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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmPGKpacECFPpA

Expression vector of pac-ECFP with mPGK promoter.

Catalog number RDB10118
Resource name pmPGKpacECFPpA
Alternative name stock no 1095
Clone info. Expression vector of pac-ECFP with mPGK promoter and poly(A).
Vector backbone pBIKS(-) (Plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Depositor|Developer Niwa, Hitoshi |
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External Database

            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB10118p.pdf

            Distribution information

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            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
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            RDB10118 pmPGKpacECFPpA DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pmPGKpacECFPpA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB10118).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB10118_B3E9p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: f1ori_F2 (Pr0308)
            Region: T7 pro,insert 5'(PGK pro)
            Sequence file: RDB10118_B3E9a.seq check
            >10118_10118_B3E9_3_f1ori_F2_E01_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNTN NNGNNTTCGN NCTATTGGTT AAAAAATGAG CTGANTTAAC
               61 AAAAATTTNN NNNNNNNNTT ANCAAAATAT TAACGCTTAC AATTTCCATT CNNNATTCAG
              121 GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC
              181 GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG
              241 ACGTTGTAAA ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA
              301 GCTCCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC AGGAATTCTA
              361 CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT
              421 GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAG
              481 GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT
              541 CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC
              601 ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT
              661 TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA GGCTGGGAAG
              721 GGGTGGGTCC GGGGGCGGGC TCANGGGCGG GCTCANGGGC GGGGCGGGCG CCCGAANGTC
              781 CTCCGGANGC CCGGCATTCT GCACGCTTCA AAANCGCACG TCTGCCGCGC TGTTCTCCTC
              841 TTNCTCATCT CCNGGCNTTT CNANCGANCA TCNAGCTNGA TCNTCANGAN CGANNACNAG
              901 CCCNCNGNGN NNCNNNNNAC CCNNNANNAN NTCCCCAGGG NCNNNACNNN CCNNCNNNGN
            961 NNNNNTNNNN CNNANNNNCC NNNNNNNNNN
            //
            Primer: SV40pA3element Reverse (Pr0108)
            Region: insert 3'(PGK pA)
            Sequence file: RDB10118_B3E9b.seq check
            >10118_10118_B3E9_3_SV40pA3'element Reverse_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNGNGNNNNN ATNNNNNNNC NNGCAACGCG GCCTTTNNNN NNTTCCTGGC
               61 CTTTTGCNNN NNCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
              121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA
              181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC
              241 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG
              301 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA
              361 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA
              421 GCTATGACCA TGATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG
              481 GTACCGGGCC CCCCCTCGAG GTCGACGGAT CCAGATCTAT AGATCATGAG TGGGAGGAAT
              541 GAGCTGGCCC TTAATTTGGT TTTGCTTGTT TAAATTATGA TATCCAACTA TGAAACATTA
              601 TCATAAAGCA ATAGTAAAGA GCCTTCAGTA AAGAGCAGGC ATTTATCTAA TCCCACCCCA
              661 CCCCCACCCC CGTAGCTCCA ATCCTTCCAT TCAAAATGTA NGTACTCTGT TCTCACCCTT
              721 CTTAACAAAG TATGACAGGA AAAACTTCCA TGTGGACATC TTTATTGTTT AATAGATCAT
              781 CAATTTCTCG ACTATTCCTT TGCCCTCNGA CGANTGCTGG GGCGTCNNNT TCCACTATCG
              841 GCGANTCGAT AANCTGGCCG CTTTANTTGN ACAGCNCGNC CATGCCGANN GNGNTNCNNG
              901 NNGNNNGNNN NNANNNNNNN CNNNANCNNN NNNTCNCNNN TNNNCNNNGG GGNCTTNNNN
            961 NNNGNNNGNN NNNNNNNN
            //
            Primer: PGKpro_F (Pr0249)
            Region: insert mid(PuroR 3',ECFP 5')
            Sequence file: RDB10118_B3E9c.seq check
            >10118_10118_B3E9_3_PGKpro_F_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNTCTCCN NGCCTTTCGA CCGNTCATCN AGCTTGATCC
               61 TCATGACCGA GTNNCNANNC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC
              121 GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG
              181 GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC
              241 GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG
              301 GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC
              361 GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG
              421 GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG
              481 GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC
              541 CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC
