RDB08930 - CSIV-UbC-MCS-IRES2-Venus

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSIV-UbC-MCS-IRES2-Venus (#RDB08930)

Backbone vector plasmid of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.

Clone info.	Backbone vector plasmid of lentivirus construct driven by UbC promoter with Venus fluorescent protein marker.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSIV-UbC-MCS-IRES2-Venus (Plasmid)
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions	37C, LB+Amp
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence	RDB08930z.seq
Remarks, protocol and/or map (pdf)	RDB08930.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08930	CSIV-UbC-MCS-IRES2-Venus	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-UbC-MCS-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08930).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08930_A5C9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-F () Sequence file: RDB08930_A5C9a.seq
>08930_08930_A5C9_IRES-F_D12_12.ab1 1 CAGTCTTCTG TGTTTAGTCG AGGTTAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG 61 GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA AGGGCGAGGA 121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGCT 241 GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGGGCTA 301 CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA 421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA 541 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA 601 GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT ACCAGTCCGC 721 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC 781 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA GAATTCAAGG CCTCTCGAGC 841 CTCTAGAGTT AACATCGAGG GATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 901 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 961 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCCTCCCT 1021 TGTATAAAAT CCCTGATTGC TGTCTCTTTA TGAAGGAGTT GTGCCCCGTT GTCAGCAACG 1081 GTGGCGTGGC TGTGCACTGT GATTGCTGAA CGCAACCCCC CCCACTGGTA TTGGGGGGCC 1141 AATTGC //
Primer: IRES-R () Sequence file: RDB08930_A5C9b.seq
>08930_08930_A5C9_IRES-R_E12_15.ab1 1 GCACAGCCGG CTCGGCAGTA ACGTTAGGGG GGGGGGAGGG AGAGGGGCGG ATCCGCGGCC 61 GCTGCAGAAT TCTCGAGACC GGTAGCGCTA AACACGGTCC GGTCTAACAA AAAAGCCAAA 121 AACGGCCAGA ATTTAGCGGA CAATTTACTA GTCTAACACT GAAAATTACA TATTGACCCA 181 AATGATTACA TTTCAAAAGG TGCCTAAAAA ACTTCACAAA ACACACTCGC CAACCCCGAG 241 CGCATAGTTC AAAACCGGAG CTTCAGCTAC TTAAGAAGAT AGGTACATAA AACCGACCAA 301 AGAAACTGAC GCCTCACTTA TCCCTCCCCT CACCAGAGGT CCGGCGCCTG TCGATTCAGG 361 AGAGCCTACC CTAGGCCCGA ACCCTGCGTC CTGCGACGGA GAAAAGCCTA CCGCACACCT 421 ACCGGCAGGT GGCCCCACCC TGCATTATAA GCCAACAGAA CGGGTGACGT CACGACACGA 481 CGAGGGCGCG CGCTCCCAAA GGTACGGGTG CACTGCCCAA CGGCACCGCC ATAACTGCCG 541 CCCCCGCAAC AGACGACAAA CCGAGTTCTC CAGTCAGTGA CAAACTTCAC GTCAGGGTCC 601 CCAGATGGTG CCCCAGCCCA TCTCACCCGA ATAAGAGCTT TCCCGCATTA GCGAAGGCCT 661 CAAGACCTTG GGTTCTTGCC GCCCACCATG CCCCCCACCT TGTTTCAACG ACCTCACAGC 721 CCGCCTCACA AGCGTCTTCC ATTCAAGACT CGGGAACAGC CGCCATTTTG CTGCGCTCCC 781 CCCAACCCCC AGTTCAGGGC AACCTTGCTC GCGGACCCAG ACTACAGCCC TTGGCGGTCT 841 CTCCACACGC TTCCGTCCCA CCGAGCGGCC CGGCGGCCAC GAAAGCCCCG GCCAGCCCAG 901 CAGCCCGCTA CTCACCAAGT GACGATCACA GCGATCCACA AACAAGAACC GCGACCCAAA 961 TCCCGGCTGC GACGGAACTA GCTGTGCCAC ACCCGGCGCG TCCTTTATAT AATCATCGGC 1021 GTTCACCGCC CCACGGGAGA TCCTCGCAGA ATCGCCGAAG AAGGACTACT TTTCTTCGCT 1081 GTTCCGCCTC TCTGAAGAAA ACAAGTGACC CTAGAGGTTC ACCCAAGTTC CCGCTCCTTA 1141 AAAATGGTTT CAT //
Primer: CMV-Forward () Sequence file: RDB08930_A5C9c.seq
>08930_08930_A5C9_CMV-Forward_F12_18.ab1 1 CGTCCTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC 61 AGCGCGTTTT GCCTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC 121 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT 181 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC 241 AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG AACAGGGACT TGAAAGCGAA AGGGAAACCA 301 GAGGAGCTCT CTCGACGCAG GACTCGGCTT GCTGAAGCGC GCACGGCAAG AGGCGAGGGG 361 CGGCGACTGG TGAGTACGCC AAAAATTTTG ACTAGCGGAG GCTAGAAGGA GAGAGATGGG 421 TGCGAGAGCG TCAGTATTAA GCGGGGGAGA ATTAGATCGC GATGGGAAAA AATTCGGTTA 481 AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA 541 GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG 601 GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA 661 GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA 721 GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG CACAGCAAGC GGCCGCTGAT 781 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA 841 AGTAGTAAAA ATTGAACCAT TAGAGTAGCA CCCACCAAGG CAAAGAAGAA GAGTGTGCAG 901 AGAGAAAAAG AGCAGTGGGA TAGGAGCTTT GTTCCTTGGG TTCTTGGGGA GCAGCAGAGC 961 ACTATGGGGC GCAGCGTCAT GACGCTGACG TACAGCAGAC ATTATTGTCT GCTATATGCA 1021 GCAGCCGACC AATTTGCTGA AGGCCTAATT GAGGCCAACG CATCTGATGC ACCTCAGTCT 1081 GGGCAATCAC GCAGGCTCTC TGA //
