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pRS316-chimeraN

Plasmid clone to express chimera N form of ERG11 in S. cerevisiae

Catalog number RDB08789
Resource name pRS316-chimeraN
Alternative name 246
Clone info. Plasmid clone to express chimera N form of ERG11 in S. cerevisiae. The chimera N contains a potential N-glycosylation site at N-terminus and 3xHA-tag at C-terminus, respectively.
Comment ERG11(283-312, with a potential N-Glycosylation site)-ERG11-3HA on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae ERG11
Depositor|Developer Nakano, Akihiko |
Other clones in our bank

External Database
S. cerevisiae ERG11

          Reference sequence
            
           
          Sequence RDB08789z.seq
          Remarks, protocol and/or map (pdf) RDB08789.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Biochem. 127, 747-754, 2000).
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          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biochem. 127, 747-754, 2000)。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB08789 pRS316-chimeraN Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pRS316-chimeraN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08789).

          Reference section:

          Homma, K., Yoshida, Y., Nakano, A., Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-Golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism. J. Biochem. 127, 747-754 (2000). PMID 10788782. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Homma, K., Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-Golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism. J. Biochem. 127, 747-754 (2000). PMID 10788782. [link to RRC of NBRP]

          2023.06.19

          GNP_filter3_RDBDEP_html_230615.pl