              601 GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC
              661 GGATCGACGG TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG
              721 GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGANCTGG ACGGCGACGT AAACGGCCAC
              781 AAGTTCAGCG TGTCCGGCGA GGGCGANGGC GANGCCNCCT ACNGCAAGCT GANCCTGNAN
              841 TTCNTCTGCA CCACCGGCAA GCTGCCCGNG CCCNNNNCCA CCNNCNNGNN CNCCNNNNNN
              901 NGGGGNGNGC ANNGNTTCNG CCNCTACCCN NANNNCNTGN ANCNNCNCNA NNTNNNTNNN
            961 NNNNNNN
            //
            Primer: T3 (Pr0112)
            Region: insert mid(PGK pA,ECFP 3')
            Sequence file: RDB10118_B3E9d.seq check
            >10118_10118_B3E9_3_T3_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNCCNNN NNNGNCGNNN GNNCNNGATC TATAGATCAT GAGTGGGAGG
               61 AATGAGCTGG NCCNNNNNTT GGTTTTGCTT GTTTAAATTA TGATATCCAA CTATGAAACA
              121 TTATCATAAA GCAATAGTAA AGAGCCTTCA GTAAAGAGCA GGCATTTATC TAATCCCACC
              181 CCACCCCCAC CCCCGTAGCT CCAATCCTTC CATTCAAAAT GTAGGTACTC TGTTCTCACC
              241 CTTCTTAACA AAGTATGACA GGAAAAACTT CCATGTGGAC ATCTTTATTG TTTAATAGAT
              301 CATCAATTTC TCGACTATTC CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA
              361 TCGGCGAGTC GATAAGCTGG CCGCTTTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC
              421 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC
              481 TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA
              541 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC
              601 GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACGTTGT
              661 GGCTGATGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA
              721 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGNG
              781 TCTTGTAGTT GCCGTCGNCC TTGAAGAANA NGGTGCGCTC CTGGNCGTAN CCTTCNGGCN
              841 TGGCGGANTN GAAGAANTCG NGCTGCTTCN TGTGGNCNGG GNANCGGCNN ANNCACTGCA
            901 CGCCCCANNN CNNNNNNNNA CNNNGNNGGG CNANGGNNNG GNNNCTNGNN NNNNNNNN
            //
            Primer: SK (Pr0103)
            Region: insert mid(PGK pro,PuroR 5')
            Sequence file: RDB10118_B3E9e.seq check
            >10118_10118_B3E9_3_SK_F01_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG GGNANGGGNN GNGCTTTTCC NNAGGNAGTC TGGAGCATGC
               61 GCTTTAGCAG CCCCNNTGGG NACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT
              121 TCCACATCCA CCGGTAGGCG CCAACCGGCT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT
              181 TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCAGGACG
              241 TGACAAATGG AAGTAGCACG TCTCACTAGT CTCGTGCAGA TGGACAGCAC CGCTGAGCAA
              301 TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC CAATAGCAGC TTTGCTCCTT CGCTTTCTGG
              361 GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG
              421 GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG GCATTCTGCA CGCTTCAAAA GCGCACGTCT
              481 GCCGCGCTGT TCTCCTCTTC CTCATCTCCG GGCCTTTCGA CCGATCATCA AGCTTGATCC
              541 TCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG
              601 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG
              661 ANCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG
              721 ACATCGGCAA GGNGTGGGTC GCGNANGANG GCGCCGCGGN GGCGGTCTGG NNCACGCCGG
              781 ANAGCGTCNA ANCGGGGGCG GNGTTCGCCG ANNTCGGNCC GCGCATGNNC NANTTGANCN
              841 GNNNNCCGGN TGNNCNNNNA NCNNCNGATG GNANNNCNNN CNNNNNNNNN ANNNGNCCCN
            901 ANGNANCNNN NNNNNNN
            //
            Primer: PuroR_F (Pr0554)
            Region: insert mid(ECFP 5')
            Sequence file: RDB10118_B3E9f.seq check
            >10118_10118_B3E9_3_PuroR_F_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNCNNNNNN NCNGNNNGNN CGCGCACCTG GTGCATGACC CGCAAGCCCG
               61 GTGNCNNNNN CNNNNGTACC GCGGGCCCGG GNTCCACCGG TCGCCACCAT GGTGAGCAAG
              121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
              181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
              241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
              301 CTGACCTGGG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
              361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
              421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
              481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
              541 AACTACATCA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC
              601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
              661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGANCACC
              721 CAGTCCGCCC TGANCAAAGA NCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGANTTC
              781 GTGACCGCCG CCNGGNTCAC TCTCGGCATG GNNGANCTGT ACNAGTAAAG CGGCCAGCTT
              841 ATCGACTCGC CNATANNGGA AACCGNNGCC CCANCACTCN NNCNANGGCA AAGGNNNANN
              901 CNANAANNTG ATGANCTANT NNANNNNNAA NANGNCCNNN NNGNNANNTT TTNNNNNNNN
            961 NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2024.03.26

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