACCAC GCCGCCGTCC TCGAAGTTCA TCACGCGCTC CCACTTGAAG CCCTCGGGGA 481 AGGACAGCTT CAAGTAGTCG GGGATGTCGG CGGGGTGCTT CACGTAGGCC TTGGAGCCGT 541 ACTGGAACTG AGGGGACAGG ATGTCCCAGG CGAAGGGCAG GGGGCCGCCC TTGGTCACCT 601 TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG GGCGGCCCTC GCCCTCGCCC TCGATCTCGA 661 ACTCGTGGCC GTTCACGGAG CCCTCCATGC GCACCTTGAA GCGCATGAAC TCCTTGATGA 721 CGTCCTCGGA GGAGGCCATA GATCTTTGAG TTGCTTCTTG GGAAGTTTGG GAGGGGGTTT 781 CGGCGTTTTC TGGCTTCCTA GCGACGAAAA GCATCATTGG AGTGAATAAC TTGGACTTAC 841 CACCGGCAAC TAAACCAACC GCAGCATTTT CTAGAGCAGC AGTAACTTCC TTGGAACCTT 901 CTGGGGGCTA GACCTAATTT TTTCCATTAC AGTAACCATT GCTGTAGTAA ATTGTCTACC 961 CAAGTAAGAA GTTTCTGAAA AATGTGGGCC CAAATTTAGC TAAAGTTTTG AACGTACTTT 1021 CCACTCACAG TAATTGGGGT TAATACCAAC GGATTCAATC AATCATGGTT CGTCAAGGTC 1081 CACGCTTAAC GGAAGGAACT TCGAAACCCA CCAGGGTC //
Primer: URA3pro_F () Sequence file: RDB08716_A8Lcc.seq
>08716_08716_A8Lc_3_URA3pro_F_C07_07_ABI24.ab1 1 TAAAAGGCTA GTGTTGAGAC ATGAAATTGC CCAGTATTCT TAACCCAACT GCACAGAACA 61 AAAACCTGCA GGAAACGAAG ATAAATCATG TCGAAAGCTA CATATAAGGA ACGTGCTGCT 121 ACTCATCCTA GTCCTGTTGC TGCCAAGCTA TTTAATATCA TGCACGAAAA GCAAACAAAC 181 TTGTGTGCTT CATTGGATGT TCGTACCACC AAGGAATTAC TGGAGTTAGT TGAAGCATTA 241 GGTCCCAAAA TTTGTTTACT AAAAACACAT GTGGATATCT TGACTGATTT TTCCATGGAG 301 GGCACAGTTA AGCCGCTAAA GGCATTATCC GCCAAGTACA ATTTTTTACT CTTCGAAGAC 361 AGAAAATTTG CTGACATTGG TAATACAGTC AAATTGCAGT ACTCTGCGGG TGTATACAGA 421 ATAGCAGAAT GGGCAGACAT TACGAATGCA CACGGTGTGG TGGGCCCAGG TATTGTTAGC 481 GGTTTGAAGC AGGCGGCAGA AGAAGTAACA AAGGAACCTA GAGGCCTTTT GATGTTAGCA 541 GAATTGTCAT GCAAGGGCTC CCTATCTACT GGAGAATATA CTAAGGGTAC TGTTGACATT 601 GCGAAGAGCG ACAAAGATTT TGTTATCGGC TTTATTGCTC AAAGAGACAT GGGTGGAAGA 661 GATGAAGGTT ACGATTGGTT GATTATGACA CCCGGTGTGG GTTTAGATGA CAAGGGAGAC 721 GCATTGGGTC AACAGTATAG AACCGTGGAT GATGTGGTCT CTACAGGATC TGACATTATT 781 ATTGTTGGAA GAGGACTATT TGCAAAGGGA AGGGATGCTA AGGTAGAGGG TGAACGTTAC 841 AGAAAAGCAG GCTGGGAAGC ATATTTGAGA AGATGCGGCC AGCAAAACTA AAAAAACTGT 901 ATTATAAGTA AATGCATGTA TACTAAACTC ACAAATTTAG AGCTTCAATT TAATTATATC 961 AGTTATTACC CTATGCGGGT GTGAAATACC GCACAGATGC GTAAAGGAGA AAAATACCGC 1021 ATCAGAAATT GTTAAGCGTT ATATTTTTGT TTAAAAACTC GCGATAATTT TTGTAATCAG 1081 CTCATTTTTT AACCGATTAG GCCGAAATTC GGCCAAATTG C //
Primer: URA3pro_R () Sequence file: RDB08716_A8Lcd.seq
>08716_08716_A8Lc_3_URA3pro_R_D07_10_ABI24.ab1 1 CGGTCTTTCT CACACTACAT ATGCGTATAT ATACCAATCT AAGTCTGTGC TCCTTCCTTC 61 GTTCTTCCTT CTGTTCGGAG ATTACCGAAT CAAAAAAATT TCAAGGAAAC CGAAATCAAA 121 AAAAAGAATA AAAAAAAAAT GATGAATTGA AAAGGTGGTA TGGTGCACTC TCAGTACAAT 181 CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC 241 CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG 301 CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT 361 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGGATCGCTT 421 GCCTGTAACT TACACGCGCC TCGTATCTTT TAATGATGGA ATAATTTGGG AATTTACTCT 481 GTGTTTATTT ATTTTTATGT TTTGTATTTG GATTTTAGAA AGTAAATAAA GAAGGTAGAA 541 GAGTTACGGA ATGAAGAAAA AAAAATAAAC AAAGGTTTAA AAAATTTCAA CAAAAAGCGT 601 ACTTTACATA TATATTTATT AGACAAGAAA AGCAGATTAA ATAGATATAC ATTCGATTAA 661 CGATAAGTAA AATGTAAAAT CACAGGATTT TCGTGTGTGG TCTTCTACAC AGACAAGATG 721 AAACAATTCG GCATTAATAC CTGAGAGCAG GAAGAGCAAG ATAAAAGGTA GTATTTGTTG 781 GCGATCCCCC TAGAGTCTTT TACATCTTCG GAAAACAAAA ACTATTTTTT CTTTAATTTC 841 TTTTTTTACT TTCTATTTTT AATTTATATA TTTATATTAA AAAAATTTAA ATTATAATTT 901 ATTTTTTATA GCACGTGATG AAAAGGACCC AGGTGGCACT TTTTCGGGGG AAAATGTGCG 961 CGGAAACCCC TATTTGTTTA TTTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 1021 CAATAACCCT GATAAATGCT TCATATATTG AAAAATGAGA GTATGAGTAT TCACATTTCC 1081 GTGTCGCGTA ATCCGTTTTT TGCGCATTTT GGCCTTTCCT GATTTTTTGA CT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Takeda, Y., In vitro formation of a novel type of membrane vesicles containing Dpm1p: putative transport vesicles for lipid droplets in budding yeast. J. Biochem. 143, 803-811 (2008). PMID 18343875. [link to RRC of NBRP]