Primer: BGH_rev () Sequence file: RDB08930_A5C9d.seq
>08930_08930_A5C9_BGHrev_G12_21.ab1 1 GATTCGGGAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA 61 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA 121 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC 181 CAGAGAGACC CAGTACAGTC CGGAATAACT TCGTATAATG TATGCTATAC GAAGTTATGA 241 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT 301 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG 361 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG 421 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG 481 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC 541 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT 601 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG 661 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC 721 AGCAGCGGGC AAGGCAGGCG GCGATGAGTT CCGCCGTGGC AATAGGGAGG GGGAAAGCGA 781 AAGTCCCGGA AAGGAGCTGA CAGGTGGTGG CAATGCCCCA ACCAGTGGGG GTTGCGTCAG 841 CAAACACAGT GCACACCACG CCACGTTGCC TGACAACGGG CCACAACTCC TCATAAAGAG 901 ACAGCAACCA GGATTTATAC AAAGAGGAGA AAATGAAAGC CATACGGGAA GCAATAGCAT 961 GATACAAAGG CATTAAAGCA GCGTATCCAC ATAGCGTAAA AGGAGCAACA TAGTTAAGAA 1021 TACCAGTTCA ATCTTTCACA AATTTTGTAA TCCAGAGGTG GATTATCGAT AAGCTTTGAT 1081 CCCCTCGATG TTACTCTAGA GCTCGAGAAG GCCTGATTCC TTACCTGTAC AGCTTCGTCC 1141 AATGCCCGAA GAAGTGAATT CCCCTAGACC GGACGGATA //
Primer: SV40pro_F () Sequence file: RDB08930_A5C9e.seq
>08930_08930_A5C9_SV40pro_F_H12_24.ab1 1 CCTGTCTGAC ATTCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT 121 CGGCATAGTA TATCGGCATA GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT 181 TGACCAGTGC CGTTCCGGTG CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA 241 CCGACCGGCT CGGGTTCTCC CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG 301 ACGACGTGAC CCTGTTCATC AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG 361 CCTGGGTGTG GGTGCGCGGC CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA 421 CGAACTTCCG GGACGCCTCC GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC 481 GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG 541 ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 601 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 661 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 721 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 781 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 841 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 901 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 961 TTTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCC AGCTGCATTA 1021 ATGATCGGCC CAACGCGCGG GGAGAGCGGT TTGCGTATTG GGGCGCTCTT CGCCTTTCTC 1081 GCCTCACTGA CTCCGCTTGC GCTTCGCTTC GCTTCCGGCC TGGCGGCGAG CGGGTAATTC 1141 CAGGCCTTCC AACCTCTCGA AAAGGC //
Primer: UbC-N_R () Sequence file: RDB08930_A5C9f.seq
>08930_08930_A9Cq_UbC-N_R_C01_07_ABI24.ab1 1 CATTAACAGC GGGGCCGCTG TCTGAGCGTC CGGGCGGAAG GATCAGGACG CTCGCTGCGC 61 CCTTCGTCTG ACGTGGCAGC GCTCGCCGTG AGGAGGGGGG CGCCCGCGGG GGGCGCCAAA 121 ACCCGGCGCG GAGGCCAGAT CTCCCGATCT CGAGCTAGAA TTCAAAGTGA ATTTCTGCTG 181 TCCCTGTAAT AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT 241 TGTATGTCTG TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC 301 CCTTTTCTTT TAAAATTGTG GATGAATACT GCCATTTGTA GATCCGTTCA CTAATCGAAT 361 GGATCTGTCT CTGTCTCTCT CTCCACCTTC TTCTTCTATT CCTTCGGGCC TGTCGGGTCC 421 CCTCGGGGTT GGGAGGTGGG TCTGAAACGA TAATGGTGAA TATCCCTGCC TAACTCTATT 481 CACTATAGAA AGTACAGCAA AAACTATTCT TAAACCTACC AAGCCTCCTA CTATCATTAT 541 GAATAATTTT ATATACCACA GCCAATTTGT TATGTTAAAC CAATTCCACA AACTTGCCCA 601 TTTATCTAAT TCCAATAATT CTTGTTCATT CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT 661 TAAGGAGTGT ATTAAGCTTG TGTAATTGTT AATTTCTCTG TCCCACTCCA TCCAGGTCGT 721 GTGATTCCAA ATCTGTTCCA GAGATTTATT ACTCCAACTA GCATTCCAAG GCACAGCAGT 781 GGTGCAAATG AGTTTTCCAG AGCAACCCCA AATCCCCAGG AGCTGTTGAT CCTTTAGGTA 841 TCTTTCCACA GCCAGGATTC TTGCCTGGAG CTGCTTGATG CCCCAGACTG TGAGTTGCAA 901 CAGATGCTGT TGCGCCTCAA TAGCCCTCAG CAAATTGTTC TGCTGCTGCA CTATACCAGA 961 CAATAAATTG TCTGGCCCTG TACCGTCAGC GTCATTTGAC GCTGCGCCCA TAGTGCTTTC 1021 CTGCTGCTCC CAAGGACCAA GGAAACAAGC TCCTATTTCC CACTGCTTCT TTTTCTCTCT 1081 GCACCACCTC GTCTCTTGCC TGATGGTTGC CTACCTCCTA TGGATTCAAG ATTTTA //