Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBlKS(-)tsA58 (#RDB09084)

Plasmid clone of temperature-sensitive mutant of simian virus 40 large T antigen.

Alternative name	stock no 5
Clone info.	Plasmid clone of the temperature-sensitive mutant (U19tsA58) of simian virus 40 large-tumor antigen.
Comment	This clone contains a genomic DNA fragment corresponding to the 2691nt to 5163nt of NC_001669.1. Known differences (at least) : C to T substitution at 3505nt (Ala to Val, ts mutation); T to C substitution at 3755nt (Leu, synonym); G to A substitution at 3930nt (Gln, synonym); C to T substitution at 4750nt (in intron); G to A substitution at 4839nt (in intron); C to T substitution at 4853nt (in intron); G to A substitution at 4879nt (in intron).
Vector backbone	Plasmid
Gene/insert name	Simian virus 40 large T antigen.
Depositor|Developer	Niwa, Hitoshi |

 

Remarks, protocol and/or map (pdf)	RDB09084p.pdf

Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB09084	pBlKS(-)tsA58	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pBlKS(-)tsA58 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09084).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB09084_B2Evp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310) Region: insert 5' Sequence file: RDB09084_B2Eva.seq
>09084_09084_B2Fv_1_pUC-M13_-100_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NGGNANGCNN GNNTTTTCCC NGTCNCGACG TTGTAAAACG    61 ACGGCCAGTG NNCNCNNNNA ATACGACTCA CTATAGGGCG AATTGGAGCT CCACCGNNGT   121 GGCGGCCGCT CTAGAACTAG TGGATCCGCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA   181 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTTTGCA AAGATGGATA AAGTTTTAAA   241 CAGAGAGGAA TCTTTGCAGC TAATGGACCT TCTAGGTCTT GAAAGGAGTG CCTGGGGGAA   301 TATTCCTCTG ATGAGAAAGG CATATTTAAA AAAATGCAAG GAGTTTCATC CTGATAAAGG   361 AGGAGATGAA GAAAAAATGA AGAAAATGAA TACTCTGTAC AAGAAAATGG AAGATGGAGT   421 AAAATATGCT CATCAACCTG ACTTTGGAGG CTTCTGGGAT GCAACTGAGG TATTTGCTTC   481 TTCCTTAAAT CCTGGTGTTG ATGCAATATA CTGCAAACAA TGGCCTGAGT GTGTAAAGAA   541 AATGTCTACT AACTGCATAT GCTTGCTGTG CTTACTGAGG ATGAAGCATG AAAATAGAAA   601 ATTATACAGG AAAGATCCAC TTGTGTGGGT TGATTGTTAC TGCTTCGATT GCTTTANAAT   661 GTGGTTTGGA CTTGATCTTT GTGAAGGAAC CTTACTTCTG TGGTGTGACA TAATTGGACA   721 AACTACCTAC AGAGATTTAA AGCTCTAANG TAAATATAAA ATTTTTAAGT GTATAATGTG   781 TTAAACTACT GATTCTAATT GTTTGTGTAT TTTANATTCC NACCTATGGA ACTGATGAAT   841 GGNNNCAGTG GTGGAATGCC TTTAATGAGG AAAACNNGTT TTGCTCANAA GAAATGNNNT   901 CTANNGATGA TGANGNTACN GCTNNNTNNN NNCATTCTAN NCCNNNNAAA ANNANGANNA 961 AGGNAGANNA NNNNNANNNN NNTTNNNNCN NNNNNNNNN //
Primer: pUC-CAPbs_up_F2 (Pr0739) Region: T3 pro,insert 3' Sequence file: RDB09084_B2Evb.seq
>09084_09084_B2Fv_1_pUC-CAPbs_up_F2_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GCTTNNNNCT TNATGCTTCC GGCTCGTATG TTGTGTGGAA    61 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG   121 CAATTAACCC TCACTAAAGG GAACAAAAGC TGGGTACCGG GCCCCCCCTC GAGGTCGACG   181 GTATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG ATCCGAACAA CAACAATTGC   241 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC   301 CTCTACAAAT GTGGTATGGC TGATTATGAT CATGAACAGA CTGTGAGGAC TGAGGGGCCT   361 GAAATGAGCC TTGGGACTGT GAATCAATGC CTGTTTCATG CCCTGAGTCT TCCATGTTCT   421 TCTCCCCACC ATCTTCATTT TTATCAGCAT TTTCCTGGCT GTCTTCATCA TCATCATCAC   481 TGTTTCTTAG CCAATCTAAA ACTCCAATTC CCATAGCCAC ATTAAACTTC ATTTTTTGAT   541 ACACTGACAA ACTAAACTCT TTGTCCAATC TCTCTTTCCA CTCCACAATT CTGCTCTGAA   601 TACTTTGAGC AAACTCAGCC ACAGGTCTGT ACCAAATTAA CATAAGAAGC AAAGCAATGC   661 CACTTTGAAT TATTCTCTTT TCTAACAAAA ACTCACTGCG TTCCAGGCAA TGCTTTAAAT   721 AATCTTTGGG CCTAAAATCT ATTTGTTTTA CAAATCTGGC CTGCAGTGTT TTAGGCACAC   781 TGTACTCATT CATGGTGACT ATTCCAGGGG GAAATATTTG AGTTCTTTTA TTTANGTGTT   841 TCTTTTCTAA NTTTACCTTA NCACTGCCAT CCNAATAATC CCNNAAATTG TCCAGGTTAN   901 NNATTCCCNG ANCTGNANNN CAAATCTCTG GNNTCCCNTC CAGNGCCCTT TNNNTNCNTC 961 NAAAACTACT NAAAAANTNG NNCNNNANNN NNNNNNN //
Primer: LTA_M_F (Pr0270) Region: insert mid Sequence file: RDB09084_B2Evc.seq
>09084_09084_B2Fv_1_LTA_M_F_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNTNNN NNNTCTGGCC TGCAGTGTTT TAGGCACACT    61 GTACTCATTC ATGGTGACTA TTCCAGGGGG AAATATTTGA GTTCTTTTAT TTAGGTGTTT   121 CTTTTCTAAG TTTACCTTAA CACTGCCATC CAAATAATCC CTTAAATTGT CCAGGTTATT   181 AATTCCCTGA CCTGAAGGCA AATCTCTGGA CTCCCCTCCA GTGCCCTTTA CATCCTCAAA   241 AACTACTAAA AACTGGTCAA TAGCTACTCC TAGCTCAAAG TTCAGCCTGT CCAAGGGCAA   301 ATTAACATTT AAAGCTTTCC CCCCACATAA TTCAAGCAAA ACAGCTGCTA ATGTAGTTTT   361 ACCACTATCA ATTGGTCCTT TAAACAGCCA GTATCTTTTT TTAGGAATGT TGTACACCAT   421 GCATTTTAAA AAGTCATACA CCACTGAATC CATTTTGGGC AACAAACAGT GTAGCCAAGC   481 AACTCCAGCC ATCCATTCTT CTATGTCAGC AGAGCCTGTA GAACCAAACA TTATATCCAT   541 CCTATCCAAA AGATCATTAA ATCTGTTTGT TAACATTTGT TCTCTAGTTA GTTGTAGGCT   601 ATCAACCCGC TTTTTAGCTA AAACAGTATC AACAGCCTGT TGGCATATGG TTTTTTGGTT   661 TTTGCTGTCA GCAAATATAG CAGCATTTGC ATAATGCTTT TCATGGTACT TATAGTGGCT   721 GGGCTGTTCT TTTTTAATAC ATTTTAAACA CATTTCAAAA CTGTATTGAA ATTCCAAGTA   781 CATCCCAAGC AATAACAACA CATCATCACA TTTTGTTTCC ATTGCATACT CTGTTACAAG   841 CTTCCAGGAN ACTTGTTTAG TTTCCTCTGC TTCTTCTGGA NTAAAATCNT GCTCCNTTAA   901 CCCACCNGGC AAACTTTCCT CNNNAACANA AAATGGATCT CTANNNNNNG CANNATACAT   961 CAANATTCCT NATTNNNCCN NTTNNAANTN AAAAAGCTAA ANGNNNNNNN TTTTTGANCN 1021 NANTNNNTNA NNANCANNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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Other resources in our bank

• simian virus 40 large T antigen and ortholog

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External Database
simian virus 40 large T antigen

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